Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior

F. B. Rahmatpanah, S. Carstens, J. Guo, O. Sjahputera, K. H. Taylor, D. Duff, H. Shi, J. W. Davis, S. I. Hooshmand, R. Chitma-Matsiga, C. W. Caldwell

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Non-Hodgkin's lymphoma (NHL) is a group of malignancies of the immune system with variable clinical behaviors and diverse molecular features. Despite the progress made in classification of NHLs based on classical methods, molecular classifications are a work in progress. Toward this goal, we used an array-based technique called differential methylation hybridization (DMH) to study small B-cell lymphoma (SBCL) subtypes. A total of 43 genomic DMH experiments were performed. From these results, several statistical methods were used to generate a set of differentially methylated genes for further validation. Methylation of LHX2, POU3F3, HOXC10, NRP2, PRKCE, RAMP, MLLT2, NKX6.1, LRP1B and ARF4 was validated in cell lines and patient samples and demonstrated subtype-related preferential methylation patterns. For LHX2 and LRP1B, bisulfite sequencing, real-time reverse transcriptase-polymerase chain reaction and induction of gene expression following treatment with the demethylating agent, 5′-aza-2′-deoxycytidine, were confirmed. This new epigenetic information is helping to define molecular portraits of distinct subtypes of SBCL that are not recognized by current classification systems and provides valuable potential insights into the biology of these tumors.

Original languageEnglish (US)
Pages (from-to)1855-1862
Number of pages8
JournalLeukemia
Volume20
Issue number10
DOIs
StatePublished - Oct 2006
Externally publishedYes

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Rahmatpanah, F. B., Carstens, S., Guo, J., Sjahputera, O., Taylor, K. H., Duff, D., Shi, H., Davis, J. W., Hooshmand, S. I., Chitma-Matsiga, R., & Caldwell, C. W. (2006). Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior. Leukemia, 20(10), 1855-1862. https://doi.org/10.1038/sj.leu.2404345