Determination of hemoglobin oxygen saturation from turbid media using reflectance spectroscopy with small source-detector separations

Maureen Johns, Cole A. Giller, Hanli Liu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In this study, we present an empirically modified diffusion model determining hemoglobin oxygen saturation from turbid media using steady-state, broadband (500-600 nm) reflectance with source-detector separations of a few hundred micrometers. Development of this model was conducted using Monte Carlo simulations, a gold standard modeling technique for predicting the behavior of light propagation through turbid media. Hemoglobin oxygen saturation levels of 0 and 100% at different blood concentrations were studied. Nonlinear curve fitting was used to extract the hemoglobin oxygen saturation values from the reflectance spectra, producing errors of 5% between the simulated curves and the model for both saturation cases. Further validation was performed using liquid-tissue phantoms containing intralipid and blood. Curve fitting between the in vitro data and the model produced errors of less than 2%. This validated model was then used to extract saturation values from in vivo reflectance spectra of the human index finger and human brain tissues. This empirically modified diffusion model provides the possibility of extracting local hemoglobin oxygen saturation from blood-perfused turbid media using reflectance data measured with a small source-detector separation probe.

Original languageEnglish (US)
Pages (from-to)1686-1694
Number of pages9
JournalApplied Spectroscopy
Volume55
Issue number12
DOIs
StatePublished - Dec 1 2001
Externally publishedYes

Fingerprint

Hemoglobin oxygen saturation
hemoglobin
Spectroscopy
Detectors
saturation
reflectance
detectors
oxygen
spectroscopy
blood
Blood
curve fitting
Curve fitting
Tissue
Light propagation
brain
Light sources
micrometers
Brain
broadband

Keywords

  • Absorption
  • Empirically modified diffusion model
  • Hemoglobin oxygen saturation
  • Monte Carlo simulations
  • Reflectance spectroscopy

ASJC Scopus subject areas

  • Instrumentation
  • Spectroscopy

Cite this

Determination of hemoglobin oxygen saturation from turbid media using reflectance spectroscopy with small source-detector separations. / Johns, Maureen; Giller, Cole A.; Liu, Hanli.

In: Applied Spectroscopy, Vol. 55, No. 12, 01.12.2001, p. 1686-1694.

Research output: Contribution to journalArticle

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