Effects of different antigenic microenvironments on the course of CD8+ T cell responses in vivo

Raquel Tarazona, Anna Marit Sponaas, Georgia Mavria, Min Zhou, Ruth Schulz, Peter Tomlinson, Jane Antoniou, Andrew L. Mellor

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The influence of microenvironment on the course of CD8+ T cell responses in vivo was investigated by injecting H-2Kb-specific T cells from donor TCR transgenic (TCR-Tg) mice into H-2Kb-Tg mice. H-2Kb expression in recipients was either ubiquitous (CBK mice) or restricted to myeloid and erythroid cells (Kβ mice). Donor T cells proliferated as extensively and acquired similar surface phenotypes in spleen of both recipient types. Thus, neither the restricted pattern of H-2Kb expression nor the significantly reduced level of H-2Kb expression by myeloid cells in Kβ recipients affects the ability of the splenic microenvironment to prime T cell proliferation in vivo. However, an unsustained burst of cytolytic activity was generated rapidly in spleen of CBK recipients, whereas relatively little cytolytic activity was generated in Kβ spleen. This indicates that effector T cells were not generated efficiently in spleen of Kβ recipients even though extensive T cell proliferation was taking place in this microenvironment. Furthermore, activated donor T cells dispersed rapidly throughout primary and secondary lymphoid organs of Kβ recipients, whereas few T cells migrated from spleen in CBK recipients. Consequently, the course of CD8+ T cell responses and the anatomical distribution of activated T cells are profoundly influenced by the nature of the antigenic microenvironment encountered in vivo. We conclude that T cells rapidly proliferate and acquire new tissue-homing characteristics but do not differentiate into cytolytic effector cells at the site of priming when they encounter myeloid cells expressing low levels of antigen in vivo.

Original languageEnglish (US)
Pages (from-to)351-358
Number of pages8
JournalInternational Immunology
Volume8
Issue number3
DOIs
StatePublished - 1996

Keywords

  • Activation
  • Cytotoxicity
  • Differentiation
  • Proliferation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Tarazona, R., Sponaas, A. M., Mavria, G., Zhou, M., Schulz, R., Tomlinson, P., Antoniou, J., & Mellor, A. L. (1996). Effects of different antigenic microenvironments on the course of CD8+ T cell responses in vivo. International Immunology, 8(3), 351-358. https://doi.org/10.1093/intimm/8.3.351