Effect of rabbit anti-asialo-GM1 (GA1) polyclonal antibodies on neuromuscular transmission and acetylcholine-induced action potentials

Neurophysiological and immunohistochemical studies

Kyoji Taguchi, Iku Utsunomiya, Jin Ren, Noriaki Yoshida, Hiroyuki Aoyagi, Yoshihiko Nakatani, Toshio Ariga, Seigo Usuki, Robert K Yu, Tadashi Miyatake

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We produced anti-asialo-GM1 (GA1) polyclonal antibodies by sensitizing New Zealand rabbits with GA1 and investigated the epitopes and pathogenic role of anti-GA1 antibodies that appeared in serum. The serum blocked neuromuscular transmission, but not acetylcholine (ACh)-induced potentials, in muscle-spinal cord cocultured cells. The effect was complement independent. The antibodies inhibited voltage-gated Ca2+ channel (VGCC). The epitopes recognized by the antibodies were located in the outer membrane of Schwann cells and motor axons of Wistar rat ventral roots and on motor axons extended from spinal cord to muscle cells in muscle-spinal cocultured cells. The ACh-induced potential was not reduced by the addition of sera, suggesting the blockade is presynaptic. Thus, anti-GA1 antibodies may block neuromuscular transmission by suppressing VGCC on axonal terminals of motor nerves.

Original languageEnglish (US)
Pages (from-to)953-960
Number of pages8
JournalNeurochemical Research
Volume29
Issue number5
DOIs
StatePublished - May 1 2004

Fingerprint

Action Potentials
Acetylcholine
Rabbits
Axons
Antibodies
Epitopes
Anti-Idiotypic Antibodies
Spinal Cord
Muscle
Serum
Muscles
Neuromuscular Blockade
Spinal Nerve Roots
Schwann Cells
Muscle Cells
Cells
Wistar Rats
Electric potential
Membranes
Rats

Keywords

  • Anti-asialo-GM1 (GA1) antibodies
  • Guillain-Barré syndrome
  • Neuromuscular transmission block
  • Voltage-gated Ca channel (VGCC)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Effect of rabbit anti-asialo-GM1 (GA1) polyclonal antibodies on neuromuscular transmission and acetylcholine-induced action potentials : Neurophysiological and immunohistochemical studies. / Taguchi, Kyoji; Utsunomiya, Iku; Ren, Jin; Yoshida, Noriaki; Aoyagi, Hiroyuki; Nakatani, Yoshihiko; Ariga, Toshio; Usuki, Seigo; Yu, Robert K; Miyatake, Tadashi.

In: Neurochemical Research, Vol. 29, No. 5, 01.05.2004, p. 953-960.

Research output: Contribution to journalArticle

Taguchi, Kyoji ; Utsunomiya, Iku ; Ren, Jin ; Yoshida, Noriaki ; Aoyagi, Hiroyuki ; Nakatani, Yoshihiko ; Ariga, Toshio ; Usuki, Seigo ; Yu, Robert K ; Miyatake, Tadashi. / Effect of rabbit anti-asialo-GM1 (GA1) polyclonal antibodies on neuromuscular transmission and acetylcholine-induced action potentials : Neurophysiological and immunohistochemical studies. In: Neurochemical Research. 2004 ; Vol. 29, No. 5. pp. 953-960.
@article{c74bccd3f5524a36a2238e4d68afe959,
title = "Effect of rabbit anti-asialo-GM1 (GA1) polyclonal antibodies on neuromuscular transmission and acetylcholine-induced action potentials: Neurophysiological and immunohistochemical studies",
abstract = "We produced anti-asialo-GM1 (GA1) polyclonal antibodies by sensitizing New Zealand rabbits with GA1 and investigated the epitopes and pathogenic role of anti-GA1 antibodies that appeared in serum. The serum blocked neuromuscular transmission, but not acetylcholine (ACh)-induced potentials, in muscle-spinal cord cocultured cells. The effect was complement independent. The antibodies inhibited voltage-gated Ca2+ channel (VGCC). The epitopes recognized by the antibodies were located in the outer membrane of Schwann cells and motor axons of Wistar rat ventral roots and on motor axons extended from spinal cord to muscle cells in muscle-spinal cocultured cells. The ACh-induced potential was not reduced by the addition of sera, suggesting the blockade is presynaptic. Thus, anti-GA1 antibodies may block neuromuscular transmission by suppressing VGCC on axonal terminals of motor nerves.",
keywords = "Anti-asialo-GM1 (GA1) antibodies, Guillain-Barr{\'e} syndrome, Neuromuscular transmission block, Voltage-gated Ca channel (VGCC)",
author = "Kyoji Taguchi and Iku Utsunomiya and Jin Ren and Noriaki Yoshida and Hiroyuki Aoyagi and Yoshihiko Nakatani and Toshio Ariga and Seigo Usuki and Yu, {Robert K} and Tadashi Miyatake",
year = "2004",
month = "5",
day = "1",
doi = "10.1023/B:NERE.0000021239.86287.a3",
language = "English (US)",
volume = "29",
pages = "953--960",
journal = "Neurochemical Research",
issn = "0364-3190",
publisher = "Springer New York",
number = "5",

}

TY - JOUR

T1 - Effect of rabbit anti-asialo-GM1 (GA1) polyclonal antibodies on neuromuscular transmission and acetylcholine-induced action potentials

T2 - Neurophysiological and immunohistochemical studies

AU - Taguchi, Kyoji

AU - Utsunomiya, Iku

AU - Ren, Jin

AU - Yoshida, Noriaki

AU - Aoyagi, Hiroyuki

AU - Nakatani, Yoshihiko

AU - Ariga, Toshio

AU - Usuki, Seigo

AU - Yu, Robert K

AU - Miyatake, Tadashi

PY - 2004/5/1

Y1 - 2004/5/1

N2 - We produced anti-asialo-GM1 (GA1) polyclonal antibodies by sensitizing New Zealand rabbits with GA1 and investigated the epitopes and pathogenic role of anti-GA1 antibodies that appeared in serum. The serum blocked neuromuscular transmission, but not acetylcholine (ACh)-induced potentials, in muscle-spinal cord cocultured cells. The effect was complement independent. The antibodies inhibited voltage-gated Ca2+ channel (VGCC). The epitopes recognized by the antibodies were located in the outer membrane of Schwann cells and motor axons of Wistar rat ventral roots and on motor axons extended from spinal cord to muscle cells in muscle-spinal cocultured cells. The ACh-induced potential was not reduced by the addition of sera, suggesting the blockade is presynaptic. Thus, anti-GA1 antibodies may block neuromuscular transmission by suppressing VGCC on axonal terminals of motor nerves.

AB - We produced anti-asialo-GM1 (GA1) polyclonal antibodies by sensitizing New Zealand rabbits with GA1 and investigated the epitopes and pathogenic role of anti-GA1 antibodies that appeared in serum. The serum blocked neuromuscular transmission, but not acetylcholine (ACh)-induced potentials, in muscle-spinal cord cocultured cells. The effect was complement independent. The antibodies inhibited voltage-gated Ca2+ channel (VGCC). The epitopes recognized by the antibodies were located in the outer membrane of Schwann cells and motor axons of Wistar rat ventral roots and on motor axons extended from spinal cord to muscle cells in muscle-spinal cocultured cells. The ACh-induced potential was not reduced by the addition of sera, suggesting the blockade is presynaptic. Thus, anti-GA1 antibodies may block neuromuscular transmission by suppressing VGCC on axonal terminals of motor nerves.

KW - Anti-asialo-GM1 (GA1) antibodies

KW - Guillain-Barré syndrome

KW - Neuromuscular transmission block

KW - Voltage-gated Ca channel (VGCC)

UR - http://www.scopus.com/inward/record.url?scp=3142742235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142742235&partnerID=8YFLogxK

U2 - 10.1023/B:NERE.0000021239.86287.a3

DO - 10.1023/B:NERE.0000021239.86287.a3

M3 - Article

VL - 29

SP - 953

EP - 960

JO - Neurochemical Research

JF - Neurochemical Research

SN - 0364-3190

IS - 5

ER -