Effect of removal of surface collagen fibrils on resin-dentin bonding

C. Prati, S. Chersoni, David Henry Pashley

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Objectives: The aim of this study was to evaluate the effects of NaOCl at removing the demineralized layer by examining the morphology of the hybrid layer and measuring shear bond strengths after different dentin treatments. Methods: Dentin disks were treated with: (1) 35% phosphoric acid (PA) 20 s; (2) PA treatment followed by 1.5% NaOCl, 2 min; (3) PA treatment, followed by a 10% NaOCl immersion for 120 h. SEM was used to analyze the morphology of dentin and its interface with dentin bonding agents (DBAs), while shear bond strength tests were used to measure adhesion. All specimens were then fractured into two halves: One half was inspected under SEM; the other half was sequentially placed in 10% PA followed by 12.5% NaOCl for 70 h, to remove all dentin from the resin replica of the original bonded interface. Results: SEM observations showed that collagen fibrils were completely removed from the acid-etched surface by NaOCl treatment. The diameter and the size of dentinal tubules and the number of lateral branches of the tubules were increased following NaOCl treatment. NaOCl applied on dentin smear layers did not significantly modify their SEM morphology. Resin tags were larger in diameter after phosphoric acid/ NaOCl treatment than after only phosphoric acid treatment. Resin-infiltrated dentin-layers were only observed after the single phosphoric acid (i.e. conventional etching) procedure, and were not observed after combined phosphoric acid/NaOCl treatment. Etched/NaOCl samples showed a lower bond strength using Scotchbond MP and 3M Single Bond, but were higher in Optibond FL and unmodified in Prime & Bond 2.0 groups when compared with acid-etched controls. Treatment of etched dentin with NaOCl for 120 h produced an unusual type of resin infiltration of mineralized dentin that could be called a "reverse hybrid layer" which may explain the mechanism of resin bonding to NaOCl treated dentin. Significance: The use of acidic conditioners for exposure of the collagen matrix exposes a soft delicate mesh that can collapse, thereby interfering with resin infiltration. If acid-etching is followed by NaOCl treatment, high bond strengths can be achieved via "reverse hybrid layer" formation, a proposed new mechanism of micromechanical resin retention. This mechanism is not yet recommended for clinical use but demonstrates a new type of resin retention.

Original languageEnglish (US)
Pages (from-to)323-331
Number of pages9
JournalDental Materials
Volume15
Issue number5
DOIs
StatePublished - Jan 1 1999

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Phosphoric acid
Dentin
Collagen
Resins
Scanning electron microscopy
Shear Strength
Infiltration
Acids
Etching
Dentin-Bonding Agents
Smear Layer
phosphoric acid
Bond strength (materials)
Immersion
Adhesion

Keywords

  • Dentine enamel
  • Dentinoenamel junction
  • Resin-infiltrated dentin-layers

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Effect of removal of surface collagen fibrils on resin-dentin bonding. / Prati, C.; Chersoni, S.; Pashley, David Henry.

In: Dental Materials, Vol. 15, No. 5, 01.01.1999, p. 323-331.

Research output: Contribution to journalArticle

Prati, C. ; Chersoni, S. ; Pashley, David Henry. / Effect of removal of surface collagen fibrils on resin-dentin bonding. In: Dental Materials. 1999 ; Vol. 15, No. 5. pp. 323-331.
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