Bone loss in aging is linked with chronic low-grade inflammation and the accumulation of marrowfat in animals and humans. Peroxisome proliferator-Activated receptor gamma (PPARγ), an adipogenic regulator, plays key roles in these biological processes. However, studies of the roles of PPARγin age-related bone loss and inflammation are lacking. We hypothesized that deletion of PPARγin bone marrow mesenchymal lineage cells would reduce bone loss with aging, potentially through a reduction in fat-generated inflammatory responses and an increase in osteoblastic activity. In the present study, we show that mice deficient of PPARγin Dermo1-expressing mesenchymal lineage cells (Dermo1-Cre:PPARγ?fl/fl) have reduced fat mass and increased cortical bone thickness but that deficiency of PPARγhad limited effect on protection of trabecular bone with aging as demonstrated by dual-energy X-ray absorptiometry, μCT, and histomorphometric analyses. Conditional knockout of PPARγreduced serum concentrations of adipokines, including adiponectin, resistin, and leptin, and reduced marrow stromal cell expression levels of inflammation-related genes. Inflammation genes involved in the interferon signaling pathway were reduced the most. These results demonstrate that disruption of the master adipogenic regulator, PPARγ, has a certain protective effect on aging-induced bone loss, suggesting that regulation of adipose function and modulation of interferon signaling are among the key mechanisms by which PPARγregulates bone homeostasis during aging process.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journals of Gerontology - Series A Biological Sciences and Medical Sciences|
|State||Published - Apr 17 2020|
- Bone loss
ASJC Scopus subject areas
- Geriatrics and Gerontology