Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9

A. Mazzoni, P. Scaffa, M. Carrilho, L. Tjäderhane, R. Di Lenarda, A. Polimeni, A. Tezvergil-Mutluay, Franklin Chi Meng Tay, David Henry Pashley, L. Breschi

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Auto-degradation of collagen matrices occurs within hybrid layers created by contemporary dentin bonding systems, by the slow action of host-derived matrix metalloproteinases (MMPs). This study tested the null hypothesis that there are no differences in the activities of MMP-2 and -9 after treatment with different etch-and-rinse or self-etch adhesives. Tested adhesives were: Adper Scotchbond 1XT (3M ESPE), PQ1 (Ultradent), Peak LC (Ultradent), Optibond Solo Plus (Kerr), Prime&Bond NT (Dentsply) (all 2-step etch-and-rinse adhesives), and Adper Easy Bond (3M ESPE), Tri-S (Kuraray), and Xeno-V (Dentsply) (1-step self-etch adhesives). MMP-2 and -9 activities were quantified in adhesive-treated dentin powder by means of an activity assay and gelatin zymography. MMP-2 and MMP-9 activities were found after treatment with all of the simplified etch-and-rinse and self-etch adhesives; however, the activation was adhesive-dependent. It is concluded that all two-step etch-and-rinse and the one-step self-etch adhesives tested can activate endogenous MMP-2 and MMP-9 in human dentin. These results support the role of endogenous MMPs in the degradation of hybrid layers created by these adhesives.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalJournal of Dental Research
Volume92
Issue number1
DOIs
StatePublished - Jan 1 2013

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Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Dentin
Adhesives
Matrix Metalloproteinases
Gelatin
Powders
Collagen

Keywords

  • auto-degradation
  • biochemical assays
  • collagen
  • dentin bonding systems
  • hybrid layer
  • non-collageneous proteins

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Mazzoni, A., Scaffa, P., Carrilho, M., Tjäderhane, L., Di Lenarda, R., Polimeni, A., ... Breschi, L. (2013). Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. Journal of Dental Research, 92(1), 82-86. https://doi.org/10.1177/0022034512467034

Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. / Mazzoni, A.; Scaffa, P.; Carrilho, M.; Tjäderhane, L.; Di Lenarda, R.; Polimeni, A.; Tezvergil-Mutluay, A.; Tay, Franklin Chi Meng; Pashley, David Henry; Breschi, L.

In: Journal of Dental Research, Vol. 92, No. 1, 01.01.2013, p. 82-86.

Research output: Contribution to journalArticle

Mazzoni, A, Scaffa, P, Carrilho, M, Tjäderhane, L, Di Lenarda, R, Polimeni, A, Tezvergil-Mutluay, A, Tay, FCM, Pashley, DH & Breschi, L 2013, 'Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9', Journal of Dental Research, vol. 92, no. 1, pp. 82-86. https://doi.org/10.1177/0022034512467034
Mazzoni A, Scaffa P, Carrilho M, Tjäderhane L, Di Lenarda R, Polimeni A et al. Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. Journal of Dental Research. 2013 Jan 1;92(1):82-86. https://doi.org/10.1177/0022034512467034
Mazzoni, A. ; Scaffa, P. ; Carrilho, M. ; Tjäderhane, L. ; Di Lenarda, R. ; Polimeni, A. ; Tezvergil-Mutluay, A. ; Tay, Franklin Chi Meng ; Pashley, David Henry ; Breschi, L. / Effects of etch-and-rinse and self-etch adhesives on dentin MMP-2 and MMP-9. In: Journal of Dental Research. 2013 ; Vol. 92, No. 1. pp. 82-86.
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