Effect of phosphoric acid on the degradation of human dentin matrix

A. Tezvergil-Mutluay, M. Mutluay, R. Seseogullari-Dirihan, K. A. Agee, W. O. Key, D. L.S. Scheffel, L. Breschi, A. Mazzoni, L. Tjäderhane, Y. Nishitani, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

This study determined if dentin proteases are denatured by phosphoric acid (PA) used in etch-and-rinse dentin adhesives. Dentin beams were completely demineralized with EDTA for 30 days. We "acid-etched" experimental groups by exposing the demineralized dentin beams to 1, 10, or 37 mass% PA for 15 sec or 15 min. Control beams were not exposed to PA but were incubated in simulated body fluid for 3 days to assay their total endogenous telopeptidase activity, by their ability to solubilize C-terminal crosslinked telopeptides ICTP and CTX from insoluble dentin collagen. Control beams released 6.1 ± 0.8 ng ICTP and 0.6 ± 0.1 ng CTX/mg dry-wt/3 days. Positive control beams pre-incubated in p-aminophenylmercuric acetate, a compound known to activate proMMPs, released about the same amount of ICTP peptides, but released significantly less CTX. Beams immersed in 1, 10, or 37 mass% PA for 15 sec or 15 min released amounts of ICTP and CTX similar to that released by the controls (p > 0.05). Beams incubated in galardin, an MMP inhibitor, or E-64, a cathepsin inhibitor, blocked most of the release of ICTP and CTX, respectively. It is concluded that PA does not denature endogenous MMP and cathepsin activities of dentin matrices.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalJournal of Dental Research
Volume92
Issue number1
DOIs
StatePublished - Jan 1 2013

Fingerprint

Dentin
Cathepsins
Matrix Metalloproteinase Inhibitors
Body Fluids
Matrix Metalloproteinases
Edetic Acid
Adhesives
phosphoric acid
Acetates
Peptide Hydrolases
Collagen
Acids

Keywords

  • MMPs
  • bonding
  • cathepsins
  • collagen
  • demineralized

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Tezvergil-Mutluay, A., Mutluay, M., Seseogullari-Dirihan, R., Agee, K. A., Key, W. O., Scheffel, D. L. S., ... Pashley, D. H. (2013). Effect of phosphoric acid on the degradation of human dentin matrix. Journal of Dental Research, 92(1), 87-91. https://doi.org/10.1177/0022034512466264

Effect of phosphoric acid on the degradation of human dentin matrix. / Tezvergil-Mutluay, A.; Mutluay, M.; Seseogullari-Dirihan, R.; Agee, K. A.; Key, W. O.; Scheffel, D. L.S.; Breschi, L.; Mazzoni, A.; Tjäderhane, L.; Nishitani, Y.; Tay, Franklin Chi Meng; Pashley, David Henry.

In: Journal of Dental Research, Vol. 92, No. 1, 01.01.2013, p. 87-91.

Research output: Contribution to journalArticle

Tezvergil-Mutluay, A, Mutluay, M, Seseogullari-Dirihan, R, Agee, KA, Key, WO, Scheffel, DLS, Breschi, L, Mazzoni, A, Tjäderhane, L, Nishitani, Y, Tay, FCM & Pashley, DH 2013, 'Effect of phosphoric acid on the degradation of human dentin matrix', Journal of Dental Research, vol. 92, no. 1, pp. 87-91. https://doi.org/10.1177/0022034512466264
Tezvergil-Mutluay A, Mutluay M, Seseogullari-Dirihan R, Agee KA, Key WO, Scheffel DLS et al. Effect of phosphoric acid on the degradation of human dentin matrix. Journal of Dental Research. 2013 Jan 1;92(1):87-91. https://doi.org/10.1177/0022034512466264
Tezvergil-Mutluay, A. ; Mutluay, M. ; Seseogullari-Dirihan, R. ; Agee, K. A. ; Key, W. O. ; Scheffel, D. L.S. ; Breschi, L. ; Mazzoni, A. ; Tjäderhane, L. ; Nishitani, Y. ; Tay, Franklin Chi Meng ; Pashley, David Henry. / Effect of phosphoric acid on the degradation of human dentin matrix. In: Journal of Dental Research. 2013 ; Vol. 92, No. 1. pp. 87-91.
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