[Effects of hypoxia on proliferation of pulmonary arterial smooth muscle cells].

Tianzheng Yu, C. T. Ma

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To investigate the effects of hypoxia on the proliferation of different diameter intra-pulmonary artery smooth muscle cells (PASMCs), and to study the possible signal transduction pathway in proliferation caused by hypoxia. PASMCs were isolated from three different size of pulmonary arteries (>1 000 microm, 500-800 microm, 300-400 microm diameter) and cultured separately. 3H-TdR incorporation and cell number were used to measure cell proliferation. 3H-TdR incorporation and cell number of PASMCs from three sizes of pulmonary artery (> 1 000 microm, 500-800 microm, 800-400 microm diameter) increased 23.5% and 11.1%, 60.0% and 33.8%, 141.4% and 52.0%, respectively. Calcium antagonist (verapamil), PKC inhibitor (staurosporine), and Na(+)-H+ exchange inhibitor (amiloride) were used in PASMCs isolated from 300-400 microm diameter pulmonary artery. The results showed that verapamil, staurosporine and amiloride could notably block hypoxia-induced increase 3H-TdR incorporation and cell number. Proliferation of pulmonary artery smooth muscle cell responded to hypoxia differently according to the artery size; activation of calcium channel, PKC and Na(+)-H+ exchange might mediate the proliferation initiated by hypoxia.

Original languageEnglish (US)
Pages (from-to)58-60
Number of pages3
JournalZhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology
Volume17
Issue number1
StatePublished - Jan 1 2001

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Pulmonary Artery
Smooth Muscle Myocytes
Lung
Staurosporine
Cell Count
Amiloride
Verapamil
Hypoxia
Calcium Channels
Cell Size
Signal Transduction
Arteries
Cell Proliferation
Calcium

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "[Effects of hypoxia on proliferation of pulmonary arterial smooth muscle cells].",
abstract = "To investigate the effects of hypoxia on the proliferation of different diameter intra-pulmonary artery smooth muscle cells (PASMCs), and to study the possible signal transduction pathway in proliferation caused by hypoxia. PASMCs were isolated from three different size of pulmonary arteries (>1 000 microm, 500-800 microm, 300-400 microm diameter) and cultured separately. 3H-TdR incorporation and cell number were used to measure cell proliferation. 3H-TdR incorporation and cell number of PASMCs from three sizes of pulmonary artery (> 1 000 microm, 500-800 microm, 800-400 microm diameter) increased 23.5{\%} and 11.1{\%}, 60.0{\%} and 33.8{\%}, 141.4{\%} and 52.0{\%}, respectively. Calcium antagonist (verapamil), PKC inhibitor (staurosporine), and Na(+)-H+ exchange inhibitor (amiloride) were used in PASMCs isolated from 300-400 microm diameter pulmonary artery. The results showed that verapamil, staurosporine and amiloride could notably block hypoxia-induced increase 3H-TdR incorporation and cell number. Proliferation of pulmonary artery smooth muscle cell responded to hypoxia differently according to the artery size; activation of calcium channel, PKC and Na(+)-H+ exchange might mediate the proliferation initiated by hypoxia.",
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AB - To investigate the effects of hypoxia on the proliferation of different diameter intra-pulmonary artery smooth muscle cells (PASMCs), and to study the possible signal transduction pathway in proliferation caused by hypoxia. PASMCs were isolated from three different size of pulmonary arteries (>1 000 microm, 500-800 microm, 300-400 microm diameter) and cultured separately. 3H-TdR incorporation and cell number were used to measure cell proliferation. 3H-TdR incorporation and cell number of PASMCs from three sizes of pulmonary artery (> 1 000 microm, 500-800 microm, 800-400 microm diameter) increased 23.5% and 11.1%, 60.0% and 33.8%, 141.4% and 52.0%, respectively. Calcium antagonist (verapamil), PKC inhibitor (staurosporine), and Na(+)-H+ exchange inhibitor (amiloride) were used in PASMCs isolated from 300-400 microm diameter pulmonary artery. The results showed that verapamil, staurosporine and amiloride could notably block hypoxia-induced increase 3H-TdR incorporation and cell number. Proliferation of pulmonary artery smooth muscle cell responded to hypoxia differently according to the artery size; activation of calcium channel, PKC and Na(+)-H+ exchange might mediate the proliferation initiated by hypoxia.

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