CXCR4 down-regulation by small interfering RNA inhibits invasion and tubule formation of human retinal microvascular endothelial cells

Keming Yu, Jing Zhuang, Joseph M. Kaminski, Bala Ambati, Qianying Gao, Ping Ma, Dongjiang Liao, Fan Li, Xuan Liu, Jian Ge

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The progressive alterations to the retinal microvasculature in diabetic retinopathy are known to cause vision loss. Chemokines are characterized by their ability to induce cell invasion, adhesion and migration. In this study, we used double siRNA transfection to transiently and selectively decrease the level of the endogenous CXCR4 in human retinal microvascular endothelial cells (HRMECs). The functional consequences of silencing CXCR4 expression in HRMECs were investigated using an endothelial cell migration assay and tubule formation in Matrigel. When CXCR4 expression was decreased with siRNA, HRMECs were less invasive and also resulted in markedly diminished vascular networks on Matrigel as compared to the controls. Additionally, hypoxia and VEGF, the factors affecting microvascular, regulate the expression level of CXCR4 in HRMECs, respectively, which have synergistic, additive effect in the HRMECs. As such, CXCR4 antagonists may become a therapeutic target for the treatment of retinal angiopathies.

Original languageEnglish (US)
Pages (from-to)990-996
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume358
Issue number4
DOIs
StatePublished - Jul 13 2007

Fingerprint

Endothelial cells
Small Interfering RNA
Down-Regulation
Endothelial Cells
Cell Migration Assays
Aptitude
Cell adhesion
Diabetic Retinopathy
Microvessels
Chemokines
Cell Adhesion
Vascular Endothelial Growth Factor A
Cell Movement
Transfection
Blood Vessels
Assays
Therapeutics

Keywords

  • CXCR4
  • Human retinal microvascular endothelial cells
  • Hypoxia
  • VEGF
  • siRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

CXCR4 down-regulation by small interfering RNA inhibits invasion and tubule formation of human retinal microvascular endothelial cells. / Yu, Keming; Zhuang, Jing; Kaminski, Joseph M.; Ambati, Bala; Gao, Qianying; Ma, Ping; Liao, Dongjiang; Li, Fan; Liu, Xuan; Ge, Jian.

In: Biochemical and Biophysical Research Communications, Vol. 358, No. 4, 13.07.2007, p. 990-996.

Research output: Contribution to journalArticle

Yu, Keming ; Zhuang, Jing ; Kaminski, Joseph M. ; Ambati, Bala ; Gao, Qianying ; Ma, Ping ; Liao, Dongjiang ; Li, Fan ; Liu, Xuan ; Ge, Jian. / CXCR4 down-regulation by small interfering RNA inhibits invasion and tubule formation of human retinal microvascular endothelial cells. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 358, No. 4. pp. 990-996.
@article{2692f5ef34bc471db69a44c1decfa3f8,
title = "CXCR4 down-regulation by small interfering RNA inhibits invasion and tubule formation of human retinal microvascular endothelial cells",
abstract = "The progressive alterations to the retinal microvasculature in diabetic retinopathy are known to cause vision loss. Chemokines are characterized by their ability to induce cell invasion, adhesion and migration. In this study, we used double siRNA transfection to transiently and selectively decrease the level of the endogenous CXCR4 in human retinal microvascular endothelial cells (HRMECs). The functional consequences of silencing CXCR4 expression in HRMECs were investigated using an endothelial cell migration assay and tubule formation in Matrigel. When CXCR4 expression was decreased with siRNA, HRMECs were less invasive and also resulted in markedly diminished vascular networks on Matrigel as compared to the controls. Additionally, hypoxia and VEGF, the factors affecting microvascular, regulate the expression level of CXCR4 in HRMECs, respectively, which have synergistic, additive effect in the HRMECs. As such, CXCR4 antagonists may become a therapeutic target for the treatment of retinal angiopathies.",
keywords = "CXCR4, Human retinal microvascular endothelial cells, Hypoxia, VEGF, siRNA",
author = "Keming Yu and Jing Zhuang and Kaminski, {Joseph M.} and Bala Ambati and Qianying Gao and Ping Ma and Dongjiang Liao and Fan Li and Xuan Liu and Jian Ge",
year = "2007",
month = "7",
day = "13",
doi = "10.1016/j.bbrc.2007.05.004",
language = "English (US)",
volume = "358",
pages = "990--996",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - CXCR4 down-regulation by small interfering RNA inhibits invasion and tubule formation of human retinal microvascular endothelial cells

AU - Yu, Keming

AU - Zhuang, Jing

AU - Kaminski, Joseph M.

AU - Ambati, Bala

AU - Gao, Qianying

AU - Ma, Ping

AU - Liao, Dongjiang

AU - Li, Fan

AU - Liu, Xuan

AU - Ge, Jian

PY - 2007/7/13

Y1 - 2007/7/13

N2 - The progressive alterations to the retinal microvasculature in diabetic retinopathy are known to cause vision loss. Chemokines are characterized by their ability to induce cell invasion, adhesion and migration. In this study, we used double siRNA transfection to transiently and selectively decrease the level of the endogenous CXCR4 in human retinal microvascular endothelial cells (HRMECs). The functional consequences of silencing CXCR4 expression in HRMECs were investigated using an endothelial cell migration assay and tubule formation in Matrigel. When CXCR4 expression was decreased with siRNA, HRMECs were less invasive and also resulted in markedly diminished vascular networks on Matrigel as compared to the controls. Additionally, hypoxia and VEGF, the factors affecting microvascular, regulate the expression level of CXCR4 in HRMECs, respectively, which have synergistic, additive effect in the HRMECs. As such, CXCR4 antagonists may become a therapeutic target for the treatment of retinal angiopathies.

AB - The progressive alterations to the retinal microvasculature in diabetic retinopathy are known to cause vision loss. Chemokines are characterized by their ability to induce cell invasion, adhesion and migration. In this study, we used double siRNA transfection to transiently and selectively decrease the level of the endogenous CXCR4 in human retinal microvascular endothelial cells (HRMECs). The functional consequences of silencing CXCR4 expression in HRMECs were investigated using an endothelial cell migration assay and tubule formation in Matrigel. When CXCR4 expression was decreased with siRNA, HRMECs were less invasive and also resulted in markedly diminished vascular networks on Matrigel as compared to the controls. Additionally, hypoxia and VEGF, the factors affecting microvascular, regulate the expression level of CXCR4 in HRMECs, respectively, which have synergistic, additive effect in the HRMECs. As such, CXCR4 antagonists may become a therapeutic target for the treatment of retinal angiopathies.

KW - CXCR4

KW - Human retinal microvascular endothelial cells

KW - Hypoxia

KW - VEGF

KW - siRNA

UR - http://www.scopus.com/inward/record.url?scp=34249719900&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34249719900&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.05.004

DO - 10.1016/j.bbrc.2007.05.004

M3 - Article

C2 - 17521613

AN - SCOPUS:34249719900

VL - 358

SP - 990

EP - 996

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -