Effect of UVA-activated riboflavin on dentin bonding

A. Cova, L. Breschi, F. Nato, A. Ruggeri, M. Carrilho, L. Tjäderhane, C. Prati, R. Di Lenarda, Franklin Chi Meng Tay, David Henry Pashley, A. Mazzoni

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds.

Original languageEnglish (US)
Pages (from-to)1439-1445
Number of pages7
JournalJournal of Dental Research
Volume90
Issue number12
DOIs
StatePublished - Dec 1 2011

Fingerprint

Riboflavin
Dentin
Matrix Metalloproteinases
Adhesives
Collagen
Transmission Electron Microscopy
Powders
Light

Keywords

  • collagen cross-linking
  • dentin bonding agent
  • dentin matrix
  • matrix metalloproteinases
  • nanoleakage
  • riboflavin

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Cova, A., Breschi, L., Nato, F., Ruggeri, A., Carrilho, M., Tjäderhane, L., ... Mazzoni, A. (2011). Effect of UVA-activated riboflavin on dentin bonding. Journal of Dental Research, 90(12), 1439-1445. https://doi.org/10.1177/0022034511423397

Effect of UVA-activated riboflavin on dentin bonding. / Cova, A.; Breschi, L.; Nato, F.; Ruggeri, A.; Carrilho, M.; Tjäderhane, L.; Prati, C.; Di Lenarda, R.; Tay, Franklin Chi Meng; Pashley, David Henry; Mazzoni, A.

In: Journal of Dental Research, Vol. 90, No. 12, 01.12.2011, p. 1439-1445.

Research output: Contribution to journalArticle

Cova, A, Breschi, L, Nato, F, Ruggeri, A, Carrilho, M, Tjäderhane, L, Prati, C, Di Lenarda, R, Tay, FCM, Pashley, DH & Mazzoni, A 2011, 'Effect of UVA-activated riboflavin on dentin bonding', Journal of Dental Research, vol. 90, no. 12, pp. 1439-1445. https://doi.org/10.1177/0022034511423397
Cova A, Breschi L, Nato F, Ruggeri A, Carrilho M, Tjäderhane L et al. Effect of UVA-activated riboflavin on dentin bonding. Journal of Dental Research. 2011 Dec 1;90(12):1439-1445. https://doi.org/10.1177/0022034511423397
Cova, A. ; Breschi, L. ; Nato, F. ; Ruggeri, A. ; Carrilho, M. ; Tjäderhane, L. ; Prati, C. ; Di Lenarda, R. ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Mazzoni, A. / Effect of UVA-activated riboflavin on dentin bonding. In: Journal of Dental Research. 2011 ; Vol. 90, No. 12. pp. 1439-1445.
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AU - Prati, C.

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