Dynein light chain-dependent dimerization of Egalitarian is essential for maintaining oocyte fate in Drosophila

Hannah Neiswender, Chandler H. Goldman, Rajalakshmi Veeranan-Karmegam, Graydon B. Gonsalvez

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Egalitarian (Egl) is an RNA adaptor for the Dynein motor and is thought to link numerous, perhaps hundreds, of mRNAs with Dynein. Dynein, in turn, is responsible for the transport and localization of these mRNAs. Studies have shown that efficient mRNA binding by Egl requires the protein to dimerize. We recently demonstrated that Dynein light chain (Dlc) is responsible for facilitating the dimerization of Egl. Mutations in Egl that fail to interact with Dlc do not dimerize, and as such, are defective for mRNA binding. Consequently, this mutant does not efficiently associate with BicaudalD (BicD), the factor responsible for linking the Egl/mRNA complex with Dynein. In this report, we tested whether artificially dimerizing this Dlc-binding mutant using a leucine zipper would restore mRNA binding and rescue mutant phenotypes in vivo. Interestingly, we found that although artificial dimerization of Egl restored BicD binding, it only partially restored mRNA binding. As a result, Egl-dependent phenotypes, such as oocyte specification and mRNA localization, were only partially rescued. We hypothesize that Dlc-mediated dimerization of Egl results in a three-dimensional conformation of the Egl dimer that is best suited for mRNA binding. Although the leucine zipper restores Egl dimerization, it likely does not enable Egl to assemble into the conformation required for maximal mRNA binding activity.

Original languageEnglish (US)
Pages (from-to)76-88
Number of pages13
JournalDevelopmental Biology
Volume478
DOIs
StatePublished - Oct 2021
Externally publishedYes

Keywords

  • Cargo adaptor
  • Cell polarity
  • Molecular motor
  • RNA binding Domain
  • RNA localization

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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