Effect of polyacrylic acid on dentin protease activities

S. Ozcan, R. Seseogullari-Dirihan, M. Uctasli, F. R. Tay, D. H. Pashley, A. Tezvergil-Mutluay

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective This study tested whether treatment of demineralized dentin with polyacrylic acid (PAA) has any activatory or inhibitory activity on dentin matrix metalloproteinases (MMP)s or cathepsin K (CAT-K). Methods Dentin beams (1 mm × 2 mm × 6 mm; n = 10) were completely demineralized with EDTA. After initial dry mass assessment, the beams were dipped into 37% phosphoric acid (PA), PA + 2% benzalkonium chloride (BAC), PA + 2% chlorhexidine digluconate (CHX), 10% PAA, PAA + BAC or PAA + CHX for 20 s. Demineralized beams without treatment served as control. All beams were incubated in simulated body fluid (SBF) for 1 week and the dry mass loss was evaluated. Aliquots of SBF were used to analyze solubilized telopeptide fragments using ICTP as indicator of MMP-mediated collagen degradation and CTX for CAT-K-mediated degradation. Additional demineralized beams (n = 10) were used to measure the influence of different chemical treatments on total MMP activity of EDTA-demineralized dentin using generic MMP assay. Data were analyzed by ANOVA (α = 0.05). Results Dry mass loss ranged from 6% (PA) to 2% for (PA-BAC) or (PAA-BAC) (p < 0.05). ICTP release of PAA-treated group was significantly higher (p < 0.05) than the control, and not significantly different from the PA group (p > 0.05). PA + CHX or PAA + CHX and PAA + BAC showed significantly lower ICTP than PA or PAA groups (p < 0.05). CAT-K activity increased significantly after 10% PAA treatment compared to control (p < 0.05) or to PA postreatment. Significance Demineralized dentin treated with 10% polyacrylic acid activated CAT-K more than 37% phosphoric acid; 2% chlorhexidine digluconate seems to be a better inhibitor of MMPs and CAT-K than 2% benzalkonium chloride.

Original languageEnglish (US)
Pages (from-to)901-906
Number of pages6
JournalDental Materials
Volume31
Issue number8
DOIs
StatePublished - Aug 1 2015

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carbopol 940
Dentin
Phosphoric acid
Benzalkonium Compounds
Peptide Hydrolases
Cathepsin K
Acids
Matrix Metalloproteinases
Body fluids
Ethylenediaminetetraacetic acid
Body Fluids
Edetic Acid
phosphoric acid
Degradation
Matrix Metalloproteinase Inhibitors

Keywords

  • Cysteine cathepsins
  • Degradation
  • Dentin
  • Matrix metalloproteinase
  • Polyacrylic acid

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Ozcan, S., Seseogullari-Dirihan, R., Uctasli, M., Tay, F. R., Pashley, D. H., & Tezvergil-Mutluay, A. (2015). Effect of polyacrylic acid on dentin protease activities. Dental Materials, 31(8), 901-906. https://doi.org/10.1016/j.dental.2015.04.018

Effect of polyacrylic acid on dentin protease activities. / Ozcan, S.; Seseogullari-Dirihan, R.; Uctasli, M.; Tay, F. R.; Pashley, D. H.; Tezvergil-Mutluay, A.

In: Dental Materials, Vol. 31, No. 8, 01.08.2015, p. 901-906.

Research output: Contribution to journalArticle

Ozcan, S, Seseogullari-Dirihan, R, Uctasli, M, Tay, FR, Pashley, DH & Tezvergil-Mutluay, A 2015, 'Effect of polyacrylic acid on dentin protease activities', Dental Materials, vol. 31, no. 8, pp. 901-906. https://doi.org/10.1016/j.dental.2015.04.018
Ozcan S, Seseogullari-Dirihan R, Uctasli M, Tay FR, Pashley DH, Tezvergil-Mutluay A. Effect of polyacrylic acid on dentin protease activities. Dental Materials. 2015 Aug 1;31(8):901-906. https://doi.org/10.1016/j.dental.2015.04.018
Ozcan, S. ; Seseogullari-Dirihan, R. ; Uctasli, M. ; Tay, F. R. ; Pashley, D. H. ; Tezvergil-Mutluay, A. / Effect of polyacrylic acid on dentin protease activities. In: Dental Materials. 2015 ; Vol. 31, No. 8. pp. 901-906.
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abstract = "Objective This study tested whether treatment of demineralized dentin with polyacrylic acid (PAA) has any activatory or inhibitory activity on dentin matrix metalloproteinases (MMP)s or cathepsin K (CAT-K). Methods Dentin beams (1 mm × 2 mm × 6 mm; n = 10) were completely demineralized with EDTA. After initial dry mass assessment, the beams were dipped into 37{\%} phosphoric acid (PA), PA + 2{\%} benzalkonium chloride (BAC), PA + 2{\%} chlorhexidine digluconate (CHX), 10{\%} PAA, PAA + BAC or PAA + CHX for 20 s. Demineralized beams without treatment served as control. All beams were incubated in simulated body fluid (SBF) for 1 week and the dry mass loss was evaluated. Aliquots of SBF were used to analyze solubilized telopeptide fragments using ICTP as indicator of MMP-mediated collagen degradation and CTX for CAT-K-mediated degradation. Additional demineralized beams (n = 10) were used to measure the influence of different chemical treatments on total MMP activity of EDTA-demineralized dentin using generic MMP assay. Data were analyzed by ANOVA (α = 0.05). Results Dry mass loss ranged from 6{\%} (PA) to 2{\%} for (PA-BAC) or (PAA-BAC) (p < 0.05). ICTP release of PAA-treated group was significantly higher (p < 0.05) than the control, and not significantly different from the PA group (p > 0.05). PA + CHX or PAA + CHX and PAA + BAC showed significantly lower ICTP than PA or PAA groups (p < 0.05). CAT-K activity increased significantly after 10{\%} PAA treatment compared to control (p < 0.05) or to PA postreatment. Significance Demineralized dentin treated with 10{\%} polyacrylic acid activated CAT-K more than 37{\%} phosphoric acid; 2{\%} chlorhexidine digluconate seems to be a better inhibitor of MMPs and CAT-K than 2{\%} benzalkonium chloride.",
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AU - Ozcan, S.

AU - Seseogullari-Dirihan, R.

AU - Uctasli, M.

AU - Tay, F. R.

AU - Pashley, D. H.

AU - Tezvergil-Mutluay, A.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Objective This study tested whether treatment of demineralized dentin with polyacrylic acid (PAA) has any activatory or inhibitory activity on dentin matrix metalloproteinases (MMP)s or cathepsin K (CAT-K). Methods Dentin beams (1 mm × 2 mm × 6 mm; n = 10) were completely demineralized with EDTA. After initial dry mass assessment, the beams were dipped into 37% phosphoric acid (PA), PA + 2% benzalkonium chloride (BAC), PA + 2% chlorhexidine digluconate (CHX), 10% PAA, PAA + BAC or PAA + CHX for 20 s. Demineralized beams without treatment served as control. All beams were incubated in simulated body fluid (SBF) for 1 week and the dry mass loss was evaluated. Aliquots of SBF were used to analyze solubilized telopeptide fragments using ICTP as indicator of MMP-mediated collagen degradation and CTX for CAT-K-mediated degradation. Additional demineralized beams (n = 10) were used to measure the influence of different chemical treatments on total MMP activity of EDTA-demineralized dentin using generic MMP assay. Data were analyzed by ANOVA (α = 0.05). Results Dry mass loss ranged from 6% (PA) to 2% for (PA-BAC) or (PAA-BAC) (p < 0.05). ICTP release of PAA-treated group was significantly higher (p < 0.05) than the control, and not significantly different from the PA group (p > 0.05). PA + CHX or PAA + CHX and PAA + BAC showed significantly lower ICTP than PA or PAA groups (p < 0.05). CAT-K activity increased significantly after 10% PAA treatment compared to control (p < 0.05) or to PA postreatment. Significance Demineralized dentin treated with 10% polyacrylic acid activated CAT-K more than 37% phosphoric acid; 2% chlorhexidine digluconate seems to be a better inhibitor of MMPs and CAT-K than 2% benzalkonium chloride.

AB - Objective This study tested whether treatment of demineralized dentin with polyacrylic acid (PAA) has any activatory or inhibitory activity on dentin matrix metalloproteinases (MMP)s or cathepsin K (CAT-K). Methods Dentin beams (1 mm × 2 mm × 6 mm; n = 10) were completely demineralized with EDTA. After initial dry mass assessment, the beams were dipped into 37% phosphoric acid (PA), PA + 2% benzalkonium chloride (BAC), PA + 2% chlorhexidine digluconate (CHX), 10% PAA, PAA + BAC or PAA + CHX for 20 s. Demineralized beams without treatment served as control. All beams were incubated in simulated body fluid (SBF) for 1 week and the dry mass loss was evaluated. Aliquots of SBF were used to analyze solubilized telopeptide fragments using ICTP as indicator of MMP-mediated collagen degradation and CTX for CAT-K-mediated degradation. Additional demineralized beams (n = 10) were used to measure the influence of different chemical treatments on total MMP activity of EDTA-demineralized dentin using generic MMP assay. Data were analyzed by ANOVA (α = 0.05). Results Dry mass loss ranged from 6% (PA) to 2% for (PA-BAC) or (PAA-BAC) (p < 0.05). ICTP release of PAA-treated group was significantly higher (p < 0.05) than the control, and not significantly different from the PA group (p > 0.05). PA + CHX or PAA + CHX and PAA + BAC showed significantly lower ICTP than PA or PAA groups (p < 0.05). CAT-K activity increased significantly after 10% PAA treatment compared to control (p < 0.05) or to PA postreatment. Significance Demineralized dentin treated with 10% polyacrylic acid activated CAT-K more than 37% phosphoric acid; 2% chlorhexidine digluconate seems to be a better inhibitor of MMPs and CAT-K than 2% benzalkonium chloride.

KW - Cysteine cathepsins

KW - Degradation

KW - Dentin

KW - Matrix metalloproteinase

KW - Polyacrylic acid

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