Determination of human PDGF-B promoter binding nuclear protein by capillary electrophoresis

Xingjun Fan, J. Liu, Y. X. Jin, D. B. Wang

Research output: Contribution to journalArticle

Abstract

With the use of 1.0% T, 0% C linear polyacrylamide as sieving matrix, 0.25 x TBE(Tris 89 mmol/L, boric acid 89 mmol/L, EDTA 2 mmol/L) as running buffer and 15 degrees C as column temperature, the human PDGF-B promoter binding nuclear protein can be determined within 50 min with good resolution. The results proved that there are two proteins having strong ability binding human PDGF-B promoter, which similar to that in slab gel electrophoresis. This technique can provide one of the rapid and accurate separation methods in the studying of the formation and repression behavior of DNA binding protein based on PDGF gene as the target.

Original languageEnglish (US)
Pages (from-to)170-172
Number of pages3
JournalSe pu = Chinese journal of chromatography / Zhongguo hua xue hui
Volume19
Issue number2
StatePublished - Jan 1 2001
Externally publishedYes

Fingerprint

Capillary electrophoresis
Boric acid
Ethylenediaminetetraacetic acid
DNA-Binding Proteins
Capillary Electrophoresis
Nuclear Proteins
Polyacrylates
Electrophoresis
Binding energy
Edetic Acid
Carrier Proteins
Buffers
DNA
Gels
Genes
Proteins
Aptitude
Temperature
polyacrylamide
boric acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Chemical Engineering(all)
  • Organic Chemistry
  • Electrochemistry

Cite this

Determination of human PDGF-B promoter binding nuclear protein by capillary electrophoresis. / Fan, Xingjun; Liu, J.; Jin, Y. X.; Wang, D. B.

In: Se pu = Chinese journal of chromatography / Zhongguo hua xue hui, Vol. 19, No. 2, 01.01.2001, p. 170-172.

Research output: Contribution to journalArticle

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