Detection of hepatitis B surface protein by means of laser spectroscopy

Alex Dergachev, Anatoliy Kudryavtsev, Sergey Mirov, Michael McCracken

Research output: Contribution to journalArticle

Abstract

The purpose of this experiment is to detect small concentrations of purified (HBsAg) hepatitis B surface antigen in a buffered salt solution. Such possibilities could be provided by means of optical spectroscopy, which includes laser-induced self-luminescence and Raman spectroscopy. Luminescence spectroscopy under selective laser excitation is a very sensitive method to determine the presence and concentration of proteins in solutions. In the experiment, UV laser light at 266 nm, which corresponds to the absorption band of Tryptophan and Tyrosine groups, is used. As a detection system TE-cooled CCD camera attached to 0.15-m spectrograph is used. Such combination provided extremely high sensitivity and relatively good spectral resolution (≥4 cm-1).

Original languageEnglish (US)
Pages (from-to)158-159
Number of pages2
JournalConference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS
StatePublished - Jan 1 1996
EventProceedings of the 1996 Conference on Lasers and Electro-Optics, CLEO'96 - Anaheim, CA, USA
Duration: Jun 2 1996Jun 7 1996

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Laser spectroscopy
Hepatitis B Surface Antigens
Luminescence
Membrane Proteins
Proteins
Laser excitation
Spectrographs
Lasers
Spectral resolution
Antigens
CCD cameras
Tryptophan
Tyrosine
Raman spectroscopy
Absorption spectra
Salts
Experiments
Spectroscopy
Optical spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Electrical and Electronic Engineering

Cite this

Detection of hepatitis B surface protein by means of laser spectroscopy. / Dergachev, Alex; Kudryavtsev, Anatoliy; Mirov, Sergey; McCracken, Michael.

In: Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS, 01.01.1996, p. 158-159.

Research output: Contribution to journalArticle

Dergachev, Alex ; Kudryavtsev, Anatoliy ; Mirov, Sergey ; McCracken, Michael. / Detection of hepatitis B surface protein by means of laser spectroscopy. In: Conference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS. 1996 ; pp. 158-159.
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