Decreased RhoA expression in myocardium of diabetic rats

Jiping Tang, Sharyn M. Fitzgerald, Brandi N. Boughtman, Samuel W. Cole, Michael W Brands, John H. Zhang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Diabetic cardiomyopathy is one of the major causes of death in diabetic patients, but the pathogenesis is unclear. There is evidence that RhoA, a small GTPase, might be involved in cardiac function. This study, therefore, analyzed RhoA expression and activation in hearts of diabetic rats. Male Sprague-Dawley rats were divided into control and diabetic groups of 18 each. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). Rats were studied 3 weeks after induction of diabetes. Heart rate, which was measured 24 h/day, decreased by 93 ± 7 beats/min in diabetic rats. There was a 62% decrease (p < 0.01) in RhoA mRNA expression in heart tissues (left ventricle) of diabetic rats (38.5 ± 6.7 × 106 molecules/μg total RNA) compared with controls (101 ± 10.3 × 106 molecules/μg total RNA). Western blot showed a 33% decrease in total RhoA protein expression in heart tissues of diabetic rats compared with controls (p < 0.05). A reduced RhoA translocation in heart tissues of diabetic rats was determined by a 64% decrease in membrane-bound RhoA (p < 0.01 vs. control group), indicating that the activation of RhoA is markedly reduced in diabetic myocardium. Our data suggest that down-regulated RhoA may be involved in cardiomyopathy in diabetic rats.

Original languageEnglish (US)
Pages (from-to)775-783
Number of pages9
JournalCanadian Journal of Physiology and Pharmacology
Volume83
Issue number8-9
DOIs
StatePublished - Aug 1 2005

Fingerprint

Myocardium
Diabetic Cardiomyopathies
rhoA GTP-Binding Protein
RNA
Control Groups
Monomeric GTP-Binding Proteins
Streptozocin
Intravenous Injections
Heart Ventricles
Sprague Dawley Rats
Cause of Death
Heart Rate
Western Blotting
Messenger RNA
Membranes

Keywords

  • Diabetes
  • Heart
  • RhoA

ASJC Scopus subject areas

  • Physiology
  • Pharmacology
  • Physiology (medical)

Cite this

Tang, J., Fitzgerald, S. M., Boughtman, B. N., Cole, S. W., Brands, M. W., & Zhang, J. H. (2005). Decreased RhoA expression in myocardium of diabetic rats. Canadian Journal of Physiology and Pharmacology, 83(8-9), 775-783. https://doi.org/10.1139/y05-077

Decreased RhoA expression in myocardium of diabetic rats. / Tang, Jiping; Fitzgerald, Sharyn M.; Boughtman, Brandi N.; Cole, Samuel W.; Brands, Michael W; Zhang, John H.

In: Canadian Journal of Physiology and Pharmacology, Vol. 83, No. 8-9, 01.08.2005, p. 775-783.

Research output: Contribution to journalArticle

Tang, J, Fitzgerald, SM, Boughtman, BN, Cole, SW, Brands, MW & Zhang, JH 2005, 'Decreased RhoA expression in myocardium of diabetic rats', Canadian Journal of Physiology and Pharmacology, vol. 83, no. 8-9, pp. 775-783. https://doi.org/10.1139/y05-077
Tang, Jiping ; Fitzgerald, Sharyn M. ; Boughtman, Brandi N. ; Cole, Samuel W. ; Brands, Michael W ; Zhang, John H. / Decreased RhoA expression in myocardium of diabetic rats. In: Canadian Journal of Physiology and Pharmacology. 2005 ; Vol. 83, No. 8-9. pp. 775-783.
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