DNA from various tissues of far eastern larches and its applicability for RAPD assay

I. Yu Sazonova, M. M. Kozyrenko, E. V. Artyukova, G. D. Reunova, Y. N. Zhuravlev

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Abstract

We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

Original languageEnglish (US)
Pages (from-to)196-201
Number of pages6
JournalBiology Bulletin
Volume28
Issue number2
DOIs
Publication statusPublished - Jan 1 2001

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sazonova, I. Y., Kozyrenko, M. M., Artyukova, E. V., Reunova, G. D., & Zhuravlev, Y. N. (2001). DNA from various tissues of far eastern larches and its applicability for RAPD assay. Biology Bulletin, 28(2), 196-201. https://doi.org/10.1023/A:1009479319111