E2F1 suppresses cardiac neovascularization by down-regulating VEGF and PlGF expression

Min Wu, Junlan Zhou, Min Cheng, Chan Boriboun, Dauren Biyashev, Hong Wang, Alexander Mackie, Tina Thorne, Jonathan Chou, Yiping Wu, Zhishui Chen, Qinghua Liu, Hongbin Yan, Ya Yang, Chunfa Jie, Yao Liang Tang, Ting C. Zhao, Robert N. Taylor, Raj Kishore, Douglas W. LosordoGangjian Qin

Research output: Contribution to journalArticle

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Abstract

Aims The E2F transcription factors are best characterized for their roles in cell-cycle regulation, cell growth, and cell death. Here we investigated the potential role of E2F1 in cardiac neovascularization. Methods and results We induced myocardial infarction (MI) by ligating the left anterior descending artery in wild-type (WT) and E2F1-/- mice. E2F1-/- mice demonstrated a significantly better cardiac function and smaller infarct sizes than WT mice. At infarct border zone, capillary density and endothelial cell (EC) proliferation were greater, apoptotic ECs were fewer, levels of VEGF and placental growth factor (PlGF) were higher, and p53 level was lower in E2F1-/- than in WT mice. Blockade of VEGF receptor 2 (VEGFR2) signalling with the selective inhibitor SU5416 or with the VEGFR2-blocking antibody DC101 abolished the differences between E2F1-/- mice and WT mice in cardiac function, infarct size, capillary density, EC proliferation, and EC apoptosis. In vitro, hypoxia-induced VEGF and PlGF up-regulation was significantly greater in E2F1-/- than in WT cardiac fibroblasts, and E2F1 overexpression suppressed PlGF up-regulation in both WTand p53-/- cells; however, VEGFup-regulationwas suppressed only inWTcells. E2F1 interacted with and stabilized p53 under hypoxic conditions, and both E2F1: p53 binding and the E2F1-induced suppression of VEGF promoter activity were absent in cells that expressed an N-terminally truncated E2F1 mutant. Conclusion E2F1 limits cardiac neovascularization and functional recovery after MI by suppressing VEGF and PlGF up-regulation through p53-dependent and-independent mechanisms, respectively.

Original languageEnglish (US)
Pages (from-to)412-422
Number of pages11
JournalCardiovascular Research
Volume104
Issue number3
DOIs
StatePublished - Dec 1 2014

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Vascular Endothelial Growth Factor A
Intercellular Signaling Peptides and Proteins
Vascular Endothelial Growth Factor Receptor
Up-Regulation
Endothelial Cells
Myocardial Infarction
Cell Proliferation
E2F Transcription Factors
Blocking Antibodies
Cell Cycle
Cell Death
Arteries
Fibroblasts
Apoptosis
Growth

Keywords

  • E2F1
  • Heart
  • PlGF
  • VEGF
  • p53

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Wu, M., Zhou, J., Cheng, M., Boriboun, C., Biyashev, D., Wang, H., ... Qin, G. (2014). E2F1 suppresses cardiac neovascularization by down-regulating VEGF and PlGF expression. Cardiovascular Research, 104(3), 412-422. https://doi.org/10.1093/cvr/cvu222

E2F1 suppresses cardiac neovascularization by down-regulating VEGF and PlGF expression. / Wu, Min; Zhou, Junlan; Cheng, Min; Boriboun, Chan; Biyashev, Dauren; Wang, Hong; Mackie, Alexander; Thorne, Tina; Chou, Jonathan; Wu, Yiping; Chen, Zhishui; Liu, Qinghua; Yan, Hongbin; Yang, Ya; Jie, Chunfa; Tang, Yao Liang; Zhao, Ting C.; Taylor, Robert N.; Kishore, Raj; Losordo, Douglas W.; Qin, Gangjian.

In: Cardiovascular Research, Vol. 104, No. 3, 01.12.2014, p. 412-422.

Research output: Contribution to journalArticle

Wu, M, Zhou, J, Cheng, M, Boriboun, C, Biyashev, D, Wang, H, Mackie, A, Thorne, T, Chou, J, Wu, Y, Chen, Z, Liu, Q, Yan, H, Yang, Y, Jie, C, Tang, YL, Zhao, TC, Taylor, RN, Kishore, R, Losordo, DW & Qin, G 2014, 'E2F1 suppresses cardiac neovascularization by down-regulating VEGF and PlGF expression', Cardiovascular Research, vol. 104, no. 3, pp. 412-422. https://doi.org/10.1093/cvr/cvu222
Wu, Min ; Zhou, Junlan ; Cheng, Min ; Boriboun, Chan ; Biyashev, Dauren ; Wang, Hong ; Mackie, Alexander ; Thorne, Tina ; Chou, Jonathan ; Wu, Yiping ; Chen, Zhishui ; Liu, Qinghua ; Yan, Hongbin ; Yang, Ya ; Jie, Chunfa ; Tang, Yao Liang ; Zhao, Ting C. ; Taylor, Robert N. ; Kishore, Raj ; Losordo, Douglas W. ; Qin, Gangjian. / E2F1 suppresses cardiac neovascularization by down-regulating VEGF and PlGF expression. In: Cardiovascular Research. 2014 ; Vol. 104, No. 3. pp. 412-422.
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abstract = "Aims The E2F transcription factors are best characterized for their roles in cell-cycle regulation, cell growth, and cell death. Here we investigated the potential role of E2F1 in cardiac neovascularization. Methods and results We induced myocardial infarction (MI) by ligating the left anterior descending artery in wild-type (WT) and E2F1-/- mice. E2F1-/- mice demonstrated a significantly better cardiac function and smaller infarct sizes than WT mice. At infarct border zone, capillary density and endothelial cell (EC) proliferation were greater, apoptotic ECs were fewer, levels of VEGF and placental growth factor (PlGF) were higher, and p53 level was lower in E2F1-/- than in WT mice. Blockade of VEGF receptor 2 (VEGFR2) signalling with the selective inhibitor SU5416 or with the VEGFR2-blocking antibody DC101 abolished the differences between E2F1-/- mice and WT mice in cardiac function, infarct size, capillary density, EC proliferation, and EC apoptosis. In vitro, hypoxia-induced VEGF and PlGF up-regulation was significantly greater in E2F1-/- than in WT cardiac fibroblasts, and E2F1 overexpression suppressed PlGF up-regulation in both WTand p53-/- cells; however, VEGFup-regulationwas suppressed only inWTcells. E2F1 interacted with and stabilized p53 under hypoxic conditions, and both E2F1: p53 binding and the E2F1-induced suppression of VEGF promoter activity were absent in cells that expressed an N-terminally truncated E2F1 mutant. Conclusion E2F1 limits cardiac neovascularization and functional recovery after MI by suppressing VEGF and PlGF up-regulation through p53-dependent and-independent mechanisms, respectively.",
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T1 - E2F1 suppresses cardiac neovascularization by down-regulating VEGF and PlGF expression

AU - Wu, Min

AU - Zhou, Junlan

AU - Cheng, Min

AU - Boriboun, Chan

AU - Biyashev, Dauren

AU - Wang, Hong

AU - Mackie, Alexander

AU - Thorne, Tina

AU - Chou, Jonathan

AU - Wu, Yiping

AU - Chen, Zhishui

AU - Liu, Qinghua

AU - Yan, Hongbin

AU - Yang, Ya

AU - Jie, Chunfa

AU - Tang, Yao Liang

AU - Zhao, Ting C.

AU - Taylor, Robert N.

AU - Kishore, Raj

AU - Losordo, Douglas W.

AU - Qin, Gangjian

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Aims The E2F transcription factors are best characterized for their roles in cell-cycle regulation, cell growth, and cell death. Here we investigated the potential role of E2F1 in cardiac neovascularization. Methods and results We induced myocardial infarction (MI) by ligating the left anterior descending artery in wild-type (WT) and E2F1-/- mice. E2F1-/- mice demonstrated a significantly better cardiac function and smaller infarct sizes than WT mice. At infarct border zone, capillary density and endothelial cell (EC) proliferation were greater, apoptotic ECs were fewer, levels of VEGF and placental growth factor (PlGF) were higher, and p53 level was lower in E2F1-/- than in WT mice. Blockade of VEGF receptor 2 (VEGFR2) signalling with the selective inhibitor SU5416 or with the VEGFR2-blocking antibody DC101 abolished the differences between E2F1-/- mice and WT mice in cardiac function, infarct size, capillary density, EC proliferation, and EC apoptosis. In vitro, hypoxia-induced VEGF and PlGF up-regulation was significantly greater in E2F1-/- than in WT cardiac fibroblasts, and E2F1 overexpression suppressed PlGF up-regulation in both WTand p53-/- cells; however, VEGFup-regulationwas suppressed only inWTcells. E2F1 interacted with and stabilized p53 under hypoxic conditions, and both E2F1: p53 binding and the E2F1-induced suppression of VEGF promoter activity were absent in cells that expressed an N-terminally truncated E2F1 mutant. Conclusion E2F1 limits cardiac neovascularization and functional recovery after MI by suppressing VEGF and PlGF up-regulation through p53-dependent and-independent mechanisms, respectively.

AB - Aims The E2F transcription factors are best characterized for their roles in cell-cycle regulation, cell growth, and cell death. Here we investigated the potential role of E2F1 in cardiac neovascularization. Methods and results We induced myocardial infarction (MI) by ligating the left anterior descending artery in wild-type (WT) and E2F1-/- mice. E2F1-/- mice demonstrated a significantly better cardiac function and smaller infarct sizes than WT mice. At infarct border zone, capillary density and endothelial cell (EC) proliferation were greater, apoptotic ECs were fewer, levels of VEGF and placental growth factor (PlGF) were higher, and p53 level was lower in E2F1-/- than in WT mice. Blockade of VEGF receptor 2 (VEGFR2) signalling with the selective inhibitor SU5416 or with the VEGFR2-blocking antibody DC101 abolished the differences between E2F1-/- mice and WT mice in cardiac function, infarct size, capillary density, EC proliferation, and EC apoptosis. In vitro, hypoxia-induced VEGF and PlGF up-regulation was significantly greater in E2F1-/- than in WT cardiac fibroblasts, and E2F1 overexpression suppressed PlGF up-regulation in both WTand p53-/- cells; however, VEGFup-regulationwas suppressed only inWTcells. E2F1 interacted with and stabilized p53 under hypoxic conditions, and both E2F1: p53 binding and the E2F1-induced suppression of VEGF promoter activity were absent in cells that expressed an N-terminally truncated E2F1 mutant. Conclusion E2F1 limits cardiac neovascularization and functional recovery after MI by suppressing VEGF and PlGF up-regulation through p53-dependent and-independent mechanisms, respectively.

KW - E2F1

KW - Heart

KW - PlGF

KW - VEGF

KW - p53

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