DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation

Yi Xie; Yan Hong; Xiao Yue Ma; Xiu Rong Ren; Susan Ackerman; Lin Mei; Wen Cheng Xiong

doi:10.1074/jbc.M512767200

DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation

Yi Xie, Yan Hong, Xiao Yue Ma, Xiu Rong Ren, Susan Ackerman, Lin Mei, Wen Cheng Xiong

Research output: Contribution to journal › Article › peer-review

50 Scopus citations

Abstract

Netrins, a family of secreted molecules, play important roles in axon pathfinding during nervous system development. Although phosphatidylinositol signaling has been implicated in this event, how netrin-1 regulates phosphatidylinositol signaling remains poorly understood. Here we provide evidence that netrin-1 stimulates phosphatidylinositol bisphosphate hydrolysis in cortical neurons. This event appears to be mediated by DCC (deleted in colorectal cancer), but not neogenin or Unc5h2. Netrin-1 induces phospholipase Cγ (PLCγ) tyrosine phosphorylation. Inhibition of PLC activity attenuates netrin-1-induced cortical neurite outgrowth. These results suggest that netrin-1 regulates phosphatidylinositol turnover and demonstrate a crucial role of PLC signaling in netrin-1-induced neurite elongation.

Original language	English (US)
Pages (from-to)	2605-2611
Number of pages	7
Journal	Journal of Biological Chemistry
Volume	281
Issue number	5
DOIs	https://doi.org/10.1074/jbc.M512767200
State	Published - Feb 3 2006
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1074/jbc.M512767200

Cite this

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title = "DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation",

abstract = "Netrins, a family of secreted molecules, play important roles in axon pathfinding during nervous system development. Although phosphatidylinositol signaling has been implicated in this event, how netrin-1 regulates phosphatidylinositol signaling remains poorly understood. Here we provide evidence that netrin-1 stimulates phosphatidylinositol bisphosphate hydrolysis in cortical neurons. This event appears to be mediated by DCC (deleted in colorectal cancer), but not neogenin or Unc5h2. Netrin-1 induces phospholipase Cγ (PLCγ) tyrosine phosphorylation. Inhibition of PLC activity attenuates netrin-1-induced cortical neurite outgrowth. These results suggest that netrin-1 regulates phosphatidylinositol turnover and demonstrate a crucial role of PLC signaling in netrin-1-induced neurite elongation.",

author = "Yi Xie and Yan Hong and Ma, {Xiao Yue} and Ren, {Xiu Rong} and Susan Ackerman and Lin Mei and Xiong, {Wen Cheng}",

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AU - Xie, Yi

AU - Hong, Yan

AU - Ma, Xiao Yue

AU - Ren, Xiu Rong

AU - Ackerman, Susan

AU - Mei, Lin

AU - Xiong, Wen Cheng

PY - 2006/2/3

Y1 - 2006/2/3

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AB - Netrins, a family of secreted molecules, play important roles in axon pathfinding during nervous system development. Although phosphatidylinositol signaling has been implicated in this event, how netrin-1 regulates phosphatidylinositol signaling remains poorly understood. Here we provide evidence that netrin-1 stimulates phosphatidylinositol bisphosphate hydrolysis in cortical neurons. This event appears to be mediated by DCC (deleted in colorectal cancer), but not neogenin or Unc5h2. Netrin-1 induces phospholipase Cγ (PLCγ) tyrosine phosphorylation. Inhibition of PLC activity attenuates netrin-1-induced cortical neurite outgrowth. These results suggest that netrin-1 regulates phosphatidylinositol turnover and demonstrate a crucial role of PLC signaling in netrin-1-induced neurite elongation.

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DCC-dependent phospholipase C signaling in netrin-1-induced neurite elongation

Abstract

ASJC Scopus subject areas

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