Cytochemical studies on peroxisomes in rat and guinea pig myocardium

Goro Asano, Muhammad Ashraf

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The enzymatic activity and distribution of peroxisomes (microbodies) in rat and guinea pig hearts were studied cytochemically, by means of oxidation of 3-3′-diaminobenzidine (DAB) and by using B-glycerophosphate and cytidine-5′-monophosphate as substrates. Peroxisomes were localized in proximity to mitochondria and sarcoplasmic reticulum and measured from 0.2 μrn to 0.5 μm in diameter in both animal species. DAB positive bodies were seen both at pH 9.0 and pH 5.0 in rat myocardial cells. However, in guinea pig myocardial cells the reaction was observed only at pH 9.0, or very faintly at pH 5.0. Acid and alkaline phosphatases were not demonstrated in the peroxisomes. Lipid droplets were surrounded by a ring of dense granular reaction product for enzymes, such as acid and alkaline phosphatase, and lipofuscin granules were limited by acid phosphatase or DAB reaction products. The pathophysiological function of peroxisomes is discussed.

Original languageEnglish (US)
Pages (from-to)205-212
Number of pages8
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume34
Issue number1
DOIs
StatePublished - Dec 1980
Externally publishedYes

Keywords

  • Acid phosphatase
  • Alkaline phosphatase
  • Catalase
  • Lipid metabolism
  • Microbodies
  • Myocardium
  • Peroxisome
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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