Early and late stimulation of ob mRNA expression in meal-fed and overfed rats

R. B.S. Harris, T. G. Ramsay, S. R. Smith, R. C. Bruch

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

ob protein is hypothesized to be a circulating feedback signal in the regulation of energy balance. Obese, overfed rats have high levels of ob mRNA expression and suppressed voluntary food intake, indicating the presence of a potent satiety factor. The objectives of this experiment were to determine whether feeding rats their normal daily intake in three meals, compared with ad libitum feeding, increased ob mRNA expression and to determine the degree of obesity required to stimulate expression of ob mRNA. Rats were fed ad libitum, were tube-fed their normal intake in three meals a day, or were tube-fed twice normal intake. ob mRNA was measured by Northern blot analysis after 0, 2, 7, 14, 21, and 32 d of tube-feeding. After only 2 d ob mRNA was threefold higher in tube-fed animals than in ad libitum controls. By day 21 there was a further increase in ob mRNA expression in overfed rats which were at 130% control weight. These results suggest that a metabolic consequence of meal-feeding increases ob mRNA expression in the absence of increased food intake or weight gain. There is a further increase in ob mRNA expression once significant obesity is established.

Original languageEnglish (US)
Pages (from-to)2020-2026
Number of pages7
JournalJournal of Clinical Investigation
Volume97
Issue number9
DOIs
StatePublished - Jan 1 1996

Fingerprint

Meals
Messenger RNA
Obesity
Eating
Enteral Nutrition
Leptin
Northern Blotting
Weight Gain
Weights and Measures

Keywords

  • Northern blot
  • energy balance
  • ob protein
  • obesity
  • rats

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Early and late stimulation of ob mRNA expression in meal-fed and overfed rats. / Harris, R. B.S.; Ramsay, T. G.; Smith, S. R.; Bruch, R. C.

In: Journal of Clinical Investigation, Vol. 97, No. 9, 01.01.1996, p. 2020-2026.

Research output: Contribution to journalArticle

Harris, R. B.S. ; Ramsay, T. G. ; Smith, S. R. ; Bruch, R. C. / Early and late stimulation of ob mRNA expression in meal-fed and overfed rats. In: Journal of Clinical Investigation. 1996 ; Vol. 97, No. 9. pp. 2020-2026.
@article{20dd497ab11e40219a3ee0ae6cce5206,
title = "Early and late stimulation of ob mRNA expression in meal-fed and overfed rats",
abstract = "ob protein is hypothesized to be a circulating feedback signal in the regulation of energy balance. Obese, overfed rats have high levels of ob mRNA expression and suppressed voluntary food intake, indicating the presence of a potent satiety factor. The objectives of this experiment were to determine whether feeding rats their normal daily intake in three meals, compared with ad libitum feeding, increased ob mRNA expression and to determine the degree of obesity required to stimulate expression of ob mRNA. Rats were fed ad libitum, were tube-fed their normal intake in three meals a day, or were tube-fed twice normal intake. ob mRNA was measured by Northern blot analysis after 0, 2, 7, 14, 21, and 32 d of tube-feeding. After only 2 d ob mRNA was threefold higher in tube-fed animals than in ad libitum controls. By day 21 there was a further increase in ob mRNA expression in overfed rats which were at 130{\%} control weight. These results suggest that a metabolic consequence of meal-feeding increases ob mRNA expression in the absence of increased food intake or weight gain. There is a further increase in ob mRNA expression once significant obesity is established.",
keywords = "Northern blot, energy balance, ob protein, obesity, rats",
author = "Harris, {R. B.S.} and Ramsay, {T. G.} and Smith, {S. R.} and Bruch, {R. C.}",
year = "1996",
month = "1",
day = "1",
doi = "10.1172/JCI118637",
language = "English (US)",
volume = "97",
pages = "2020--2026",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "9",

}

TY - JOUR

T1 - Early and late stimulation of ob mRNA expression in meal-fed and overfed rats

AU - Harris, R. B.S.

AU - Ramsay, T. G.

AU - Smith, S. R.

AU - Bruch, R. C.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - ob protein is hypothesized to be a circulating feedback signal in the regulation of energy balance. Obese, overfed rats have high levels of ob mRNA expression and suppressed voluntary food intake, indicating the presence of a potent satiety factor. The objectives of this experiment were to determine whether feeding rats their normal daily intake in three meals, compared with ad libitum feeding, increased ob mRNA expression and to determine the degree of obesity required to stimulate expression of ob mRNA. Rats were fed ad libitum, were tube-fed their normal intake in three meals a day, or were tube-fed twice normal intake. ob mRNA was measured by Northern blot analysis after 0, 2, 7, 14, 21, and 32 d of tube-feeding. After only 2 d ob mRNA was threefold higher in tube-fed animals than in ad libitum controls. By day 21 there was a further increase in ob mRNA expression in overfed rats which were at 130% control weight. These results suggest that a metabolic consequence of meal-feeding increases ob mRNA expression in the absence of increased food intake or weight gain. There is a further increase in ob mRNA expression once significant obesity is established.

AB - ob protein is hypothesized to be a circulating feedback signal in the regulation of energy balance. Obese, overfed rats have high levels of ob mRNA expression and suppressed voluntary food intake, indicating the presence of a potent satiety factor. The objectives of this experiment were to determine whether feeding rats their normal daily intake in three meals, compared with ad libitum feeding, increased ob mRNA expression and to determine the degree of obesity required to stimulate expression of ob mRNA. Rats were fed ad libitum, were tube-fed their normal intake in three meals a day, or were tube-fed twice normal intake. ob mRNA was measured by Northern blot analysis after 0, 2, 7, 14, 21, and 32 d of tube-feeding. After only 2 d ob mRNA was threefold higher in tube-fed animals than in ad libitum controls. By day 21 there was a further increase in ob mRNA expression in overfed rats which were at 130% control weight. These results suggest that a metabolic consequence of meal-feeding increases ob mRNA expression in the absence of increased food intake or weight gain. There is a further increase in ob mRNA expression once significant obesity is established.

KW - Northern blot

KW - energy balance

KW - ob protein

KW - obesity

KW - rats

UR - http://www.scopus.com/inward/record.url?scp=0029976373&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029976373&partnerID=8YFLogxK

U2 - 10.1172/JCI118637

DO - 10.1172/JCI118637

M3 - Article

C2 - 8621790

AN - SCOPUS:0029976373

VL - 97

SP - 2020

EP - 2026

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 9

ER -