TY - JOUR
T1 - Effect of rabbit anti-asialo-GM1 (GA1) polyclonal antibodies on neuromuscular transmission and acetylcholine-induced action potentials
T2 - Neurophysiological and immunohistochemical studies
AU - Taguchi, Kyoji
AU - Utsunomiya, Iku
AU - Ren, Jin
AU - Yoshida, Noriaki
AU - Aoyagi, Hiroyuki
AU - Nakatani, Yoshihiko
AU - Ariga, Toshio
AU - Usuki, Seigo
AU - Yu, Robert K.
AU - Miyatake, Tadashi
N1 - Funding Information:
This work was supported in part by a Research Grant for Neu-roimmunological Diseases from the Ministry of Health and Welfare of Japan, by Kimi Imai Memorial Foundation for research of Incurable Neuromuscular Diseases (to T. M.), and by USPHS Grant No. NS 26994 (to R. K. Y).
PY - 2004/5
Y1 - 2004/5
N2 - We produced anti-asialo-GM1 (GA1) polyclonal antibodies by sensitizing New Zealand rabbits with GA1 and investigated the epitopes and pathogenic role of anti-GA1 antibodies that appeared in serum. The serum blocked neuromuscular transmission, but not acetylcholine (ACh)-induced potentials, in muscle-spinal cord cocultured cells. The effect was complement independent. The antibodies inhibited voltage-gated Ca2+ channel (VGCC). The epitopes recognized by the antibodies were located in the outer membrane of Schwann cells and motor axons of Wistar rat ventral roots and on motor axons extended from spinal cord to muscle cells in muscle-spinal cocultured cells. The ACh-induced potential was not reduced by the addition of sera, suggesting the blockade is presynaptic. Thus, anti-GA1 antibodies may block neuromuscular transmission by suppressing VGCC on axonal terminals of motor nerves.
AB - We produced anti-asialo-GM1 (GA1) polyclonal antibodies by sensitizing New Zealand rabbits with GA1 and investigated the epitopes and pathogenic role of anti-GA1 antibodies that appeared in serum. The serum blocked neuromuscular transmission, but not acetylcholine (ACh)-induced potentials, in muscle-spinal cord cocultured cells. The effect was complement independent. The antibodies inhibited voltage-gated Ca2+ channel (VGCC). The epitopes recognized by the antibodies were located in the outer membrane of Schwann cells and motor axons of Wistar rat ventral roots and on motor axons extended from spinal cord to muscle cells in muscle-spinal cocultured cells. The ACh-induced potential was not reduced by the addition of sera, suggesting the blockade is presynaptic. Thus, anti-GA1 antibodies may block neuromuscular transmission by suppressing VGCC on axonal terminals of motor nerves.
KW - Anti-asialo-GM1 (GA1) antibodies
KW - Guillain-Barré syndrome
KW - Neuromuscular transmission block
KW - Voltage-gated Ca channel (VGCC)
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U2 - 10.1023/B:NERE.0000021239.86287.a3
DO - 10.1023/B:NERE.0000021239.86287.a3
M3 - Article
C2 - 15139293
AN - SCOPUS:3142742235
SN - 0364-3190
VL - 29
SP - 953
EP - 960
JO - Neurochemical Research
JF - Neurochemical Research
IS - 5
ER -