Cytotoxicity of Denture Base Resins

Paul J. Sheridan, Sreenivas Koka, Nels O. Ewoldsen, Carol A. Lefebvre, Marshall T. Lavin

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

This in vitro study examined the effect of eluate from heat-activated, chemically activated, and microwave-activated denture base resins on cell viability of primary cultures of human gingival fibroblasts. Eluates corresponding to 24, 48, 72, and 96 hours of resin disk immersion were prepared. Fibroblasts were plated at a density of 3 × 104 cells in 96-well plates and exposed to a medium containing eluate. After 24 hours, the cytotoxic: effect was determined by cellular mitochondrial function. The effect of eluates was compared to control cultures containing culture medium without eluate. Results indicated that at all time periods tested, all three resins leached materials that were cytotoxic to the fibroblasts. Eluate from chemically activated resin disks was more cytotoxic than eluate from heat-activated and microwave-activated disks. In general, cytotoxicity appeared to diminish as disk immersion time was increased. The greatest cytotoxic. effect on cell viability was observed with eluates recovered after 24 hours of disk immersion, and the least cytotoxic effect was observed with eluates recovered after 96 hours of immersion.

Original languageEnglish (US)
Pages (from-to)73-77
Number of pages5
JournalInternational Journal of Prosthodontics
Volume10
Issue number1
StatePublished - Dec 1 1997

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Denture Bases
Immersion
Fibroblasts
Microwaves
Cell Survival
Hot Temperature
Culture Media

ASJC Scopus subject areas

  • Oral Surgery

Cite this

Sheridan, P. J., Koka, S., Ewoldsen, N. O., Lefebvre, C. A., & Lavin, M. T. (1997). Cytotoxicity of Denture Base Resins. International Journal of Prosthodontics, 10(1), 73-77.

Cytotoxicity of Denture Base Resins. / Sheridan, Paul J.; Koka, Sreenivas; Ewoldsen, Nels O.; Lefebvre, Carol A.; Lavin, Marshall T.

In: International Journal of Prosthodontics, Vol. 10, No. 1, 01.12.1997, p. 73-77.

Research output: Contribution to journalArticle

Sheridan, PJ, Koka, S, Ewoldsen, NO, Lefebvre, CA & Lavin, MT 1997, 'Cytotoxicity of Denture Base Resins', International Journal of Prosthodontics, vol. 10, no. 1, pp. 73-77.
Sheridan PJ, Koka S, Ewoldsen NO, Lefebvre CA, Lavin MT. Cytotoxicity of Denture Base Resins. International Journal of Prosthodontics. 1997 Dec 1;10(1):73-77.
Sheridan, Paul J. ; Koka, Sreenivas ; Ewoldsen, Nels O. ; Lefebvre, Carol A. ; Lavin, Marshall T. / Cytotoxicity of Denture Base Resins. In: International Journal of Prosthodontics. 1997 ; Vol. 10, No. 1. pp. 73-77.
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