Depletion-replenishment of the testicular estrogen receptor

Sensitivity to cycloheximide and actinomycin D

Tom O. Abney, Brooks Allen Keel, Russell B. Myers

Research output: Contribution to journalArticle

Abstract

Male rats (30-35 days old) were utilized to examine the process of depletion and replenishment of the testicular cytosolic estrogen receptor and to investigate the effects of cycloheximide and actinomycin D on these processes. The dose dependence and temporal nature of receptor depletion and replenishment were investigated. Maximum depletion (> 90%) occurred by 1 h after in vivo administration of either 5 or 10 μg estradiol-17β. Depletion of the cytosolic receptor at 1 h occurred concomitant to a marked increase in nuclear receptor thus indicating translocation. Receptor replenishment to control levels was observed by 6 h post treatment. To determine the requirements for transcriptional and translational events in the replenishment process, actinomycin D and cycloheximide were administered in vivo. Simultaneous treatment with cycloheximide and estradiol resulted in a significant inhibition of replenishment at 6 and 12 h post treatment of 46 and 60% below control levels, respectively. Cycloheximide treatment alone had no effect on receptor levels. A significant inhibition of replenishment at 6 h was also shown when cycloheximide was given 3 h after estradiol treatment. Cycloheximide administration at 6 h after estradiol significantly suppressed receptor levels at 12 h suggesting that replenished receptor levels at 6-12 h are in a state of rapid turnover. Receptor replenishment exhibited a different response to actinomycin D treatment in that significant inhibition was observed only when the drug was administered at 3 h after estradiol treatment. These results demonstrate that testicular cytosolic estrogen receptor depletion is dose and time dependent. The results further demonstrate that receptor replenishment involves protein synthesis and suggests that synthesis of new RNA might also be required.

Original languageEnglish (US)
Pages (from-to)989-995
Number of pages7
JournalJournal of Steroid Biochemistry
Volume24
Issue number5
DOIs
StatePublished - Jan 1 1986

Fingerprint

Dactinomycin
Cycloheximide
Estrogen Receptors
Estradiol
Level control
Cytoplasmic and Nuclear Receptors
Rats
RNA
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

Depletion-replenishment of the testicular estrogen receptor : Sensitivity to cycloheximide and actinomycin D. / Abney, Tom O.; Keel, Brooks Allen; Myers, Russell B.

In: Journal of Steroid Biochemistry, Vol. 24, No. 5, 01.01.1986, p. 989-995.

Research output: Contribution to journalArticle

@article{410cb2ffebc84261b17d6e69944d2b81,
title = "Depletion-replenishment of the testicular estrogen receptor: Sensitivity to cycloheximide and actinomycin D",
abstract = "Male rats (30-35 days old) were utilized to examine the process of depletion and replenishment of the testicular cytosolic estrogen receptor and to investigate the effects of cycloheximide and actinomycin D on these processes. The dose dependence and temporal nature of receptor depletion and replenishment were investigated. Maximum depletion (> 90{\%}) occurred by 1 h after in vivo administration of either 5 or 10 μg estradiol-17β. Depletion of the cytosolic receptor at 1 h occurred concomitant to a marked increase in nuclear receptor thus indicating translocation. Receptor replenishment to control levels was observed by 6 h post treatment. To determine the requirements for transcriptional and translational events in the replenishment process, actinomycin D and cycloheximide were administered in vivo. Simultaneous treatment with cycloheximide and estradiol resulted in a significant inhibition of replenishment at 6 and 12 h post treatment of 46 and 60{\%} below control levels, respectively. Cycloheximide treatment alone had no effect on receptor levels. A significant inhibition of replenishment at 6 h was also shown when cycloheximide was given 3 h after estradiol treatment. Cycloheximide administration at 6 h after estradiol significantly suppressed receptor levels at 12 h suggesting that replenished receptor levels at 6-12 h are in a state of rapid turnover. Receptor replenishment exhibited a different response to actinomycin D treatment in that significant inhibition was observed only when the drug was administered at 3 h after estradiol treatment. These results demonstrate that testicular cytosolic estrogen receptor depletion is dose and time dependent. The results further demonstrate that receptor replenishment involves protein synthesis and suggests that synthesis of new RNA might also be required.",
author = "Abney, {Tom O.} and Keel, {Brooks Allen} and Myers, {Russell B.}",
year = "1986",
month = "1",
day = "1",
doi = "10.1016/0022-4731(86)90351-1",
language = "English (US)",
volume = "24",
pages = "989--995",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
issn = "0960-0760",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Depletion-replenishment of the testicular estrogen receptor

T2 - Sensitivity to cycloheximide and actinomycin D

AU - Abney, Tom O.

AU - Keel, Brooks Allen

AU - Myers, Russell B.

PY - 1986/1/1

Y1 - 1986/1/1

N2 - Male rats (30-35 days old) were utilized to examine the process of depletion and replenishment of the testicular cytosolic estrogen receptor and to investigate the effects of cycloheximide and actinomycin D on these processes. The dose dependence and temporal nature of receptor depletion and replenishment were investigated. Maximum depletion (> 90%) occurred by 1 h after in vivo administration of either 5 or 10 μg estradiol-17β. Depletion of the cytosolic receptor at 1 h occurred concomitant to a marked increase in nuclear receptor thus indicating translocation. Receptor replenishment to control levels was observed by 6 h post treatment. To determine the requirements for transcriptional and translational events in the replenishment process, actinomycin D and cycloheximide were administered in vivo. Simultaneous treatment with cycloheximide and estradiol resulted in a significant inhibition of replenishment at 6 and 12 h post treatment of 46 and 60% below control levels, respectively. Cycloheximide treatment alone had no effect on receptor levels. A significant inhibition of replenishment at 6 h was also shown when cycloheximide was given 3 h after estradiol treatment. Cycloheximide administration at 6 h after estradiol significantly suppressed receptor levels at 12 h suggesting that replenished receptor levels at 6-12 h are in a state of rapid turnover. Receptor replenishment exhibited a different response to actinomycin D treatment in that significant inhibition was observed only when the drug was administered at 3 h after estradiol treatment. These results demonstrate that testicular cytosolic estrogen receptor depletion is dose and time dependent. The results further demonstrate that receptor replenishment involves protein synthesis and suggests that synthesis of new RNA might also be required.

AB - Male rats (30-35 days old) were utilized to examine the process of depletion and replenishment of the testicular cytosolic estrogen receptor and to investigate the effects of cycloheximide and actinomycin D on these processes. The dose dependence and temporal nature of receptor depletion and replenishment were investigated. Maximum depletion (> 90%) occurred by 1 h after in vivo administration of either 5 or 10 μg estradiol-17β. Depletion of the cytosolic receptor at 1 h occurred concomitant to a marked increase in nuclear receptor thus indicating translocation. Receptor replenishment to control levels was observed by 6 h post treatment. To determine the requirements for transcriptional and translational events in the replenishment process, actinomycin D and cycloheximide were administered in vivo. Simultaneous treatment with cycloheximide and estradiol resulted in a significant inhibition of replenishment at 6 and 12 h post treatment of 46 and 60% below control levels, respectively. Cycloheximide treatment alone had no effect on receptor levels. A significant inhibition of replenishment at 6 h was also shown when cycloheximide was given 3 h after estradiol treatment. Cycloheximide administration at 6 h after estradiol significantly suppressed receptor levels at 12 h suggesting that replenished receptor levels at 6-12 h are in a state of rapid turnover. Receptor replenishment exhibited a different response to actinomycin D treatment in that significant inhibition was observed only when the drug was administered at 3 h after estradiol treatment. These results demonstrate that testicular cytosolic estrogen receptor depletion is dose and time dependent. The results further demonstrate that receptor replenishment involves protein synthesis and suggests that synthesis of new RNA might also be required.

UR - http://www.scopus.com/inward/record.url?scp=0022886572&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022886572&partnerID=8YFLogxK

U2 - 10.1016/0022-4731(86)90351-1

DO - 10.1016/0022-4731(86)90351-1

M3 - Article

VL - 24

SP - 989

EP - 995

JO - Journal of Steroid Biochemistry and Molecular Biology

JF - Journal of Steroid Biochemistry and Molecular Biology

SN - 0960-0760

IS - 5

ER -