Effect of proanthocyanidin incorporation into dental adhesive resin on resin-dentine bond strength

D. J. Epasinghe, C. K.Y. Yiu, M. F. Burrow, Franklin Chi Meng Tay, N. M. King

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Objectives: This study evaluated the effect of proanthocyanidin (PA) incorporation into experimental dental adhesives on resin-dentine bond strength. Methods: Four experimental hydrophilic adhesives containing different PA concentrations were prepared by combining 50 wt% resin comonomer mixtures with 50 wt% ethanol. Proanthocyanidin was added to the ethanol-solvated resin to yield three adhesives with PA concentrations of 1.0, 2.0 and 3.0 wt%, respectively. A PA-free adhesive served as the control. Flat dentine surfaces from 40 extracted third molars were etched with 32% phosphoric acid. The specimens were randomly assigned to one of the four adhesive groups. Two layers of one of the four experimental adhesives were applied to the etched dentine and light-cured for 20 s. Composite build-ups were performed using Filtek Z250 (3M ESPE). After storage in distilled water at 37 °C for 24 h, twenty-four bonded teeth were sectioned into 0.9 mm × 0.9 mm beams and stressed to failure under tension for bond strength testing. Bond strength data were evaluated by one-way ANOVA and Tukey's test (α = 0.05). Interfacial nanoleakage was examined in the remaining teeth using a field-emission scanning electron microscope and analysed using the Chi-square test (α = 0.05). Results: No significant difference in bond strength was found amongst PA-free, 1% and 2% PA adhesives. However, incorporation of 3% PA into the adhesive significantly lowered bond strength as demonstrated by a greater number of adhesive failures and more extensive nanoleakage along the bonded interface. Conclusion: Incorporation of 2% proanthocyanidin into dental adhesives has no adverse effect on dentine bond strength. Clinical significance: The addition of proanthocyanidin to an experimental adhesive has no adverse effect on the immediate resin-dentine bond strength when the concentration of proanthocyanidin in the adhesive is less than or equal to 2%.

Original languageEnglish (US)
Pages (from-to)173-180
Number of pages8
JournalJournal of Dentistry
Volume40
Issue number3
DOIs
StatePublished - Mar 1 2012

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Synthetic Resins
Dental Cements
Dentin
Adhesives
Tooth
proanthocyanidin
Ethanol
Third Molar
Chi-Square Distribution
Analysis of Variance

Keywords

  • Bond strength
  • Collagen cross-linking
  • Dentine
  • Proanthocyanidin
  • Resin

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effect of proanthocyanidin incorporation into dental adhesive resin on resin-dentine bond strength. / Epasinghe, D. J.; Yiu, C. K.Y.; Burrow, M. F.; Tay, Franklin Chi Meng; King, N. M.

In: Journal of Dentistry, Vol. 40, No. 3, 01.03.2012, p. 173-180.

Research output: Contribution to journalArticle

Epasinghe, D. J. ; Yiu, C. K.Y. ; Burrow, M. F. ; Tay, Franklin Chi Meng ; King, N. M. / Effect of proanthocyanidin incorporation into dental adhesive resin on resin-dentine bond strength. In: Journal of Dentistry. 2012 ; Vol. 40, No. 3. pp. 173-180.
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abstract = "Objectives: This study evaluated the effect of proanthocyanidin (PA) incorporation into experimental dental adhesives on resin-dentine bond strength. Methods: Four experimental hydrophilic adhesives containing different PA concentrations were prepared by combining 50 wt{\%} resin comonomer mixtures with 50 wt{\%} ethanol. Proanthocyanidin was added to the ethanol-solvated resin to yield three adhesives with PA concentrations of 1.0, 2.0 and 3.0 wt{\%}, respectively. A PA-free adhesive served as the control. Flat dentine surfaces from 40 extracted third molars were etched with 32{\%} phosphoric acid. The specimens were randomly assigned to one of the four adhesive groups. Two layers of one of the four experimental adhesives were applied to the etched dentine and light-cured for 20 s. Composite build-ups were performed using Filtek Z250 (3M ESPE). After storage in distilled water at 37 °C for 24 h, twenty-four bonded teeth were sectioned into 0.9 mm × 0.9 mm beams and stressed to failure under tension for bond strength testing. Bond strength data were evaluated by one-way ANOVA and Tukey's test (α = 0.05). Interfacial nanoleakage was examined in the remaining teeth using a field-emission scanning electron microscope and analysed using the Chi-square test (α = 0.05). Results: No significant difference in bond strength was found amongst PA-free, 1{\%} and 2{\%} PA adhesives. However, incorporation of 3{\%} PA into the adhesive significantly lowered bond strength as demonstrated by a greater number of adhesive failures and more extensive nanoleakage along the bonded interface. Conclusion: Incorporation of 2{\%} proanthocyanidin into dental adhesives has no adverse effect on dentine bond strength. Clinical significance: The addition of proanthocyanidin to an experimental adhesive has no adverse effect on the immediate resin-dentine bond strength when the concentration of proanthocyanidin in the adhesive is less than or equal to 2{\%}.",
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AU - Tay, Franklin Chi Meng

AU - King, N. M.

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N2 - Objectives: This study evaluated the effect of proanthocyanidin (PA) incorporation into experimental dental adhesives on resin-dentine bond strength. Methods: Four experimental hydrophilic adhesives containing different PA concentrations were prepared by combining 50 wt% resin comonomer mixtures with 50 wt% ethanol. Proanthocyanidin was added to the ethanol-solvated resin to yield three adhesives with PA concentrations of 1.0, 2.0 and 3.0 wt%, respectively. A PA-free adhesive served as the control. Flat dentine surfaces from 40 extracted third molars were etched with 32% phosphoric acid. The specimens were randomly assigned to one of the four adhesive groups. Two layers of one of the four experimental adhesives were applied to the etched dentine and light-cured for 20 s. Composite build-ups were performed using Filtek Z250 (3M ESPE). After storage in distilled water at 37 °C for 24 h, twenty-four bonded teeth were sectioned into 0.9 mm × 0.9 mm beams and stressed to failure under tension for bond strength testing. Bond strength data were evaluated by one-way ANOVA and Tukey's test (α = 0.05). Interfacial nanoleakage was examined in the remaining teeth using a field-emission scanning electron microscope and analysed using the Chi-square test (α = 0.05). Results: No significant difference in bond strength was found amongst PA-free, 1% and 2% PA adhesives. However, incorporation of 3% PA into the adhesive significantly lowered bond strength as demonstrated by a greater number of adhesive failures and more extensive nanoleakage along the bonded interface. Conclusion: Incorporation of 2% proanthocyanidin into dental adhesives has no adverse effect on dentine bond strength. Clinical significance: The addition of proanthocyanidin to an experimental adhesive has no adverse effect on the immediate resin-dentine bond strength when the concentration of proanthocyanidin in the adhesive is less than or equal to 2%.

AB - Objectives: This study evaluated the effect of proanthocyanidin (PA) incorporation into experimental dental adhesives on resin-dentine bond strength. Methods: Four experimental hydrophilic adhesives containing different PA concentrations were prepared by combining 50 wt% resin comonomer mixtures with 50 wt% ethanol. Proanthocyanidin was added to the ethanol-solvated resin to yield three adhesives with PA concentrations of 1.0, 2.0 and 3.0 wt%, respectively. A PA-free adhesive served as the control. Flat dentine surfaces from 40 extracted third molars were etched with 32% phosphoric acid. The specimens were randomly assigned to one of the four adhesive groups. Two layers of one of the four experimental adhesives were applied to the etched dentine and light-cured for 20 s. Composite build-ups were performed using Filtek Z250 (3M ESPE). After storage in distilled water at 37 °C for 24 h, twenty-four bonded teeth were sectioned into 0.9 mm × 0.9 mm beams and stressed to failure under tension for bond strength testing. Bond strength data were evaluated by one-way ANOVA and Tukey's test (α = 0.05). Interfacial nanoleakage was examined in the remaining teeth using a field-emission scanning electron microscope and analysed using the Chi-square test (α = 0.05). Results: No significant difference in bond strength was found amongst PA-free, 1% and 2% PA adhesives. However, incorporation of 3% PA into the adhesive significantly lowered bond strength as demonstrated by a greater number of adhesive failures and more extensive nanoleakage along the bonded interface. Conclusion: Incorporation of 2% proanthocyanidin into dental adhesives has no adverse effect on dentine bond strength. Clinical significance: The addition of proanthocyanidin to an experimental adhesive has no adverse effect on the immediate resin-dentine bond strength when the concentration of proanthocyanidin in the adhesive is less than or equal to 2%.

KW - Bond strength

KW - Collagen cross-linking

KW - Dentine

KW - Proanthocyanidin

KW - Resin

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