DNA from various tissues of far eastern larches and its applicability for RAPD assay

Irina Yurievna Sazonova, M. M. Kozyrenko, E. V. Artyukova, G. D. Reunova, Y. N. Zhuravlev

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We studied applicability of RAPD assay for investigating genetic variability of far eastern larches. Techniques to isolate DNA from larch vegetative organs (needles, young shoots and stems) and megagametophytes were selected, and conditions of polymerase chain reaction were optimized. Fifty out of 84 random primers proved efficient, and 22 and 12 primers initiated weak and no synthesis, respectively. The tested primers worked efficiently on the DNA isolated from both diploid and haploid tissues.

Original languageEnglish (US)
Pages (from-to)196-201
Number of pages6
JournalBiology Bulletin
Volume28
Issue number2
DOIs
StatePublished - Jan 1 2001

Fingerprint

Assays
Larix
Tissue
Ovule
Haploidy
Polymerase chain reaction
DNA
assays
Diploidy
haploidy
Needles
diploidy
polymerase chain reaction
Polymerase Chain Reaction
genetic variation
stems
synthesis
shoots
tissues
methodology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Sazonova, I. Y., Kozyrenko, M. M., Artyukova, E. V., Reunova, G. D., & Zhuravlev, Y. N. (2001). DNA from various tissues of far eastern larches and its applicability for RAPD assay. Biology Bulletin, 28(2), 196-201. https://doi.org/10.1023/A:1009479319111

DNA from various tissues of far eastern larches and its applicability for RAPD assay. / Sazonova, Irina Yurievna; Kozyrenko, M. M.; Artyukova, E. V.; Reunova, G. D.; Zhuravlev, Y. N.

In: Biology Bulletin, Vol. 28, No. 2, 01.01.2001, p. 196-201.

Research output: Contribution to journalArticle

Sazonova, IY, Kozyrenko, MM, Artyukova, EV, Reunova, GD & Zhuravlev, YN 2001, 'DNA from various tissues of far eastern larches and its applicability for RAPD assay', Biology Bulletin, vol. 28, no. 2, pp. 196-201. https://doi.org/10.1023/A:1009479319111
Sazonova IY, Kozyrenko MM, Artyukova EV, Reunova GD, Zhuravlev YN. DNA from various tissues of far eastern larches and its applicability for RAPD assay. Biology Bulletin. 2001 Jan 1;28(2):196-201. https://doi.org/10.1023/A:1009479319111
Sazonova, Irina Yurievna ; Kozyrenko, M. M. ; Artyukova, E. V. ; Reunova, G. D. ; Zhuravlev, Y. N. / DNA from various tissues of far eastern larches and its applicability for RAPD assay. In: Biology Bulletin. 2001 ; Vol. 28, No. 2. pp. 196-201.
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