Effect of caffeine on phosphoinositide metabolism in frog skeletal muscle

Z. Dong, P. H. Zhu

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

High K+ exposure has been shown to activate phosphoinositide turnover in skeletal muscle. To clarify the mechanism of this activation, we have studied the effect of caffeine on the metabolism of inositol phospholipids using [3H]myo-inositol labeled skeletal muscle of the frog. Caffeine significantly changed the phosphoinositide metabolism in a dose- and time-dependent manner. Being different from that of high K+, the effect of caffeine was characterized by an increase of the labeled phosphatidylinositol 4,5-bisphosphate (PIP2) and a decrease of the labeled inositol bisphosphate (IP2). In addition, high K+ supplemented during caffeine treatment caused an increase of the labeled inositol phosphates and a decrease of the labeled phosphatidylinositol 4-phosphate (PIP) and PIP2. It is suggested that the myoplasmic Ca2+ increase produced by high K+ exposure is not a sole factor for the activation of phosphoinositide metabolism, and the high K+-induced depolarization also plays a role.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalChinese Journal of Physiological Sciences
Volume12
Issue number2
StatePublished - Dec 1 1996
Externally publishedYes

Keywords

  • caffeine
  • excitation-contraction coupling
  • phosphoinositide
  • skeletal muscle

ASJC Scopus subject areas

  • Physiology

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