Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow

Xutong Sun, Shruti Sharma, Sohrab Fratz, Sanjiv Kumar, Ruslan Rafikov, Saurabh Aggarwal, Olga Rafikova, Qing Lu, Tantiana Burns, Sridevi Dasarathy, Johnny Wright, Christian Schreiber, Monique Radman, Jeffrey R. Fineman, Stephen Matthew Black

Research output: Contribution to journalReview article

18 Citations (Scopus)

Abstract

Aims: The mitochondrial dysfunction in our lamb model of congenital heart disease with increased pulmonary blood flow (PBF) (Shunt) is associated with disrupted carnitine metabolism. Our recent studies have also shown that asymmetric dimethylarginine (ADMA) levels are increased in Shunt lambs and ADMA increases the nitration of mitochondrial proteins in lamb pulmonary arterial endothelial cells (PAEC) in a nitric oxide synthase (NOS)-dependent manner. Thus, we determined whether there was a mechanistic link between endothelial nitric oxide synthase (eNOS), ADMA, and the disruption of carnitine homeostasis in PAEC. Results: Exposure of PAEC to ADMA induced the redistribution of eNOS to the mitochondria, resulting in an increase in carnitine acetyl transferase (CrAT) nitration and decreased CrAT activity. The resulting increase in acyl-carnitine levels resulted in mitochondrial dysfunction and the disruption of mitochondrial bioenergetics. Since the addition of l-arginine prevented these pathologic changes, we examined the effect of l-arginine supplementation on carnitine homeostasis, mitochondrial function, and nitric oxide (NO) signaling in Shunt lambs. We found that the treatment of Shunt lambs with l-arginine prevented the ADMA-mediated mitochondrial redistribution of eNOS, the nitration-mediated inhibition of CrAT, and maintained carnitine homeostasis. In turn, adenosine-5′-triphosphate levels and eNOS/heat shock protein 90 interactions were preserved, and this decreased NOS uncoupling and enhanced NO generation. Innovation: Our data link alterations in cellular l-arginine metabolism with the disruption of mitochondrial bioenergetics and implicate altered carnitine homeostasis as a key player in this process. Conclusion: l-arginine supplementation may be a useful therapy to prevent the mitochondrial dysfunction involved in the pulmonary vascular alterations secondary to increased PBF.

Original languageEnglish (US)
Pages (from-to)1739-1752
Number of pages14
JournalAntioxidants and Redox Signaling
Volume18
Issue number14
DOIs
StatePublished - May 10 2013

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Carnitine
Endothelial cells
Energy Metabolism
Blood
Endothelial Cells
Nitric Oxide Synthase Type III
Arginine
Acetylcarnitine
Nitration
Lung
Homeostasis
Transferases
Metabolism
Nitric Oxide Synthase
Nitric Oxide
HSP90 Heat-Shock Proteins
Mitochondria
Mitochondrial Proteins
Adenosine
Blood Vessels

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow. / Sun, Xutong; Sharma, Shruti; Fratz, Sohrab; Kumar, Sanjiv; Rafikov, Ruslan; Aggarwal, Saurabh; Rafikova, Olga; Lu, Qing; Burns, Tantiana; Dasarathy, Sridevi; Wright, Johnny; Schreiber, Christian; Radman, Monique; Fineman, Jeffrey R.; Black, Stephen Matthew.

In: Antioxidants and Redox Signaling, Vol. 18, No. 14, 10.05.2013, p. 1739-1752.

Research output: Contribution to journalReview article

Sun, X, Sharma, S, Fratz, S, Kumar, S, Rafikov, R, Aggarwal, S, Rafikova, O, Lu, Q, Burns, T, Dasarathy, S, Wright, J, Schreiber, C, Radman, M, Fineman, JR & Black, SM 2013, 'Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow', Antioxidants and Redox Signaling, vol. 18, no. 14, pp. 1739-1752. https://doi.org/10.1089/ars.2012.4806
Sun, Xutong ; Sharma, Shruti ; Fratz, Sohrab ; Kumar, Sanjiv ; Rafikov, Ruslan ; Aggarwal, Saurabh ; Rafikova, Olga ; Lu, Qing ; Burns, Tantiana ; Dasarathy, Sridevi ; Wright, Johnny ; Schreiber, Christian ; Radman, Monique ; Fineman, Jeffrey R. ; Black, Stephen Matthew. / Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow. In: Antioxidants and Redox Signaling. 2013 ; Vol. 18, No. 14. pp. 1739-1752.
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abstract = "Aims: The mitochondrial dysfunction in our lamb model of congenital heart disease with increased pulmonary blood flow (PBF) (Shunt) is associated with disrupted carnitine metabolism. Our recent studies have also shown that asymmetric dimethylarginine (ADMA) levels are increased in Shunt lambs and ADMA increases the nitration of mitochondrial proteins in lamb pulmonary arterial endothelial cells (PAEC) in a nitric oxide synthase (NOS)-dependent manner. Thus, we determined whether there was a mechanistic link between endothelial nitric oxide synthase (eNOS), ADMA, and the disruption of carnitine homeostasis in PAEC. Results: Exposure of PAEC to ADMA induced the redistribution of eNOS to the mitochondria, resulting in an increase in carnitine acetyl transferase (CrAT) nitration and decreased CrAT activity. The resulting increase in acyl-carnitine levels resulted in mitochondrial dysfunction and the disruption of mitochondrial bioenergetics. Since the addition of l-arginine prevented these pathologic changes, we examined the effect of l-arginine supplementation on carnitine homeostasis, mitochondrial function, and nitric oxide (NO) signaling in Shunt lambs. We found that the treatment of Shunt lambs with l-arginine prevented the ADMA-mediated mitochondrial redistribution of eNOS, the nitration-mediated inhibition of CrAT, and maintained carnitine homeostasis. In turn, adenosine-5′-triphosphate levels and eNOS/heat shock protein 90 interactions were preserved, and this decreased NOS uncoupling and enhanced NO generation. Innovation: Our data link alterations in cellular l-arginine metabolism with the disruption of mitochondrial bioenergetics and implicate altered carnitine homeostasis as a key player in this process. Conclusion: l-arginine supplementation may be a useful therapy to prevent the mitochondrial dysfunction involved in the pulmonary vascular alterations secondary to increased PBF.",
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T1 - Disruption of endothelial cell mitochondrial bioenergetics in lambs with increased pulmonary blood flow

AU - Sun, Xutong

AU - Sharma, Shruti

AU - Fratz, Sohrab

AU - Kumar, Sanjiv

AU - Rafikov, Ruslan

AU - Aggarwal, Saurabh

AU - Rafikova, Olga

AU - Lu, Qing

AU - Burns, Tantiana

AU - Dasarathy, Sridevi

AU - Wright, Johnny

AU - Schreiber, Christian

AU - Radman, Monique

AU - Fineman, Jeffrey R.

AU - Black, Stephen Matthew

PY - 2013/5/10

Y1 - 2013/5/10

N2 - Aims: The mitochondrial dysfunction in our lamb model of congenital heart disease with increased pulmonary blood flow (PBF) (Shunt) is associated with disrupted carnitine metabolism. Our recent studies have also shown that asymmetric dimethylarginine (ADMA) levels are increased in Shunt lambs and ADMA increases the nitration of mitochondrial proteins in lamb pulmonary arterial endothelial cells (PAEC) in a nitric oxide synthase (NOS)-dependent manner. Thus, we determined whether there was a mechanistic link between endothelial nitric oxide synthase (eNOS), ADMA, and the disruption of carnitine homeostasis in PAEC. Results: Exposure of PAEC to ADMA induced the redistribution of eNOS to the mitochondria, resulting in an increase in carnitine acetyl transferase (CrAT) nitration and decreased CrAT activity. The resulting increase in acyl-carnitine levels resulted in mitochondrial dysfunction and the disruption of mitochondrial bioenergetics. Since the addition of l-arginine prevented these pathologic changes, we examined the effect of l-arginine supplementation on carnitine homeostasis, mitochondrial function, and nitric oxide (NO) signaling in Shunt lambs. We found that the treatment of Shunt lambs with l-arginine prevented the ADMA-mediated mitochondrial redistribution of eNOS, the nitration-mediated inhibition of CrAT, and maintained carnitine homeostasis. In turn, adenosine-5′-triphosphate levels and eNOS/heat shock protein 90 interactions were preserved, and this decreased NOS uncoupling and enhanced NO generation. Innovation: Our data link alterations in cellular l-arginine metabolism with the disruption of mitochondrial bioenergetics and implicate altered carnitine homeostasis as a key player in this process. Conclusion: l-arginine supplementation may be a useful therapy to prevent the mitochondrial dysfunction involved in the pulmonary vascular alterations secondary to increased PBF.

AB - Aims: The mitochondrial dysfunction in our lamb model of congenital heart disease with increased pulmonary blood flow (PBF) (Shunt) is associated with disrupted carnitine metabolism. Our recent studies have also shown that asymmetric dimethylarginine (ADMA) levels are increased in Shunt lambs and ADMA increases the nitration of mitochondrial proteins in lamb pulmonary arterial endothelial cells (PAEC) in a nitric oxide synthase (NOS)-dependent manner. Thus, we determined whether there was a mechanistic link between endothelial nitric oxide synthase (eNOS), ADMA, and the disruption of carnitine homeostasis in PAEC. Results: Exposure of PAEC to ADMA induced the redistribution of eNOS to the mitochondria, resulting in an increase in carnitine acetyl transferase (CrAT) nitration and decreased CrAT activity. The resulting increase in acyl-carnitine levels resulted in mitochondrial dysfunction and the disruption of mitochondrial bioenergetics. Since the addition of l-arginine prevented these pathologic changes, we examined the effect of l-arginine supplementation on carnitine homeostasis, mitochondrial function, and nitric oxide (NO) signaling in Shunt lambs. We found that the treatment of Shunt lambs with l-arginine prevented the ADMA-mediated mitochondrial redistribution of eNOS, the nitration-mediated inhibition of CrAT, and maintained carnitine homeostasis. In turn, adenosine-5′-triphosphate levels and eNOS/heat shock protein 90 interactions were preserved, and this decreased NOS uncoupling and enhanced NO generation. Innovation: Our data link alterations in cellular l-arginine metabolism with the disruption of mitochondrial bioenergetics and implicate altered carnitine homeostasis as a key player in this process. Conclusion: l-arginine supplementation may be a useful therapy to prevent the mitochondrial dysfunction involved in the pulmonary vascular alterations secondary to increased PBF.

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