Effect of stress and cyclosporine on ornithine decarboxylase activity in rat submaxillary lymph nodes

Ana I. Esquifino, Agustín Arce, Javier Eduardo Stern, Daniel P. Cardinali

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This study was performed: (1) to assess whether the stress produced in rats by daily turpentine oil injections for 5 days, or by restraining the animals for 30 min during 5 days, affected basal and Freund's adjuvant-stimulated submaxillary lymph node ornithine decarboxylase activity, an indicator of cell proliferation; (2) to analyze whether the activity of the immunosuppressive drug cyclosporine on submaxillary lymph node ornithine decarboxylase activation after Freund's adjuvant injection was modified in stressed rats; (3) to examine the mediation of stress effects on submaxillary lymph node ornithine decarboxylase activation by regional sympathetic or parasympathetic nerves. Animals subjected to a unilateral superior cervical ganglionectomy, or to a unilateral chorda tympani section, together with a contralateral sham-operation were employed. After turpentine oil or restraint stress, a significant decrease in submaxillary lymph node ornithine decarboxylase was found. A unilateral sympathetic denervation of submaxillary lymph nodes counteracted in part the inhibitory effect of stress on ornithine decarboxylase activation, as well as augmented the enzyme response in innervated submaxillary lymph nodes. Ornithine decarboxylase activation attained similar values in parasympathetic decentralized or intact submaxillary lymph nodes and the unilateral parasympathetic decentralization did not interfere with the inhibition of enzyme activity found in turpentine oil-stressed rats. Cyclosporine administration (5 or 20 mg/kg) significantly decreased Freund's adjuvant-induced ornithine decarboxylase activity in the submaxillary lymph nodes of control rats, but failed to modify it in turpentine oil-stressed animals. In this latter group, a higher (40 mg/kg) dose of cyclosporine decreased ornithine decarboxylase activity on the innervated side only. A diminished inhibitory response to cyclosporine was found in the parasympathetic decentralized submaxillary lymph nodes of unstressed rats. The results support the view that the immunosuppressive effects of cyclosporine may diminish during stress, in part due to changes in the traffic of neural signals in local sympathetic nerves.

Original languageEnglish (US)
Pages (from-to)143-149
Number of pages7
JournalEuropean Journal of Pharmacology
Volume285
Issue number2
DOIs
StatePublished - Oct 16 1995

Fingerprint

Ornithine Decarboxylase
Cyclosporine
Lymph Nodes
Turpentine
Freund's Adjuvant
Immunosuppressive Agents
Ganglionectomy
Injections
Sympathectomy
Enzymes
Politics
Cell Proliferation

Keywords

  • Chorda tympani
  • Cyclosporine
  • Neuroimmunomodulation
  • Parasympathetic nervous system
  • Stress
  • Submaxillary lymph node
  • Superior cervical ganglion
  • Sympathetic nervous system

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effect of stress and cyclosporine on ornithine decarboxylase activity in rat submaxillary lymph nodes. / Esquifino, Ana I.; Arce, Agustín; Stern, Javier Eduardo; Cardinali, Daniel P.

In: European Journal of Pharmacology, Vol. 285, No. 2, 16.10.1995, p. 143-149.

Research output: Contribution to journalArticle

Esquifino, Ana I. ; Arce, Agustín ; Stern, Javier Eduardo ; Cardinali, Daniel P. / Effect of stress and cyclosporine on ornithine decarboxylase activity in rat submaxillary lymph nodes. In: European Journal of Pharmacology. 1995 ; Vol. 285, No. 2. pp. 143-149.
@article{ba55ca92809b4cf185e51bb230674924,
title = "Effect of stress and cyclosporine on ornithine decarboxylase activity in rat submaxillary lymph nodes",
abstract = "This study was performed: (1) to assess whether the stress produced in rats by daily turpentine oil injections for 5 days, or by restraining the animals for 30 min during 5 days, affected basal and Freund's adjuvant-stimulated submaxillary lymph node ornithine decarboxylase activity, an indicator of cell proliferation; (2) to analyze whether the activity of the immunosuppressive drug cyclosporine on submaxillary lymph node ornithine decarboxylase activation after Freund's adjuvant injection was modified in stressed rats; (3) to examine the mediation of stress effects on submaxillary lymph node ornithine decarboxylase activation by regional sympathetic or parasympathetic nerves. Animals subjected to a unilateral superior cervical ganglionectomy, or to a unilateral chorda tympani section, together with a contralateral sham-operation were employed. After turpentine oil or restraint stress, a significant decrease in submaxillary lymph node ornithine decarboxylase was found. A unilateral sympathetic denervation of submaxillary lymph nodes counteracted in part the inhibitory effect of stress on ornithine decarboxylase activation, as well as augmented the enzyme response in innervated submaxillary lymph nodes. Ornithine decarboxylase activation attained similar values in parasympathetic decentralized or intact submaxillary lymph nodes and the unilateral parasympathetic decentralization did not interfere with the inhibition of enzyme activity found in turpentine oil-stressed rats. Cyclosporine administration (5 or 20 mg/kg) significantly decreased Freund's adjuvant-induced ornithine decarboxylase activity in the submaxillary lymph nodes of control rats, but failed to modify it in turpentine oil-stressed animals. In this latter group, a higher (40 mg/kg) dose of cyclosporine decreased ornithine decarboxylase activity on the innervated side only. A diminished inhibitory response to cyclosporine was found in the parasympathetic decentralized submaxillary lymph nodes of unstressed rats. The results support the view that the immunosuppressive effects of cyclosporine may diminish during stress, in part due to changes in the traffic of neural signals in local sympathetic nerves.",
keywords = "Chorda tympani, Cyclosporine, Neuroimmunomodulation, Parasympathetic nervous system, Stress, Submaxillary lymph node, Superior cervical ganglion, Sympathetic nervous system",
author = "Esquifino, {Ana I.} and Agust{\'i}n Arce and Stern, {Javier Eduardo} and Cardinali, {Daniel P.}",
year = "1995",
month = "10",
day = "16",
doi = "10.1016/0014-2999(95)00396-3",
language = "English (US)",
volume = "285",
pages = "143--149",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Effect of stress and cyclosporine on ornithine decarboxylase activity in rat submaxillary lymph nodes

AU - Esquifino, Ana I.

AU - Arce, Agustín

AU - Stern, Javier Eduardo

AU - Cardinali, Daniel P.

PY - 1995/10/16

Y1 - 1995/10/16

N2 - This study was performed: (1) to assess whether the stress produced in rats by daily turpentine oil injections for 5 days, or by restraining the animals for 30 min during 5 days, affected basal and Freund's adjuvant-stimulated submaxillary lymph node ornithine decarboxylase activity, an indicator of cell proliferation; (2) to analyze whether the activity of the immunosuppressive drug cyclosporine on submaxillary lymph node ornithine decarboxylase activation after Freund's adjuvant injection was modified in stressed rats; (3) to examine the mediation of stress effects on submaxillary lymph node ornithine decarboxylase activation by regional sympathetic or parasympathetic nerves. Animals subjected to a unilateral superior cervical ganglionectomy, or to a unilateral chorda tympani section, together with a contralateral sham-operation were employed. After turpentine oil or restraint stress, a significant decrease in submaxillary lymph node ornithine decarboxylase was found. A unilateral sympathetic denervation of submaxillary lymph nodes counteracted in part the inhibitory effect of stress on ornithine decarboxylase activation, as well as augmented the enzyme response in innervated submaxillary lymph nodes. Ornithine decarboxylase activation attained similar values in parasympathetic decentralized or intact submaxillary lymph nodes and the unilateral parasympathetic decentralization did not interfere with the inhibition of enzyme activity found in turpentine oil-stressed rats. Cyclosporine administration (5 or 20 mg/kg) significantly decreased Freund's adjuvant-induced ornithine decarboxylase activity in the submaxillary lymph nodes of control rats, but failed to modify it in turpentine oil-stressed animals. In this latter group, a higher (40 mg/kg) dose of cyclosporine decreased ornithine decarboxylase activity on the innervated side only. A diminished inhibitory response to cyclosporine was found in the parasympathetic decentralized submaxillary lymph nodes of unstressed rats. The results support the view that the immunosuppressive effects of cyclosporine may diminish during stress, in part due to changes in the traffic of neural signals in local sympathetic nerves.

AB - This study was performed: (1) to assess whether the stress produced in rats by daily turpentine oil injections for 5 days, or by restraining the animals for 30 min during 5 days, affected basal and Freund's adjuvant-stimulated submaxillary lymph node ornithine decarboxylase activity, an indicator of cell proliferation; (2) to analyze whether the activity of the immunosuppressive drug cyclosporine on submaxillary lymph node ornithine decarboxylase activation after Freund's adjuvant injection was modified in stressed rats; (3) to examine the mediation of stress effects on submaxillary lymph node ornithine decarboxylase activation by regional sympathetic or parasympathetic nerves. Animals subjected to a unilateral superior cervical ganglionectomy, or to a unilateral chorda tympani section, together with a contralateral sham-operation were employed. After turpentine oil or restraint stress, a significant decrease in submaxillary lymph node ornithine decarboxylase was found. A unilateral sympathetic denervation of submaxillary lymph nodes counteracted in part the inhibitory effect of stress on ornithine decarboxylase activation, as well as augmented the enzyme response in innervated submaxillary lymph nodes. Ornithine decarboxylase activation attained similar values in parasympathetic decentralized or intact submaxillary lymph nodes and the unilateral parasympathetic decentralization did not interfere with the inhibition of enzyme activity found in turpentine oil-stressed rats. Cyclosporine administration (5 or 20 mg/kg) significantly decreased Freund's adjuvant-induced ornithine decarboxylase activity in the submaxillary lymph nodes of control rats, but failed to modify it in turpentine oil-stressed animals. In this latter group, a higher (40 mg/kg) dose of cyclosporine decreased ornithine decarboxylase activity on the innervated side only. A diminished inhibitory response to cyclosporine was found in the parasympathetic decentralized submaxillary lymph nodes of unstressed rats. The results support the view that the immunosuppressive effects of cyclosporine may diminish during stress, in part due to changes in the traffic of neural signals in local sympathetic nerves.

KW - Chorda tympani

KW - Cyclosporine

KW - Neuroimmunomodulation

KW - Parasympathetic nervous system

KW - Stress

KW - Submaxillary lymph node

KW - Superior cervical ganglion

KW - Sympathetic nervous system

UR - http://www.scopus.com/inward/record.url?scp=0028868452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028868452&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(95)00396-3

DO - 10.1016/0014-2999(95)00396-3

M3 - Article

VL - 285

SP - 143

EP - 149

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 2

ER -