Effect of glyburide on myocardial metabolism and function

Stephen W. Schaffer, Boen H. Tan, Mahmood S Mozaffari

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Exposure of perfused rat hearts to the second-generation sulfonylurea glyburide led to a dramatic increase in glycolytic flux and lactate production. Maximal response in the absence of insulin occurred at a concentration of 2.8 μM and resulted in a 45 percent increase in the glucose utilization rate. When insulin was included in the buffer, glyburide response was significantly increased. Similarly, glyburide potentiated the metabolic effects of insulin. Since glyburide did not promote glycogenolysis, the increase in glycolysis was caused solely by the rise in glucose utilization. The classic cross-over plot for glycolytic intermediates and transport showed that glyburide stimulates glycolysis by activating the rate-limiting enzyme phosphofructokinase and promoting glucose transport. Glycolytic intermediate data also suggested that the sulfonylurea promotes oxidation of pyruvate via the citric acid cycle. Since the drug does not alter oxygen consumption, the contribution of glucose to overall adenosine triphosphate production rises while that of fatty acids falls. These metabolic changes aid the heart in resisting an ischemic insult.

Original languageEnglish (US)
Pages (from-to)48-52
Number of pages5
JournalThe American Journal of Medicine
Volume79
Issue number3 SUPPL. 2
DOIs
StatePublished - Sep 20 1985
Externally publishedYes

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Glyburide
Glucose
Glycolysis
Insulin
Glycogenolysis
Phosphofructokinases
Citric Acid Cycle
Pyruvic Acid
Oxygen Consumption
Lactic Acid
Buffers
Fatty Acids
Adenosine Triphosphate
Enzymes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of glyburide on myocardial metabolism and function. / Schaffer, Stephen W.; Tan, Boen H.; Mozaffari, Mahmood S.

In: The American Journal of Medicine, Vol. 79, No. 3 SUPPL. 2, 20.09.1985, p. 48-52.

Research output: Contribution to journalArticle

Schaffer, Stephen W. ; Tan, Boen H. ; Mozaffari, Mahmood S. / Effect of glyburide on myocardial metabolism and function. In: The American Journal of Medicine. 1985 ; Vol. 79, No. 3 SUPPL. 2. pp. 48-52.
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