Development of in vivo ventilatory and single chemosensitive neuron responses to hypercapnia in rats

C. E. Stunden, J. A. Filosa, A. J. Garcia, J. B. Dean, R. W. Putnam

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

We used pressure plethysmography to study breathing patterns of neonatal and adult rats acutely exposed to elevated levels of CO2. Ventilation (V̇E) increased progressively with increasing inspired CO2. The rise in V̇E was associated with an increase in tidal volume, but not respiratory rate. In all animals studied, the CO2 sensitivity (determined from the slope of the V̇E vs. inspired % CO2 curve) was variable on a day to day basis. Chemosensitivity was high in neonates 1 day after birth (P1) and fell throughout the first week to a minimum at about P8. Chemosensitivity rose again to somewhat higher values in P10 through adult rats. The developmental pattern of these in vivo ventilatory responses was different than individual locus coeruleus (LC) neuron responses to increased CO2. The membrane potential (Vm) of LC neurons was measured using perforated patch (amphotericin B) techniques in brain slices. At all ages studied, LC neurons increased their firing rate by ∼44% in response to hypercapnic acidosis (10% CO2, pH 7.0). Thus the in vivo ventilatory response to hypercapnia was not correlated with the Vm response of individual LC neurons to hypercapnic acidosis in neonatal rats. These data suggest that CO2 sensitivity of ventilation in rats may exist in two forms, a high-sensitivity neonatal (or fetal) form and a lower-sensitivity adult form, with a critical window of very low sensitivity during the period of transition between the two (∼P8).

Original languageEnglish (US)
Pages (from-to)135-155
Number of pages21
JournalRespiration Physiology
Volume127
Issue number2-3
DOIs
StatePublished - Aug 18 2001
Externally publishedYes

Fingerprint

Locus Coeruleus
Hypercapnia
Ventilation
Neurons
Acidosis
Plethysmography
Tidal Volume
Amphotericin B
Respiratory Rate
Membrane Potentials
Respiration
Parturition
Pressure
Brain

Keywords

  • CO
  • CO Response
  • Carbon dioxide
  • Central chemosensitivity
  • Chemosensitivity
  • Control of breathing
  • Development
  • Locus coeruleus
  • Mammals
  • Neonatal vs. adult
  • Nucleus
  • Pattern of breathing
  • Rat
  • Response

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Development of in vivo ventilatory and single chemosensitive neuron responses to hypercapnia in rats. / Stunden, C. E.; Filosa, J. A.; Garcia, A. J.; Dean, J. B.; Putnam, R. W.

In: Respiration Physiology, Vol. 127, No. 2-3, 18.08.2001, p. 135-155.

Research output: Contribution to journalArticle

Stunden, C. E. ; Filosa, J. A. ; Garcia, A. J. ; Dean, J. B. ; Putnam, R. W. / Development of in vivo ventilatory and single chemosensitive neuron responses to hypercapnia in rats. In: Respiration Physiology. 2001 ; Vol. 127, No. 2-3. pp. 135-155.
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