Does β3-adrenoreceptor blockade attenuate acute exercise-induced reductions in leptin mRNA?

S. Brooke Bramlett, Jun Zhou, Ruth Babette Harris, Stephen L. Hendry, Trudy L. Witt, Jeffrey J. Zachwieja

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We investigated the effect of a single bout of exercise on leptin mRNA levels in rat white adipose tissue. Male Sprague-Dawley rats were randomly assigned to an exercise or control group. Acute exercise was performed on a rodent treadmill and was carried out to exhaustion, lasting an average of 85.5 ± 1.5 min. At the end of exercise, soleus muscle and liver glycogen were reduced by 88% (P < 0.001). Acutely exercised animals had lower (P < 0.05) leptin mRNA levels in retroperitoneal but not epididymal fat, and this was independent of fat pad weight. To test the hypothesis that β3- adrenergic-receptor stimulation was involved in the downregulation of leptin mRNA in retroperitoneal fat, a second experiment was performed in which rats were randomized into one of four groups: control, control + β3-antagonist, exercise, and exercise + β3-antagonist. A highly selective β3-antagonist (SR-59230A) or vehicle was given by gavage 30 min before exercise or control experiment. Exercise consisted of 55 min of treadmill running, sufficient to reduce liver and muscle glycogen by 70 and 80%, respectively (both P < 0.0001). Again, acute exercise reduced leptin mRNA in retroperitoneal fat (exercise vs. control; P < 0.05), but β3-antagonism blocked this effect (exercise + β3-antagonist vs. control + β3-antagonist; P = 0.42). Unexpectedly, exercise increased serum leptin. This would be consistent with the idea that there are releasable, preformed pools of leptin within adipocytes. We conclude that β3-receptor stimulation is a mechanism by which acute exercise downregulates retroperitoneal adipose tissue leptin mRNA in vivo.

Original languageEnglish (US)
Pages (from-to)1678-1683
Number of pages6
JournalJournal of Applied Physiology
Volume87
Issue number5
StatePublished - Nov 1 1999
Externally publishedYes

Fingerprint

Leptin
Exercise
Messenger RNA
Intra-Abdominal Fat
Liver Glycogen
Down-Regulation
White Adipose Tissue
Control Groups
Adipocytes
Running
Adrenergic Receptors
Sprague Dawley Rats
Adipose Tissue
Rodentia
Skeletal Muscle
Fats
Weights and Measures
Muscles
Serum

Keywords

  • Adipose tissue
  • Energy expenditure
  • Insulin
  • Ob gene
  • Sympathetic nervous system

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Bramlett, S. B., Zhou, J., Harris, R. B., Hendry, S. L., Witt, T. L., & Zachwieja, J. J. (1999). Does β3-adrenoreceptor blockade attenuate acute exercise-induced reductions in leptin mRNA? Journal of Applied Physiology, 87(5), 1678-1683.

Does β3-adrenoreceptor blockade attenuate acute exercise-induced reductions in leptin mRNA? / Bramlett, S. Brooke; Zhou, Jun; Harris, Ruth Babette; Hendry, Stephen L.; Witt, Trudy L.; Zachwieja, Jeffrey J.

In: Journal of Applied Physiology, Vol. 87, No. 5, 01.11.1999, p. 1678-1683.

Research output: Contribution to journalArticle

Bramlett, SB, Zhou, J, Harris, RB, Hendry, SL, Witt, TL & Zachwieja, JJ 1999, 'Does β3-adrenoreceptor blockade attenuate acute exercise-induced reductions in leptin mRNA?', Journal of Applied Physiology, vol. 87, no. 5, pp. 1678-1683.
Bramlett, S. Brooke ; Zhou, Jun ; Harris, Ruth Babette ; Hendry, Stephen L. ; Witt, Trudy L. ; Zachwieja, Jeffrey J. / Does β3-adrenoreceptor blockade attenuate acute exercise-induced reductions in leptin mRNA?. In: Journal of Applied Physiology. 1999 ; Vol. 87, No. 5. pp. 1678-1683.
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