Dimensional changes of demineralized human dentine during preparation for scanning electron microscopy

R. M. Carvalho, M. Yoshiyama, P. D. Brewer, David Henry Pashley

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Dentine rods measuring approx. 0.7 × 0.7 × 5.0 mm were prepared from the crowns of extracted human third molars. The specimens were demineralized in 0.5 M EDTA (pH 7.0) for 3 days and their volume measured with a digital micrometer under a dissecting microscope. The specimens were randomly assigned to experimental groups and were chemically dehydrated in acetone. Next they were dried using either hexamethyldisilazane, Peldri II, or critical-point drying techniques. The dimensions of the specimens were measured again after each step and the changes in volume were expressed as a percentage of the original demineralized volume. The effects of fixing the specimens in 10% buffered formalin before dehydration with acetone were also investigated for every drying procedure. Dehydration in acetone caused a small but significant reduction in the volume of demineralized formalin-fixed specimens but unfixed specimens did not change significantly. In general, all three drying procedures caused some shrinkage in demineralized dentine specimens. Unfixed specimens exhibited a volumetric shrinkage of approx. 15-20% after drying with any of the methods. Fixed specimens shrank more than unfixed specimens after drying (25-35%). Regardless of the drying technique, the specimens shrank a further 10-20% when measured in the vacuum chamber of the scanning electron microscope. Among the three drying techniques employed, hexamethyldisilazane seems to be a very useful alternative to critical-point drying for the preparation of dentine specimens for scanning electron microscopy.

Original languageEnglish (US)
Pages (from-to)379-386
Number of pages8
JournalArchives of Oral Biology
Volume41
Issue number4
DOIs
StatePublished - Jan 1 1996

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Dentin
Acetone
Electron Scanning Microscopy
Dehydration
Formaldehyde
Third Molar
Vacuum
Crowns
Edetic Acid
Electrons
hexamethylsilazane

Keywords

  • Dentine
  • Shrinkage
  • Tissue preparation

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Dimensional changes of demineralized human dentine during preparation for scanning electron microscopy. / Carvalho, R. M.; Yoshiyama, M.; Brewer, P. D.; Pashley, David Henry.

In: Archives of Oral Biology, Vol. 41, No. 4, 01.01.1996, p. 379-386.

Research output: Contribution to journalArticle

Carvalho, R. M. ; Yoshiyama, M. ; Brewer, P. D. ; Pashley, David Henry. / Dimensional changes of demineralized human dentine during preparation for scanning electron microscopy. In: Archives of Oral Biology. 1996 ; Vol. 41, No. 4. pp. 379-386.
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