Effects of extracellular Na+ and Ca2+ ions and Ca2+ channel modulators on the cell-associated activity of 99mTc-MIBI and 99mTc-tetrofosmin in tumour cells

Ali Syed Arbab, J. Ueki, K. Koizumi, T. Araki

Research output: Contribution to journalArticle

Abstract

Our aim was to determine whether the Ca2+ ion or cell membrane Ca2+ and Na+/Ca2+ ion transport systems are involved in maintaining the cell-associated activity of technetium-99m-hexakis- methoxyisobutyl-isonitrile (99mTc-MIBI) and technetium-99m-ethylene- bis[bis(2-ethoxyethyl)phosphin] (99mTc-tetrofosmin) in tumour cell lines. The cell-associated activities of 99mTc-MIBI and 99mTc-tetrofosmin were assessed in various buffers, with or without Na+ and/or with different concentrations of Ca2+, in Lewi's murine lung cell carcinoma and human glioma cell lines. Different Ca 2+ channel modulators, such as verapamil, flunarizine and 3,4-dichlorobenzamil (DCB), were used to assess the effect of Ca2+ channels on the cell-associated activity of 99mTc-MIBI and 99mTc-tetrofosmin. Despite significant differences between cell lines, the cell-associated activity of 99mTc-MIBI was higher in buffers without extracellular Ca2+ and Na+. The cell-associated activity of 99mTc-MIBI was significantly lower in all buffers containing high concentrations of Ca2+ in both cell lines. The cell-associated activity of 99mTc-tetrofosmin was also significantly higher in buffers without Ca2+, and was significantly decreased in buffers with high concentrations of Ca2+. All modulators significantly increased the cell-associated activity of 99mTc-MIBI in both cell lines in all buffers. All modulators increased the cell-associated activity of 99mTc-tetrofosmin, particularly in buffers containing Ca2+. The cell-associated activities of both 99mTc-MIBI and 99mTctetrofosmin may be dependent on verapamil-, flunarizine- and DCB-sensitive Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)155-166
Number of pages12
JournalNuclear Medicine Communications
Volume24
Issue number2
DOIs
StatePublished - Feb 1 2003

Fingerprint

Ion Channels
Buffers
Neoplasms
Flunarizine
Cell Line
Technetium
Verapamil
technetium Tc 99m 1,2-bis(bis(2-ethoxyethyl)phosphino)ethane
Ion Transport
Tumor Cell Line
Glioma
Cell Membrane
Ions
Carcinoma
Lung

Keywords

  • Ca channels
  • Dichlorobenzamil
  • Flunarizine
  • Na channels
  • Tc-tetrofosmin
  • Verapamil

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Effects of extracellular Na+ and Ca2+ ions and Ca2+ channel modulators on the cell-associated activity of 99mTc-MIBI and 99mTc-tetrofosmin in tumour cells. / Arbab, Ali Syed; Ueki, J.; Koizumi, K.; Araki, T.

In: Nuclear Medicine Communications, Vol. 24, No. 2, 01.02.2003, p. 155-166.

Research output: Contribution to journalArticle

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AU - Koizumi, K.

AU - Araki, T.

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AB - Our aim was to determine whether the Ca2+ ion or cell membrane Ca2+ and Na+/Ca2+ ion transport systems are involved in maintaining the cell-associated activity of technetium-99m-hexakis- methoxyisobutyl-isonitrile (99mTc-MIBI) and technetium-99m-ethylene- bis[bis(2-ethoxyethyl)phosphin] (99mTc-tetrofosmin) in tumour cell lines. The cell-associated activities of 99mTc-MIBI and 99mTc-tetrofosmin were assessed in various buffers, with or without Na+ and/or with different concentrations of Ca2+, in Lewi's murine lung cell carcinoma and human glioma cell lines. Different Ca 2+ channel modulators, such as verapamil, flunarizine and 3,4-dichlorobenzamil (DCB), were used to assess the effect of Ca2+ channels on the cell-associated activity of 99mTc-MIBI and 99mTc-tetrofosmin. Despite significant differences between cell lines, the cell-associated activity of 99mTc-MIBI was higher in buffers without extracellular Ca2+ and Na+. The cell-associated activity of 99mTc-MIBI was significantly lower in all buffers containing high concentrations of Ca2+ in both cell lines. The cell-associated activity of 99mTc-tetrofosmin was also significantly higher in buffers without Ca2+, and was significantly decreased in buffers with high concentrations of Ca2+. All modulators significantly increased the cell-associated activity of 99mTc-MIBI in both cell lines in all buffers. All modulators increased the cell-associated activity of 99mTc-tetrofosmin, particularly in buffers containing Ca2+. The cell-associated activities of both 99mTc-MIBI and 99mTctetrofosmin may be dependent on verapamil-, flunarizine- and DCB-sensitive Ca2+ channels.

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