Effect of alanine concentration independent of changes in insulin and glucagon on alanine and glucose homeostasis in the conscious dog

Michael Peter Diamond, Robert C. Rollings, Kurt E. Steiner, Phillip E. Williams, William W. Lacy, Alan D. Cherrington

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effect of an alanine load per se on hepatic alanine balance and hepatic glucose production is unclear. To examine this question, alanine was infused into six postabsorptive dogs at a rate of 6 μmol/kg-min, while maintaining insulin and glucagon levels using the pancreatic clamp technique. The arterial alanine concentration rose from a basal level of 227 ± 16 μmol/L to 497 ± 40 μmol/L during alanine infusion (P < .01). The net hepatic fractional extraction of alanine remained unchanged, while hepatic alanine uptake increased from 3.0 ± 0.3 to 6.0 ± 0.4 μmol/kg-min (P < .01). Conversion of alanine into glucose increased 87% to 2.7 ± 0.3 μmol/kg-min during alanine infusion (P < .01) while gluconeogenic efficiency remained essentially unchanged. Despite the increased gluconeogenic rate, the total rate of glucose production was unchanged. These data suggest that an increase in the alanine load to the liver causes a proportional increase in net hepatic alanine uptake and the gluconeogenic rate, but that in an overnight fasted animal this increase is insufficient to significantly increase glucose production.

Original languageEnglish (US)
Pages (from-to)28-33
Number of pages6
JournalMetabolism
Volume37
Issue number1
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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Glucagon
Alanine
Homeostasis
Dogs
Insulin
Glucose
Liver

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Effect of alanine concentration independent of changes in insulin and glucagon on alanine and glucose homeostasis in the conscious dog. / Diamond, Michael Peter; Rollings, Robert C.; Steiner, Kurt E.; Williams, Phillip E.; Lacy, William W.; Cherrington, Alan D.

In: Metabolism, Vol. 37, No. 1, 01.01.1988, p. 28-33.

Research output: Contribution to journalArticle

Diamond, Michael Peter ; Rollings, Robert C. ; Steiner, Kurt E. ; Williams, Phillip E. ; Lacy, William W. ; Cherrington, Alan D. / Effect of alanine concentration independent of changes in insulin and glucagon on alanine and glucose homeostasis in the conscious dog. In: Metabolism. 1988 ; Vol. 37, No. 1. pp. 28-33.
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