Dexamethasone receptor levels in palatal and lung fibroblasts of adult A/J and C57BL/6J mice

Relationship to glucocorticoid-induced cleft palate

R. Azziz, R. L. Ladda

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Glucocorticoid-induced cleft palate (CP) has been used as an animal model for hormonal teratogenesis. In mice, the susceptibility to glucocorticoid-induced CP varies with the strain, A/J being very sensitive and C57/BL6J relatively resistant. Studies in adult and embryonic murine tissues have attempted to correlate the number of glucocorticoid receptors and CP susceptibility, with conflicting results. The relative quantities of dexamethasone receptors were now studied in established palatal and lung fibroblast cell cultures obtained from adult C57 and A/J mice. A rapidly saturable, stable binding system was demonstrated. Scatchard plots were linear indicating a single class of high affinity receptors. The glucocorticoid receptor number ranged from 6.2 x 10-16 mole/μg prot to 8.6 x 10-16 mole/μg prot, while the K(D) varied from 1.0 x 1.0-8 M to 2.8 x 10-8 M. The differences in receptor characteristics between murine strains were not significant (p>0.05). The absence of a difference in receptor number between the two strains may reflect the limitation of fibroblast cell culture in assessing glucocorticoid binding in vivo. Alternatively, if a difference in palatal dexamethasone receptor levels between mice strains exists, it may occur only in the embryo.

Original languageEnglish (US)
Pages (from-to)388-391
Number of pages4
JournalCleft Palate Journal
Volume27
Issue number4
StatePublished - Nov 22 1990

Fingerprint

Cleft Palate
Inbred C57BL Mouse
Glucocorticoids
Fibroblasts
Glucocorticoid Receptors
Lung
Cell Culture Techniques
Teratogenesis
Embryonic Structures
Animal Models
dexamethasone receptor

ASJC Scopus subject areas

  • Surgery

Cite this

Dexamethasone receptor levels in palatal and lung fibroblasts of adult A/J and C57BL/6J mice : Relationship to glucocorticoid-induced cleft palate. / Azziz, R.; Ladda, R. L.

In: Cleft Palate Journal, Vol. 27, No. 4, 22.11.1990, p. 388-391.

Research output: Contribution to journalArticle

@article{06db1ef105e34cba9e95c63d20e67c87,
title = "Dexamethasone receptor levels in palatal and lung fibroblasts of adult A/J and C57BL/6J mice: Relationship to glucocorticoid-induced cleft palate",
abstract = "Glucocorticoid-induced cleft palate (CP) has been used as an animal model for hormonal teratogenesis. In mice, the susceptibility to glucocorticoid-induced CP varies with the strain, A/J being very sensitive and C57/BL6J relatively resistant. Studies in adult and embryonic murine tissues have attempted to correlate the number of glucocorticoid receptors and CP susceptibility, with conflicting results. The relative quantities of dexamethasone receptors were now studied in established palatal and lung fibroblast cell cultures obtained from adult C57 and A/J mice. A rapidly saturable, stable binding system was demonstrated. Scatchard plots were linear indicating a single class of high affinity receptors. The glucocorticoid receptor number ranged from 6.2 x 10-16 mole/μg prot to 8.6 x 10-16 mole/μg prot, while the K(D) varied from 1.0 x 1.0-8 M to 2.8 x 10-8 M. The differences in receptor characteristics between murine strains were not significant (p>0.05). The absence of a difference in receptor number between the two strains may reflect the limitation of fibroblast cell culture in assessing glucocorticoid binding in vivo. Alternatively, if a difference in palatal dexamethasone receptor levels between mice strains exists, it may occur only in the embryo.",
author = "R. Azziz and Ladda, {R. L.}",
year = "1990",
month = "11",
day = "22",
language = "English (US)",
volume = "27",
pages = "388--391",
journal = "Cleft Palate-Craniofacial Journal",
issn = "1055-6656",
publisher = "American Cleft Palate Craniofacial Association",
number = "4",

}

TY - JOUR

T1 - Dexamethasone receptor levels in palatal and lung fibroblasts of adult A/J and C57BL/6J mice

T2 - Relationship to glucocorticoid-induced cleft palate

AU - Azziz, R.

AU - Ladda, R. L.

PY - 1990/11/22

Y1 - 1990/11/22

N2 - Glucocorticoid-induced cleft palate (CP) has been used as an animal model for hormonal teratogenesis. In mice, the susceptibility to glucocorticoid-induced CP varies with the strain, A/J being very sensitive and C57/BL6J relatively resistant. Studies in adult and embryonic murine tissues have attempted to correlate the number of glucocorticoid receptors and CP susceptibility, with conflicting results. The relative quantities of dexamethasone receptors were now studied in established palatal and lung fibroblast cell cultures obtained from adult C57 and A/J mice. A rapidly saturable, stable binding system was demonstrated. Scatchard plots were linear indicating a single class of high affinity receptors. The glucocorticoid receptor number ranged from 6.2 x 10-16 mole/μg prot to 8.6 x 10-16 mole/μg prot, while the K(D) varied from 1.0 x 1.0-8 M to 2.8 x 10-8 M. The differences in receptor characteristics between murine strains were not significant (p>0.05). The absence of a difference in receptor number between the two strains may reflect the limitation of fibroblast cell culture in assessing glucocorticoid binding in vivo. Alternatively, if a difference in palatal dexamethasone receptor levels between mice strains exists, it may occur only in the embryo.

AB - Glucocorticoid-induced cleft palate (CP) has been used as an animal model for hormonal teratogenesis. In mice, the susceptibility to glucocorticoid-induced CP varies with the strain, A/J being very sensitive and C57/BL6J relatively resistant. Studies in adult and embryonic murine tissues have attempted to correlate the number of glucocorticoid receptors and CP susceptibility, with conflicting results. The relative quantities of dexamethasone receptors were now studied in established palatal and lung fibroblast cell cultures obtained from adult C57 and A/J mice. A rapidly saturable, stable binding system was demonstrated. Scatchard plots were linear indicating a single class of high affinity receptors. The glucocorticoid receptor number ranged from 6.2 x 10-16 mole/μg prot to 8.6 x 10-16 mole/μg prot, while the K(D) varied from 1.0 x 1.0-8 M to 2.8 x 10-8 M. The differences in receptor characteristics between murine strains were not significant (p>0.05). The absence of a difference in receptor number between the two strains may reflect the limitation of fibroblast cell culture in assessing glucocorticoid binding in vivo. Alternatively, if a difference in palatal dexamethasone receptor levels between mice strains exists, it may occur only in the embryo.

UR - http://www.scopus.com/inward/record.url?scp=0025088745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025088745&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 388

EP - 391

JO - Cleft Palate-Craniofacial Journal

JF - Cleft Palate-Craniofacial Journal

SN - 1055-6656

IS - 4

ER -