Deficiency of renal cortical EGF increases ENaC activity and contributes to salt-sensitive hypertension

Tengis S. Pavlov, V. Levchenko, Paul M O'Connor, Daria V. Ilatovskaya, Oleg Palygin, Takefumi Mori, David L. Mattson, Andrey Sorokin, Julian H. Lombard, Allen W. Cowley, Alexander Staruschenko

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Various stimuli, including hormones and growth factors, modulate epithelial sodium channels (ENaCs), which fine-tune Na+ absorption in the kidney. Members of the EGF family are important for maintaining transepithelial Na+ transport, but whether EGF influences ENaC, perhaps mediating salt-sensitive hypertension, is not well understood. Here, the ENaC inhibitor benzamil attenuated the development of hypertension in Dahl salt-sensitive rats. Feeding these salt-sensitive rats a high-salt diet led to lower levels of EGF in the kidney cortex and enhanced the expression and activity of ENaC compared with feeding a lowsalt diet. To directly evaluate the role of EGF in the development of hypertension and its effect on ENaC activity, we infused EGF intravenously while continuously monitoring BP of the salt-sensitive rats. Infusion of EGF decreased ENaC activity, prevented the development of hypertension, and attenuated glomerular and renal tubular damage. Taken together, these findings indicate that cortical EGF levels decrease with a high-salt diet in salt-sensitive rats, promoting ENaC-mediated Na+ reabsorption in the collecting duct and the development of hypertension.

Original languageEnglish (US)
Pages (from-to)1053-1062
Number of pages10
JournalJournal of the American Society of Nephrology
Volume24
Issue number7
DOIs
StatePublished - Jun 28 2013

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Epidermal Growth Factor
Salts
Hypertension
Kidney
Diet
Inbred Dahl Rats
Epithelial Sodium Channels
Kidney Cortex
Intercellular Signaling Peptides and Proteins
Hormones

ASJC Scopus subject areas

  • Nephrology

Cite this

Deficiency of renal cortical EGF increases ENaC activity and contributes to salt-sensitive hypertension. / Pavlov, Tengis S.; Levchenko, V.; O'Connor, Paul M; Ilatovskaya, Daria V.; Palygin, Oleg; Mori, Takefumi; Mattson, David L.; Sorokin, Andrey; Lombard, Julian H.; Cowley, Allen W.; Staruschenko, Alexander.

In: Journal of the American Society of Nephrology, Vol. 24, No. 7, 28.06.2013, p. 1053-1062.

Research output: Contribution to journalArticle

Pavlov, TS, Levchenko, V, O'Connor, PM, Ilatovskaya, DV, Palygin, O, Mori, T, Mattson, DL, Sorokin, A, Lombard, JH, Cowley, AW & Staruschenko, A 2013, 'Deficiency of renal cortical EGF increases ENaC activity and contributes to salt-sensitive hypertension', Journal of the American Society of Nephrology, vol. 24, no. 7, pp. 1053-1062. https://doi.org/10.1681/ASN.2012080839
Pavlov, Tengis S. ; Levchenko, V. ; O'Connor, Paul M ; Ilatovskaya, Daria V. ; Palygin, Oleg ; Mori, Takefumi ; Mattson, David L. ; Sorokin, Andrey ; Lombard, Julian H. ; Cowley, Allen W. ; Staruschenko, Alexander. / Deficiency of renal cortical EGF increases ENaC activity and contributes to salt-sensitive hypertension. In: Journal of the American Society of Nephrology. 2013 ; Vol. 24, No. 7. pp. 1053-1062.
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