Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior

F. B. Rahmatpanah, S. Carstens, J. Guo, O. Sjahputera, K. H. Taylor, D. Duff, Huidong Shi, J. W. Davis, S. I. Hooshmand, R. Chitma-Matsiga, C. W. Caldwell

Research output: Contribution to journalArticle

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Abstract

Non-Hodgkin's lymphoma (NHL) is a group of malignancies of the immune system with variable clinical behaviors and diverse molecular features. Despite the progress made in classification of NHLs based on classical methods, molecular classifications are a work in progress. Toward this goal, we used an array-based technique called differential methylation hybridization (DMH) to study small B-cell lymphoma (SBCL) subtypes. A total of 43 genomic DMH experiments were performed. From these results, several statistical methods were used to generate a set of differentially methylated genes for further validation. Methylation of LHX2, POU3F3, HOXC10, NRP2, PRKCE, RAMP, MLLT2, NKX6.1, LRP1B and ARF4 was validated in cell lines and patient samples and demonstrated subtype-related preferential methylation patterns. For LHX2 and LRP1B, bisulfite sequencing, real-time reverse transcriptase-polymerase chain reaction and induction of gene expression following treatment with the demethylating agent, 5′-aza-2′-deoxycytidine, were confirmed. This new epigenetic information is helping to define molecular portraits of distinct subtypes of SBCL that are not recognized by current classification systems and provides valuable potential insights into the biology of these tumors.

Original languageEnglish (US)
Pages (from-to)1855-1862
Number of pages8
JournalLeukemia
Volume20
Issue number10
DOIs
StatePublished - Jan 1 2006

Fingerprint

B-Cell Lymphoma
DNA Methylation
B-Cell Chronic Lymphocytic Leukemia
Methylation
decitabine
Architectural Accessibility
Reverse Transcriptase Polymerase Chain Reaction
Epigenomics
Non-Hodgkin's Lymphoma
Real-Time Polymerase Chain Reaction
Immune System
Neoplasms
Gene Expression
Cell Line
Genes
Therapeutics

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Rahmatpanah, F. B., Carstens, S., Guo, J., Sjahputera, O., Taylor, K. H., Duff, D., ... Caldwell, C. W. (2006). Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior. Leukemia, 20(10), 1855-1862. https://doi.org/10.1038/sj.leu.2404345

Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior. / Rahmatpanah, F. B.; Carstens, S.; Guo, J.; Sjahputera, O.; Taylor, K. H.; Duff, D.; Shi, Huidong; Davis, J. W.; Hooshmand, S. I.; Chitma-Matsiga, R.; Caldwell, C. W.

In: Leukemia, Vol. 20, No. 10, 01.01.2006, p. 1855-1862.

Research output: Contribution to journalArticle

Rahmatpanah, FB, Carstens, S, Guo, J, Sjahputera, O, Taylor, KH, Duff, D, Shi, H, Davis, JW, Hooshmand, SI, Chitma-Matsiga, R & Caldwell, CW 2006, 'Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior', Leukemia, vol. 20, no. 10, pp. 1855-1862. https://doi.org/10.1038/sj.leu.2404345
Rahmatpanah FB, Carstens S, Guo J, Sjahputera O, Taylor KH, Duff D et al. Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior. Leukemia. 2006 Jan 1;20(10):1855-1862. https://doi.org/10.1038/sj.leu.2404345
Rahmatpanah, F. B. ; Carstens, S. ; Guo, J. ; Sjahputera, O. ; Taylor, K. H. ; Duff, D. ; Shi, Huidong ; Davis, J. W. ; Hooshmand, S. I. ; Chitma-Matsiga, R. ; Caldwell, C. W. / Differential DNA methylation patterns of small B-cell lymphoma subclasses with different clinical behavior. In: Leukemia. 2006 ; Vol. 20, No. 10. pp. 1855-1862.
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