Effect of bacterial collagenase on resin-dentin bonds degradation

Manuel Toledano, Raquel Osorio, Estrella Osorio, Fátima S. Aguilera, Monica Yamauti, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The objective of this study is to evaluate the effect of a bacterial collagenase on the degradation of resin-dentin bonds. Human dentin surfaces were bonded with: an etch-&-rinse self-priming adhesive (SB), a two-step self-etching primer/adhesive (SEB), and a 1-step self-etching adhesive (OUB). Composite build-ups were constructed. The bonded teeth were stored (24 h, 3 months, 1 year) in distilled water or in a buffered bacterial collagenase solution. Half of the specimens were stored as intact bonded teeth (Indirect Exposure/IE). The other half were sectioned into beams prior to storage (Direct Exposure/DE). After storage the intact teeth were sectioned into beams and all specimens were tested for microtensile bond strengths (MTBS). ANOVA and multiple comparisons tests were performed. Fractographic analysis was performed by scanning electron microscopy. The inclusion of bacterial collagenase in the storing solution did not lower the MTBS values over those seen in specimens stored in water. SB and SEB bonds strength were equal, and were superior to OUB. After 3 months of DE, SB and OUB bonded specimens showed decreases in MTBS; similar reductions required 1 year for SEB/DE. MTBS did not decrease in IE specimens except for OUB. Resin and collagen dissolution were evident in DE groups after storing.

Original languageEnglish (US)
Pages (from-to)2355-2361
Number of pages7
JournalJournal of Materials Science: Materials in Medicine
Volume18
Issue number12
DOIs
StatePublished - Dec 1 2007

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Collagenases
Dentin
Adhesives
Tooth
Resins
Degradation
Etching
Water
Analysis of variance (ANOVA)
Collagen
Electron Scanning Microscopy
Analysis of Variance
Dissolution
Scanning electron microscopy
Composite materials

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Effect of bacterial collagenase on resin-dentin bonds degradation. / Toledano, Manuel; Osorio, Raquel; Osorio, Estrella; Aguilera, Fátima S.; Yamauti, Monica; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Materials Science: Materials in Medicine, Vol. 18, No. 12, 01.12.2007, p. 2355-2361.

Research output: Contribution to journalArticle

Toledano, M, Osorio, R, Osorio, E, Aguilera, FS, Yamauti, M, Pashley, DH & Tay, FCM 2007, 'Effect of bacterial collagenase on resin-dentin bonds degradation', Journal of Materials Science: Materials in Medicine, vol. 18, no. 12, pp. 2355-2361. https://doi.org/10.1007/s10856-007-3161-z
Toledano M, Osorio R, Osorio E, Aguilera FS, Yamauti M, Pashley DH et al. Effect of bacterial collagenase on resin-dentin bonds degradation. Journal of Materials Science: Materials in Medicine. 2007 Dec 1;18(12):2355-2361. https://doi.org/10.1007/s10856-007-3161-z
Toledano, Manuel ; Osorio, Raquel ; Osorio, Estrella ; Aguilera, Fátima S. ; Yamauti, Monica ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Effect of bacterial collagenase on resin-dentin bonds degradation. In: Journal of Materials Science: Materials in Medicine. 2007 ; Vol. 18, No. 12. pp. 2355-2361.
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