Differential expression of antiapoptotic gene BAG-1 in human breast normal and cancer cell lines and tissues

Xiaolong Yang, Yawei Hao, Zhihu Ding, Alan Pater, Shou Ching Tang

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

BAG-1 is an antiapoptotic protein that binds to and enhances the antiapoptotic activity of Bcl-2. It binds several growth factor and hormone receptors and modulates their function. BAG-1 was also shown recently to be expressed as four protein isoforms, p50, p46, p33, and p29, through alternative translation initiation. Although many apoptosis-associated genes have been linked to oncogenesis of human breast cancer, the role of BAG-1 has not been fully elucidated. In this study, we examined the expression of BAG- 1 RNA or protein isoforms and its interacting antiapoptotic proteins, Bcl-2 and Bcl-X(L), in breast normal and tumor cell lines and tissues by Northern or Western blot analysis. We provide convincing evidence that both BAG-1 RNA and protein are overexpressed in human breast cancer cell lines. More importantly, we found that the expression of two isoforms of BAG-1, p46 and p33, was also much higher in breast primary tumors. The expression of Bcl-2 and Bcl-X(L) correlated with that of BAG-1 in breast normal and carcinoma cell lines but not tissues. Our study suggests that BAG-1 isoforms may serve as a molecular marker, independent of Bcl-2 and Bcl-X(L), for human breast cancer.

Original languageEnglish (US)
Pages (from-to)1816-1822
Number of pages7
JournalClinical Cancer Research
Volume5
Issue number7
StatePublished - Jul 1 1999

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Breast Neoplasms
Gene Expression
Cell Line
Protein Isoforms
RNA Isoforms
Somatotropin Receptors
Growth Factor Receptors
Tumor Cell Line
Northern Blotting
Carcinogenesis
Proteins
Western Blotting
RNA
Apoptosis
Genes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Differential expression of antiapoptotic gene BAG-1 in human breast normal and cancer cell lines and tissues. / Yang, Xiaolong; Hao, Yawei; Ding, Zhihu; Pater, Alan; Tang, Shou Ching.

In: Clinical Cancer Research, Vol. 5, No. 7, 01.07.1999, p. 1816-1822.

Research output: Contribution to journalArticle

Yang, Xiaolong ; Hao, Yawei ; Ding, Zhihu ; Pater, Alan ; Tang, Shou Ching. / Differential expression of antiapoptotic gene BAG-1 in human breast normal and cancer cell lines and tissues. In: Clinical Cancer Research. 1999 ; Vol. 5, No. 7. pp. 1816-1822.
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