Discordance in deoxyribonucleic acid analysis of fetus and trophoblast

William J. Butler; Charles E. Schwartz; Suzanne M. Sauer; John T. Wilson; Paul G McDonough

doi:10.1016/0002-9378(88)90046-4

Discordance in deoxyribonucleic acid analysis of fetus and trophoblast

William J. Butler, Charles E. Schwartz, Suzanne M. Sauer, John T. Wilson, Paul G McDonough

Physiology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Molecular analysis of deoxyribonucleic acid extracted from trophoblast has become an important component of modern prenatal diagnosis. Discrepancies between trophoblast and fetal tissue in both chromosomal and metabolic analysis have been reported. We studied 50 paired fetal and trophoblast deoxyribonucleic acid specimens with a highly polymorphic probe for repetitive "minisatellite" regions of deoxyribonucleic acid that may be areas of high recombination. Four pairs showed persistent variations in bands consistent with differences in fetal and trophoblast deoxyribonucleic acid. Discordance in trophoblast deoxyribonucleic acid as a reflection of fetal genotype may be secondary to mitotic recombination or sister chromatid exchange in the trophoblast, and the possiility of discordance must be considered in interpreting the results of prenatal diagnostic testing on trophoblast specimens.

Original language	English (US)
Pages (from-to)	642-645
Number of pages	4
Journal	American journal of obstetrics and gynecology
Volume	158
Issue number	3 PART 1
DOIs	https://doi.org/10.1016/0002-9378(88)90046-4
State	Published - Mar 1988

Keywords

Prenatal diagnosis
deoxyribonucleic acid analysis
trophoblast

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/0002-9378(88)90046-4

Cite this

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title = "Discordance in deoxyribonucleic acid analysis of fetus and trophoblast",

abstract = "Molecular analysis of deoxyribonucleic acid extracted from trophoblast has become an important component of modern prenatal diagnosis. Discrepancies between trophoblast and fetal tissue in both chromosomal and metabolic analysis have been reported. We studied 50 paired fetal and trophoblast deoxyribonucleic acid specimens with a highly polymorphic probe for repetitive {"}minisatellite{"} regions of deoxyribonucleic acid that may be areas of high recombination. Four pairs showed persistent variations in bands consistent with differences in fetal and trophoblast deoxyribonucleic acid. Discordance in trophoblast deoxyribonucleic acid as a reflection of fetal genotype may be secondary to mitotic recombination or sister chromatid exchange in the trophoblast, and the possiility of discordance must be considered in interpreting the results of prenatal diagnostic testing on trophoblast specimens.",

keywords = "Prenatal diagnosis, deoxyribonucleic acid analysis, trophoblast",

author = "Butler, {William J.} and Schwartz, {Charles E.} and Sauer, {Suzanne M.} and Wilson, {John T.} and McDonough, {Paul G}",

note = "Funding Information: From the Section of Reproductive Endocrinology, Infertility and Ge-netirs, Department of Obstetrics and G_vnecology{"} and the Depart-ment of Oral Biology, School of Dentistry, Medical College of Geor-gia, and the Recombinant DNA Laboratory, Greenwood Genetics Center. · This work was supported in part by grant1 from the Self Foundation and the South Carolina Department of Mental Retardation. Received for publication August JO, 1987; revised September JO, 1987; accepted October 19, 1987. Reprint requests: William]. Butler, MD, Director, Reproductive En-docrinology and Reproductive Genetics, Department of Obstetrics and Gynecology, Albany Medical College, Albany, NY 12208.",

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PY - 1988/3

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N2 - Molecular analysis of deoxyribonucleic acid extracted from trophoblast has become an important component of modern prenatal diagnosis. Discrepancies between trophoblast and fetal tissue in both chromosomal and metabolic analysis have been reported. We studied 50 paired fetal and trophoblast deoxyribonucleic acid specimens with a highly polymorphic probe for repetitive "minisatellite" regions of deoxyribonucleic acid that may be areas of high recombination. Four pairs showed persistent variations in bands consistent with differences in fetal and trophoblast deoxyribonucleic acid. Discordance in trophoblast deoxyribonucleic acid as a reflection of fetal genotype may be secondary to mitotic recombination or sister chromatid exchange in the trophoblast, and the possiility of discordance must be considered in interpreting the results of prenatal diagnostic testing on trophoblast specimens.

AB - Molecular analysis of deoxyribonucleic acid extracted from trophoblast has become an important component of modern prenatal diagnosis. Discrepancies between trophoblast and fetal tissue in both chromosomal and metabolic analysis have been reported. We studied 50 paired fetal and trophoblast deoxyribonucleic acid specimens with a highly polymorphic probe for repetitive "minisatellite" regions of deoxyribonucleic acid that may be areas of high recombination. Four pairs showed persistent variations in bands consistent with differences in fetal and trophoblast deoxyribonucleic acid. Discordance in trophoblast deoxyribonucleic acid as a reflection of fetal genotype may be secondary to mitotic recombination or sister chromatid exchange in the trophoblast, and the possiility of discordance must be considered in interpreting the results of prenatal diagnostic testing on trophoblast specimens.

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Discordance in deoxyribonucleic acid analysis of fetus and trophoblast

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