Cyclosporine effect on ornithine decarboxylase activity in rat submaxillary lymph nodes. Modulation by parasympathetic nerves

Ana I. Esquifino, Ruth E. Rosenstein, Javier Eduardo Stern, Daniel P. Cardinali

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

This study was performed: (1) to assess whether cyclosporine affected the increase in submaxillary lymph node ornithine decarboxylase activity induced by complete Freund's adjuvant injection to rats bearing a regional parasympathetic decentralization; (2) to examine the effect of cyclosporine on cholinergic markers in submaxillary lymph nodes of rats injected with complete Freund's adjuvant or its vehicle. The unilateral parasympathetic decentralization of the submaxillary lymph nodes was achieved by unilateral chorda tympani section; each rat was also contralaterally sham-operated. A 73% decrease of choline acetyltransferase activity and a 78% decrease of neuronal [3H]choline uptake, were found in the ipsilateral side 2 weeks after surgery. Cyclosporine (5 or 20 mg/kg) or its vehicle was s.c. injected once daily for 5 days. Freund's complete adjuvant or its vehicle was injected s.c. 1 h before the 3rd injection of cyclosporine. The animals were killed 2 h after the last cyclosporine injection. Parasympathetic decentralization of the submaxillary lymph nodes was followed, 2 weeks later, by a significant inhibition of cyclosporine activity on Freund's adjuvant-induced ornithine decarboxylase. In rats injected with complete Freund's adjuvant or its vehicle, sympathetic denervation of submaxillary lymph nodes achieved by unilateral superior cervical ganglionectomy, augmented the stimulatory activity of cyclosporine on choline acetyltransferase and neuronal choline uptake. Cyclosporine treatment did not modify the total [3H]choline uptake, regardless of Freund's adjuvant injection or of intactness of the sympathetic innervation. Choline acetyltransferase and choline uptake were significantly augmented by the injection of Freund's adjuvant alone. The results indicate that an appropriate parasympathetic local environment is needed for cyclosporine immunomodulation in lymphoid tissue, and that cyclosporine augmented cholinergic activity in submaxillary lymph nodes, an effect amplified by regional sympathetic denervation. Cholinergic markers in lymphoid tissue increased during the immune reaction.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalEuropean Journal of Pharmacology
Volume254
Issue number1-2
DOIs
StatePublished - Mar 11 1994

Fingerprint

Ornithine Decarboxylase
Cyclosporine
Freund's Adjuvant
Lymph Nodes
Choline
Choline O-Acetyltransferase
Politics
Injections
Cholinergic Agents
Sympathectomy
Lymphoid Tissue
Ganglionectomy
Immunomodulation

Keywords

  • Acetylcholine, synthesis and release
  • Choline uptake
  • Cholinergic innervation
  • Cyclosporine
  • Lymphoid tissue
  • Neuroimmunomodulation
  • Parasympathetic nervous system
  • Superior cervical ganglion
  • Sympathetic nervous system

ASJC Scopus subject areas

  • Pharmacology

Cite this

Cyclosporine effect on ornithine decarboxylase activity in rat submaxillary lymph nodes. Modulation by parasympathetic nerves. / Esquifino, Ana I.; Rosenstein, Ruth E.; Stern, Javier Eduardo; Cardinali, Daniel P.

In: European Journal of Pharmacology, Vol. 254, No. 1-2, 11.03.1994, p. 1-7.

Research output: Contribution to journalArticle

Esquifino, Ana I. ; Rosenstein, Ruth E. ; Stern, Javier Eduardo ; Cardinali, Daniel P. / Cyclosporine effect on ornithine decarboxylase activity in rat submaxillary lymph nodes. Modulation by parasympathetic nerves. In: European Journal of Pharmacology. 1994 ; Vol. 254, No. 1-2. pp. 1-7.
@article{b0ae05f9174946bf8794e5de0db8f687,
title = "Cyclosporine effect on ornithine decarboxylase activity in rat submaxillary lymph nodes. Modulation by parasympathetic nerves",
abstract = "This study was performed: (1) to assess whether cyclosporine affected the increase in submaxillary lymph node ornithine decarboxylase activity induced by complete Freund's adjuvant injection to rats bearing a regional parasympathetic decentralization; (2) to examine the effect of cyclosporine on cholinergic markers in submaxillary lymph nodes of rats injected with complete Freund's adjuvant or its vehicle. The unilateral parasympathetic decentralization of the submaxillary lymph nodes was achieved by unilateral chorda tympani section; each rat was also contralaterally sham-operated. A 73{\%} decrease of choline acetyltransferase activity and a 78{\%} decrease of neuronal [3H]choline uptake, were found in the ipsilateral side 2 weeks after surgery. Cyclosporine (5 or 20 mg/kg) or its vehicle was s.c. injected once daily for 5 days. Freund's complete adjuvant or its vehicle was injected s.c. 1 h before the 3rd injection of cyclosporine. The animals were killed 2 h after the last cyclosporine injection. Parasympathetic decentralization of the submaxillary lymph nodes was followed, 2 weeks later, by a significant inhibition of cyclosporine activity on Freund's adjuvant-induced ornithine decarboxylase. In rats injected with complete Freund's adjuvant or its vehicle, sympathetic denervation of submaxillary lymph nodes achieved by unilateral superior cervical ganglionectomy, augmented the stimulatory activity of cyclosporine on choline acetyltransferase and neuronal choline uptake. Cyclosporine treatment did not modify the total [3H]choline uptake, regardless of Freund's adjuvant injection or of intactness of the sympathetic innervation. Choline acetyltransferase and choline uptake were significantly augmented by the injection of Freund's adjuvant alone. The results indicate that an appropriate parasympathetic local environment is needed for cyclosporine immunomodulation in lymphoid tissue, and that cyclosporine augmented cholinergic activity in submaxillary lymph nodes, an effect amplified by regional sympathetic denervation. Cholinergic markers in lymphoid tissue increased during the immune reaction.",
keywords = "Acetylcholine, synthesis and release, Choline uptake, Cholinergic innervation, Cyclosporine, Lymphoid tissue, Neuroimmunomodulation, Parasympathetic nervous system, Superior cervical ganglion, Sympathetic nervous system",
author = "Esquifino, {Ana I.} and Rosenstein, {Ruth E.} and Stern, {Javier Eduardo} and Cardinali, {Daniel P.}",
year = "1994",
month = "3",
day = "11",
doi = "10.1016/0014-2999(94)90362-X",
language = "English (US)",
volume = "254",
pages = "1--7",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Cyclosporine effect on ornithine decarboxylase activity in rat submaxillary lymph nodes. Modulation by parasympathetic nerves

AU - Esquifino, Ana I.

AU - Rosenstein, Ruth E.

AU - Stern, Javier Eduardo

AU - Cardinali, Daniel P.

PY - 1994/3/11

Y1 - 1994/3/11

N2 - This study was performed: (1) to assess whether cyclosporine affected the increase in submaxillary lymph node ornithine decarboxylase activity induced by complete Freund's adjuvant injection to rats bearing a regional parasympathetic decentralization; (2) to examine the effect of cyclosporine on cholinergic markers in submaxillary lymph nodes of rats injected with complete Freund's adjuvant or its vehicle. The unilateral parasympathetic decentralization of the submaxillary lymph nodes was achieved by unilateral chorda tympani section; each rat was also contralaterally sham-operated. A 73% decrease of choline acetyltransferase activity and a 78% decrease of neuronal [3H]choline uptake, were found in the ipsilateral side 2 weeks after surgery. Cyclosporine (5 or 20 mg/kg) or its vehicle was s.c. injected once daily for 5 days. Freund's complete adjuvant or its vehicle was injected s.c. 1 h before the 3rd injection of cyclosporine. The animals were killed 2 h after the last cyclosporine injection. Parasympathetic decentralization of the submaxillary lymph nodes was followed, 2 weeks later, by a significant inhibition of cyclosporine activity on Freund's adjuvant-induced ornithine decarboxylase. In rats injected with complete Freund's adjuvant or its vehicle, sympathetic denervation of submaxillary lymph nodes achieved by unilateral superior cervical ganglionectomy, augmented the stimulatory activity of cyclosporine on choline acetyltransferase and neuronal choline uptake. Cyclosporine treatment did not modify the total [3H]choline uptake, regardless of Freund's adjuvant injection or of intactness of the sympathetic innervation. Choline acetyltransferase and choline uptake were significantly augmented by the injection of Freund's adjuvant alone. The results indicate that an appropriate parasympathetic local environment is needed for cyclosporine immunomodulation in lymphoid tissue, and that cyclosporine augmented cholinergic activity in submaxillary lymph nodes, an effect amplified by regional sympathetic denervation. Cholinergic markers in lymphoid tissue increased during the immune reaction.

AB - This study was performed: (1) to assess whether cyclosporine affected the increase in submaxillary lymph node ornithine decarboxylase activity induced by complete Freund's adjuvant injection to rats bearing a regional parasympathetic decentralization; (2) to examine the effect of cyclosporine on cholinergic markers in submaxillary lymph nodes of rats injected with complete Freund's adjuvant or its vehicle. The unilateral parasympathetic decentralization of the submaxillary lymph nodes was achieved by unilateral chorda tympani section; each rat was also contralaterally sham-operated. A 73% decrease of choline acetyltransferase activity and a 78% decrease of neuronal [3H]choline uptake, were found in the ipsilateral side 2 weeks after surgery. Cyclosporine (5 or 20 mg/kg) or its vehicle was s.c. injected once daily for 5 days. Freund's complete adjuvant or its vehicle was injected s.c. 1 h before the 3rd injection of cyclosporine. The animals were killed 2 h after the last cyclosporine injection. Parasympathetic decentralization of the submaxillary lymph nodes was followed, 2 weeks later, by a significant inhibition of cyclosporine activity on Freund's adjuvant-induced ornithine decarboxylase. In rats injected with complete Freund's adjuvant or its vehicle, sympathetic denervation of submaxillary lymph nodes achieved by unilateral superior cervical ganglionectomy, augmented the stimulatory activity of cyclosporine on choline acetyltransferase and neuronal choline uptake. Cyclosporine treatment did not modify the total [3H]choline uptake, regardless of Freund's adjuvant injection or of intactness of the sympathetic innervation. Choline acetyltransferase and choline uptake were significantly augmented by the injection of Freund's adjuvant alone. The results indicate that an appropriate parasympathetic local environment is needed for cyclosporine immunomodulation in lymphoid tissue, and that cyclosporine augmented cholinergic activity in submaxillary lymph nodes, an effect amplified by regional sympathetic denervation. Cholinergic markers in lymphoid tissue increased during the immune reaction.

KW - Acetylcholine, synthesis and release

KW - Choline uptake

KW - Cholinergic innervation

KW - Cyclosporine

KW - Lymphoid tissue

KW - Neuroimmunomodulation

KW - Parasympathetic nervous system

KW - Superior cervical ganglion

KW - Sympathetic nervous system

UR - http://www.scopus.com/inward/record.url?scp=0028230624&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028230624&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(94)90362-X

DO - 10.1016/0014-2999(94)90362-X

M3 - Article

C2 - 8206106

AN - SCOPUS:0028230624

VL - 254

SP - 1

EP - 7

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1-2

ER -