EagI and NotI linking clones from human chromosomes 11 and Xp

Mark A. Pook, Rekhaben Thakrar, Bruce Pottinger, Brian Harding, David Porteous, Veronica Heningen, John Kenneth Cowell, Carol Jones, Sue Povey, Kay E. Davies, Rajesh V. Thakker

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

EagI and NotI linking libraries were prepared in the lambda vector, EMBL5, from the mouse-human somatic cell hybrid 1W1LA4.9, which contains human chromosomes 11 and Xp as the only human component. Individual clones containing human DNA were isolated by their ability to hybridise with total human DNA and digested with SalI and EcoRI to identify the human insert size and single-copy fragments. The mean (± SD) insert sizes of the EagI and NotI clones were 18.3 ± 3.2 kb and 16.6 ± 3.6 kb, respectively. Regional localisation of 66 clones (52 EagI, 14 NotI) was achieved using a panel of 20 somatic cell hybrids that contained different overlapping deletions of chromosomes 11 or Xp. Thirty-nine clones (36 EagI, 3 NotI) were localised to chromosome 11; 17 of these were clustered in 11q13 and another nine were clustered in 11q14-q23.1. Twenty-seven clones (16 EagI, 11 NotI) were localised to Xp and 10 of these were clustered in Xp11. The 66 clones were assessed for seven different microsatellite repetitive sequences; restriction fragment length polymorphisms for five clones from 11q13 were also identified. These EagI and NotI clones, which supplement those previously mapped to chromosome 11 and Xp, should facilitate the generation of more detailed maps and the identification of genes that are associated with CpG-rich islands.

Original languageEnglish (US)
Pages (from-to)742-749
Number of pages8
JournalHuman Genetics
Volume97
Issue number6
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 11
Human Chromosomes
Clone Cells
Hybrid Cells
Chromosomes, Human, Pair 17
CpG Islands
Nucleic Acid Repetitive Sequences
DNA
Restriction Fragment Length Polymorphisms
Microsatellite Repeats
Libraries

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Pook, M. A., Thakrar, R., Pottinger, B., Harding, B., Porteous, D., Heningen, V., ... Thakker, R. V. (1996). EagI and NotI linking clones from human chromosomes 11 and Xp. Human Genetics, 97(6), 742-749. https://doi.org/10.1007/BF02346183

EagI and NotI linking clones from human chromosomes 11 and Xp. / Pook, Mark A.; Thakrar, Rekhaben; Pottinger, Bruce; Harding, Brian; Porteous, David; Heningen, Veronica; Cowell, John Kenneth; Jones, Carol; Povey, Sue; Davies, Kay E.; Thakker, Rajesh V.

In: Human Genetics, Vol. 97, No. 6, 01.01.1996, p. 742-749.

Research output: Contribution to journalArticle

Pook, MA, Thakrar, R, Pottinger, B, Harding, B, Porteous, D, Heningen, V, Cowell, JK, Jones, C, Povey, S, Davies, KE & Thakker, RV 1996, 'EagI and NotI linking clones from human chromosomes 11 and Xp', Human Genetics, vol. 97, no. 6, pp. 742-749. https://doi.org/10.1007/BF02346183
Pook MA, Thakrar R, Pottinger B, Harding B, Porteous D, Heningen V et al. EagI and NotI linking clones from human chromosomes 11 and Xp. Human Genetics. 1996 Jan 1;97(6):742-749. https://doi.org/10.1007/BF02346183
Pook, Mark A. ; Thakrar, Rekhaben ; Pottinger, Bruce ; Harding, Brian ; Porteous, David ; Heningen, Veronica ; Cowell, John Kenneth ; Jones, Carol ; Povey, Sue ; Davies, Kay E. ; Thakker, Rajesh V. / EagI and NotI linking clones from human chromosomes 11 and Xp. In: Human Genetics. 1996 ; Vol. 97, No. 6. pp. 742-749.
@article{09b213b4d0af47f7a4ecbdaf6eec0914,
title = "EagI and NotI linking clones from human chromosomes 11 and Xp",
abstract = "EagI and NotI linking libraries were prepared in the lambda vector, EMBL5, from the mouse-human somatic cell hybrid 1W1LA4.9, which contains human chromosomes 11 and Xp as the only human component. Individual clones containing human DNA were isolated by their ability to hybridise with total human DNA and digested with SalI and EcoRI to identify the human insert size and single-copy fragments. The mean (± SD) insert sizes of the EagI and NotI clones were 18.3 ± 3.2 kb and 16.6 ± 3.6 kb, respectively. Regional localisation of 66 clones (52 EagI, 14 NotI) was achieved using a panel of 20 somatic cell hybrids that contained different overlapping deletions of chromosomes 11 or Xp. Thirty-nine clones (36 EagI, 3 NotI) were localised to chromosome 11; 17 of these were clustered in 11q13 and another nine were clustered in 11q14-q23.1. Twenty-seven clones (16 EagI, 11 NotI) were localised to Xp and 10 of these were clustered in Xp11. The 66 clones were assessed for seven different microsatellite repetitive sequences; restriction fragment length polymorphisms for five clones from 11q13 were also identified. These EagI and NotI clones, which supplement those previously mapped to chromosome 11 and Xp, should facilitate the generation of more detailed maps and the identification of genes that are associated with CpG-rich islands.",
author = "Pook, {Mark A.} and Rekhaben Thakrar and Bruce Pottinger and Brian Harding and David Porteous and Veronica Heningen and Cowell, {John Kenneth} and Carol Jones and Sue Povey and Davies, {Kay E.} and Thakker, {Rajesh V.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1007/BF02346183",
language = "English (US)",
volume = "97",
pages = "742--749",
journal = "Human Genetics",
issn = "0340-6717",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - EagI and NotI linking clones from human chromosomes 11 and Xp

AU - Pook, Mark A.

AU - Thakrar, Rekhaben

AU - Pottinger, Bruce

AU - Harding, Brian

AU - Porteous, David

AU - Heningen, Veronica

AU - Cowell, John Kenneth

AU - Jones, Carol

AU - Povey, Sue

AU - Davies, Kay E.

AU - Thakker, Rajesh V.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - EagI and NotI linking libraries were prepared in the lambda vector, EMBL5, from the mouse-human somatic cell hybrid 1W1LA4.9, which contains human chromosomes 11 and Xp as the only human component. Individual clones containing human DNA were isolated by their ability to hybridise with total human DNA and digested with SalI and EcoRI to identify the human insert size and single-copy fragments. The mean (± SD) insert sizes of the EagI and NotI clones were 18.3 ± 3.2 kb and 16.6 ± 3.6 kb, respectively. Regional localisation of 66 clones (52 EagI, 14 NotI) was achieved using a panel of 20 somatic cell hybrids that contained different overlapping deletions of chromosomes 11 or Xp. Thirty-nine clones (36 EagI, 3 NotI) were localised to chromosome 11; 17 of these were clustered in 11q13 and another nine were clustered in 11q14-q23.1. Twenty-seven clones (16 EagI, 11 NotI) were localised to Xp and 10 of these were clustered in Xp11. The 66 clones were assessed for seven different microsatellite repetitive sequences; restriction fragment length polymorphisms for five clones from 11q13 were also identified. These EagI and NotI clones, which supplement those previously mapped to chromosome 11 and Xp, should facilitate the generation of more detailed maps and the identification of genes that are associated with CpG-rich islands.

AB - EagI and NotI linking libraries were prepared in the lambda vector, EMBL5, from the mouse-human somatic cell hybrid 1W1LA4.9, which contains human chromosomes 11 and Xp as the only human component. Individual clones containing human DNA were isolated by their ability to hybridise with total human DNA and digested with SalI and EcoRI to identify the human insert size and single-copy fragments. The mean (± SD) insert sizes of the EagI and NotI clones were 18.3 ± 3.2 kb and 16.6 ± 3.6 kb, respectively. Regional localisation of 66 clones (52 EagI, 14 NotI) was achieved using a panel of 20 somatic cell hybrids that contained different overlapping deletions of chromosomes 11 or Xp. Thirty-nine clones (36 EagI, 3 NotI) were localised to chromosome 11; 17 of these were clustered in 11q13 and another nine were clustered in 11q14-q23.1. Twenty-seven clones (16 EagI, 11 NotI) were localised to Xp and 10 of these were clustered in Xp11. The 66 clones were assessed for seven different microsatellite repetitive sequences; restriction fragment length polymorphisms for five clones from 11q13 were also identified. These EagI and NotI clones, which supplement those previously mapped to chromosome 11 and Xp, should facilitate the generation of more detailed maps and the identification of genes that are associated with CpG-rich islands.

UR - http://www.scopus.com/inward/record.url?scp=15844362454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=15844362454&partnerID=8YFLogxK

U2 - 10.1007/BF02346183

DO - 10.1007/BF02346183

M3 - Article

VL - 97

SP - 742

EP - 749

JO - Human Genetics

JF - Human Genetics

SN - 0340-6717

IS - 6

ER -