Delayed repair of transected nerves: Effect of brain-derived neurotrophic factor

Melinda S. Moir, Michelle Z. Wang, Michael To, Joanne Lum, David J Terris

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: To determine if administration of brain-derived neurotrophic factor (BDNF) after peripheral nerve transection can improve the functional outcome in situations where epineurial repair must be delayed. Design: Randomized, blinded, controlled trial. Subjects: Thirty-four Sprague-Dawley rats. Intervention: Sciatic nerves were transected and, after a 2-week delay, repaired with epineurial sutures. Animals were assigned to receive daily administration of lactated Ringer solution (LR [control] group); BDNF delivered at the time of nerve transection through 2 weeks after nerve repair, for a total of 4 weeks (BDNF-early group); or BDNF delivered at the time of nerve repair through 2 weeks after repair (BDNF-late group). Outcome was assessed using sciatic functional indices (SFIs) and histomorphometric analysis. Results: The SFI maximal recovery was superior in the BDNF groups, but this difference did not reach statistical significance (SFI, -90.1 ± 9.6 [LR group], -85.7 ± 7.6 [BDNF-early group], and-84.6 ± 4.8 [BDNF-late group], where normal function is 0 and complete loss of function is -100; P = .27). The mean axon diameter tended to be greater in the BDNF groups compared with the LR group, ie, 2.43 ± 0.23 μm (LR group), 2.80 ± 0.44 μm (BDNF- early group), and 2.83 ± 0.38 μm (BDNF-late group) (P = .05). Conclusions: The local administration of BDNF to nerves that underwent transection and then repair after a delay resulted in an increase in axonal diameters and maximal SFIs, a difference that did not reach statistical significance. The timing of BDNF administration after nerve transection did not affect neuronal regeneration.

Original languageEnglish (US)
Pages (from-to)501-505
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume126
Issue number4
DOIs
StatePublished - Jan 1 2000

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Brain-Derived Neurotrophic Factor
Sciatic Nerve
Peripheral Nerves
Sutures
Sprague Dawley Rats
Axons
Regeneration
Randomized Controlled Trials

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

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Delayed repair of transected nerves : Effect of brain-derived neurotrophic factor. / Moir, Melinda S.; Wang, Michelle Z.; To, Michael; Lum, Joanne; Terris, David J.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 126, No. 4, 01.01.2000, p. 501-505.

Research output: Contribution to journalArticle

Moir, Melinda S. ; Wang, Michelle Z. ; To, Michael ; Lum, Joanne ; Terris, David J. / Delayed repair of transected nerves : Effect of brain-derived neurotrophic factor. In: Archives of Otolaryngology - Head and Neck Surgery. 2000 ; Vol. 126, No. 4. pp. 501-505.
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abstract = "Objective: To determine if administration of brain-derived neurotrophic factor (BDNF) after peripheral nerve transection can improve the functional outcome in situations where epineurial repair must be delayed. Design: Randomized, blinded, controlled trial. Subjects: Thirty-four Sprague-Dawley rats. Intervention: Sciatic nerves were transected and, after a 2-week delay, repaired with epineurial sutures. Animals were assigned to receive daily administration of lactated Ringer solution (LR [control] group); BDNF delivered at the time of nerve transection through 2 weeks after nerve repair, for a total of 4 weeks (BDNF-early group); or BDNF delivered at the time of nerve repair through 2 weeks after repair (BDNF-late group). Outcome was assessed using sciatic functional indices (SFIs) and histomorphometric analysis. Results: The SFI maximal recovery was superior in the BDNF groups, but this difference did not reach statistical significance (SFI, -90.1 ± 9.6 [LR group], -85.7 ± 7.6 [BDNF-early group], and-84.6 ± 4.8 [BDNF-late group], where normal function is 0 and complete loss of function is -100; P = .27). The mean axon diameter tended to be greater in the BDNF groups compared with the LR group, ie, 2.43 ± 0.23 μm (LR group), 2.80 ± 0.44 μm (BDNF- early group), and 2.83 ± 0.38 μm (BDNF-late group) (P = .05). Conclusions: The local administration of BDNF to nerves that underwent transection and then repair after a delay resulted in an increase in axonal diameters and maximal SFIs, a difference that did not reach statistical significance. The timing of BDNF administration after nerve transection did not affect neuronal regeneration.",
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N2 - Objective: To determine if administration of brain-derived neurotrophic factor (BDNF) after peripheral nerve transection can improve the functional outcome in situations where epineurial repair must be delayed. Design: Randomized, blinded, controlled trial. Subjects: Thirty-four Sprague-Dawley rats. Intervention: Sciatic nerves were transected and, after a 2-week delay, repaired with epineurial sutures. Animals were assigned to receive daily administration of lactated Ringer solution (LR [control] group); BDNF delivered at the time of nerve transection through 2 weeks after nerve repair, for a total of 4 weeks (BDNF-early group); or BDNF delivered at the time of nerve repair through 2 weeks after repair (BDNF-late group). Outcome was assessed using sciatic functional indices (SFIs) and histomorphometric analysis. Results: The SFI maximal recovery was superior in the BDNF groups, but this difference did not reach statistical significance (SFI, -90.1 ± 9.6 [LR group], -85.7 ± 7.6 [BDNF-early group], and-84.6 ± 4.8 [BDNF-late group], where normal function is 0 and complete loss of function is -100; P = .27). The mean axon diameter tended to be greater in the BDNF groups compared with the LR group, ie, 2.43 ± 0.23 μm (LR group), 2.80 ± 0.44 μm (BDNF- early group), and 2.83 ± 0.38 μm (BDNF-late group) (P = .05). Conclusions: The local administration of BDNF to nerves that underwent transection and then repair after a delay resulted in an increase in axonal diameters and maximal SFIs, a difference that did not reach statistical significance. The timing of BDNF administration after nerve transection did not affect neuronal regeneration.

AB - Objective: To determine if administration of brain-derived neurotrophic factor (BDNF) after peripheral nerve transection can improve the functional outcome in situations where epineurial repair must be delayed. Design: Randomized, blinded, controlled trial. Subjects: Thirty-four Sprague-Dawley rats. Intervention: Sciatic nerves were transected and, after a 2-week delay, repaired with epineurial sutures. Animals were assigned to receive daily administration of lactated Ringer solution (LR [control] group); BDNF delivered at the time of nerve transection through 2 weeks after nerve repair, for a total of 4 weeks (BDNF-early group); or BDNF delivered at the time of nerve repair through 2 weeks after repair (BDNF-late group). Outcome was assessed using sciatic functional indices (SFIs) and histomorphometric analysis. Results: The SFI maximal recovery was superior in the BDNF groups, but this difference did not reach statistical significance (SFI, -90.1 ± 9.6 [LR group], -85.7 ± 7.6 [BDNF-early group], and-84.6 ± 4.8 [BDNF-late group], where normal function is 0 and complete loss of function is -100; P = .27). The mean axon diameter tended to be greater in the BDNF groups compared with the LR group, ie, 2.43 ± 0.23 μm (LR group), 2.80 ± 0.44 μm (BDNF- early group), and 2.83 ± 0.38 μm (BDNF-late group) (P = .05). Conclusions: The local administration of BDNF to nerves that underwent transection and then repair after a delay resulted in an increase in axonal diameters and maximal SFIs, a difference that did not reach statistical significance. The timing of BDNF administration after nerve transection did not affect neuronal regeneration.

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