DNA-dependent protein phosphorylation activity in Xenopus is coupled to a Ku-like protein

Jyotshnabala Kanungo, Richard S Cameron, Yoshihiko Takeda, John A. Hardin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

DNA-dependent protein kinase (DNA-PK) is a nuclear enzyme and functions as a serine/threonine kinase that has been well characterized in both the human and the mouse. The regulatory subunit of DNA-PK is the Ku autoantigen. To demonstrate that a Ku-like protein is present in Xenopus oocytes, we used immunoprecipitation analysis with a monoclonal antibody raised against human Ku antigen and autoimmune serum containing anti-Ku antibodies. Metabolic labeling studies indicate that the Ku-like protein is synthesized mainly in late vitellogenic oocytes. By using a specific peptide substrate for DNA-PK, we demonstrate the activity of a DNA-dependent protein kinase in oocyte extracts. The kinase activity requires the Ku-like protein, since extracts depleted of Ku protein by immunoadsorption with human anti-Ku antibodies fail to demonstrate the DNA-dependent phosphorylation activity. The increased enzyme activity in vitellogenic oocytes may be correlated to the increased levels of Ku protein observed in these oocytes compared to the pre- and early vitellogenic oocytes.

Original languageEnglish (US)
Pages (from-to)147-152
Number of pages6
JournalBiological Bulletin
Volume193
Issue number2
DOIs
StatePublished - Jan 1 1997

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protein phosphorylation
Xenopus
DNA-Activated Protein Kinase
oocytes
Oocytes
Phosphorylation
protein kinases
DNA
Proteins
proteins
phosphotransferases (kinases)
Anti-Idiotypic Antibodies
autoantigens
antibodies
extracts
Protein-Serine-Threonine Kinases
threonine
serine
Enzymes
Ku Autoantigen

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

DNA-dependent protein phosphorylation activity in Xenopus is coupled to a Ku-like protein. / Kanungo, Jyotshnabala; Cameron, Richard S; Takeda, Yoshihiko; Hardin, John A.

In: Biological Bulletin, Vol. 193, No. 2, 01.01.1997, p. 147-152.

Research output: Contribution to journalArticle

Kanungo, Jyotshnabala ; Cameron, Richard S ; Takeda, Yoshihiko ; Hardin, John A. / DNA-dependent protein phosphorylation activity in Xenopus is coupled to a Ku-like protein. In: Biological Bulletin. 1997 ; Vol. 193, No. 2. pp. 147-152.
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