Differential expression of alpha smooth muscle cell actin in human fibroblasts isolated from intraperitoneal adhesions and normal peritoneal tissues

Ghassan M. Saed, Michael P. Diamond

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24 Citations (Scopus)

Abstract

To determine whether α smooth muscle cell actin (αSMCA) is expressed in human fibroblasts isolated from normal peritoneal and adhesion tissues. Prospective, experimental study. University medical center. Five patients undergoing laparotomy for adhesiolysis for pelvic pain. Primary culture of fibroblasts from both peritoneum and adhesion tissues. Immunohistochemistry techniques were used to determine whether αSMCA was present in normal peritoneal and adhesion fibroblasts from the same patients. Cultured fibroblasts from all tissues were also fixed on slides and stained with αSMCA monoclonal antibody labeled with immunofluorescence. Alpha SMCA protein was present in very low levels in the cytoplasm of normal peritoneal fibroblasts from all five subjects, as compared with high levels present in adhesion fibroblasts from the same patients. Adhesion fibroblasts develop a specific phenotype, an adhesion phenotype, which in part is characterized by the over-expression of αSMCA. The over-expression of αSMCA in adhesion fibroblasts indicates a possible response to peritoneal injury. Regulation of αSMCA might alter peritoneal healing and might provide the opportunity to reduce postoperative adhesion development.

Original languageEnglish (US)
Pages (from-to)1188-1192
Number of pages5
JournalFertility and sterility
Volume82
Issue numberSUPPL. 3
DOIs
StatePublished - Oct 1 2004

Fingerprint

Smooth Muscle Myocytes
Actins
Fibroblasts
Tissue Adhesions
Phenotype
Pelvic Pain
Peritoneum
Laparotomy
Fluorescent Antibody Technique
Cytoplasm
Immunohistochemistry
Monoclonal Antibodies
Prospective Studies
Wounds and Injuries
Proteins

Keywords

  • Fibroblasts
  • adhesion
  • immunohistochemistry
  • peritoneum
  • αSMCA

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

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abstract = "To determine whether α smooth muscle cell actin (αSMCA) is expressed in human fibroblasts isolated from normal peritoneal and adhesion tissues. Prospective, experimental study. University medical center. Five patients undergoing laparotomy for adhesiolysis for pelvic pain. Primary culture of fibroblasts from both peritoneum and adhesion tissues. Immunohistochemistry techniques were used to determine whether αSMCA was present in normal peritoneal and adhesion fibroblasts from the same patients. Cultured fibroblasts from all tissues were also fixed on slides and stained with αSMCA monoclonal antibody labeled with immunofluorescence. Alpha SMCA protein was present in very low levels in the cytoplasm of normal peritoneal fibroblasts from all five subjects, as compared with high levels present in adhesion fibroblasts from the same patients. Adhesion fibroblasts develop a specific phenotype, an adhesion phenotype, which in part is characterized by the over-expression of αSMCA. The over-expression of αSMCA in adhesion fibroblasts indicates a possible response to peritoneal injury. Regulation of αSMCA might alter peritoneal healing and might provide the opportunity to reduce postoperative adhesion development.",
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AU - Diamond, Michael P.

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N2 - To determine whether α smooth muscle cell actin (αSMCA) is expressed in human fibroblasts isolated from normal peritoneal and adhesion tissues. Prospective, experimental study. University medical center. Five patients undergoing laparotomy for adhesiolysis for pelvic pain. Primary culture of fibroblasts from both peritoneum and adhesion tissues. Immunohistochemistry techniques were used to determine whether αSMCA was present in normal peritoneal and adhesion fibroblasts from the same patients. Cultured fibroblasts from all tissues were also fixed on slides and stained with αSMCA monoclonal antibody labeled with immunofluorescence. Alpha SMCA protein was present in very low levels in the cytoplasm of normal peritoneal fibroblasts from all five subjects, as compared with high levels present in adhesion fibroblasts from the same patients. Adhesion fibroblasts develop a specific phenotype, an adhesion phenotype, which in part is characterized by the over-expression of αSMCA. The over-expression of αSMCA in adhesion fibroblasts indicates a possible response to peritoneal injury. Regulation of αSMCA might alter peritoneal healing and might provide the opportunity to reduce postoperative adhesion development.

AB - To determine whether α smooth muscle cell actin (αSMCA) is expressed in human fibroblasts isolated from normal peritoneal and adhesion tissues. Prospective, experimental study. University medical center. Five patients undergoing laparotomy for adhesiolysis for pelvic pain. Primary culture of fibroblasts from both peritoneum and adhesion tissues. Immunohistochemistry techniques were used to determine whether αSMCA was present in normal peritoneal and adhesion fibroblasts from the same patients. Cultured fibroblasts from all tissues were also fixed on slides and stained with αSMCA monoclonal antibody labeled with immunofluorescence. Alpha SMCA protein was present in very low levels in the cytoplasm of normal peritoneal fibroblasts from all five subjects, as compared with high levels present in adhesion fibroblasts from the same patients. Adhesion fibroblasts develop a specific phenotype, an adhesion phenotype, which in part is characterized by the over-expression of αSMCA. The over-expression of αSMCA in adhesion fibroblasts indicates a possible response to peritoneal injury. Regulation of αSMCA might alter peritoneal healing and might provide the opportunity to reduce postoperative adhesion development.

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