DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation

Yi Xie, Yan Hong, Xiao Yue Ma, Xiu Rong Ren, Susan Ackerman, Lin Mei, Wen Cheng Xiong

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Netrins, a family of secreted molecules, play important roles in axon pathfinding during nervous system development. Although phosphatidylinositol signaling has been implicated in this event, how netrin-1 regulates phosphatidylinositol signaling remains poorly understood. Here we provide evidence that netrin-1 stimulates phosphatidylinositol bisphosphate hydrolysis in cortical neurons. This event appears to be mediated by DCC (deleted in colorectal cancer), but not neogenin or Unc5h2. Netrin-1 induces phospholipase Cγ (PLCγ) tyrosine phosphorylation. Inhibition of PLC activity attenuates netrin-1-induced cortical neurite outgrowth. These results suggest that netrin-1 regulates phosphatidylinositol turnover and demonstrate a crucial role of PLC signaling in netrin-1-induced neurite elongation.

Original languageEnglish (US)
Pages (from-to)2605-2611
Number of pages7
JournalJournal of Biological Chemistry
Volume281
Issue number5
DOIs
StatePublished - Feb 3 2006

Fingerprint

Type C Phospholipases
Neurites
Elongation
Colorectal Neoplasms
Phosphatidylinositols
Phosphorylation
Neurology
Nervous System
Neurons
Tyrosine
netrin-1
Hydrolysis
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Xie, Y., Hong, Y., Ma, X. Y., Ren, X. R., Ackerman, S., Mei, L., & Xiong, W. C. (2006). DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation. Journal of Biological Chemistry, 281(5), 2605-2611. https://doi.org/10.1074/jbc.M512767200

DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation. / Xie, Yi; Hong, Yan; Ma, Xiao Yue; Ren, Xiu Rong; Ackerman, Susan; Mei, Lin; Xiong, Wen Cheng.

In: Journal of Biological Chemistry, Vol. 281, No. 5, 03.02.2006, p. 2605-2611.

Research output: Contribution to journalArticle

Xie, Y, Hong, Y, Ma, XY, Ren, XR, Ackerman, S, Mei, L & Xiong, WC 2006, 'DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation', Journal of Biological Chemistry, vol. 281, no. 5, pp. 2605-2611. https://doi.org/10.1074/jbc.M512767200
Xie, Yi ; Hong, Yan ; Ma, Xiao Yue ; Ren, Xiu Rong ; Ackerman, Susan ; Mei, Lin ; Xiong, Wen Cheng. / DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 5. pp. 2605-2611.
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