Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells

J. A. Davidson, J. G. Betts, U. Tiemann, L. A. Kamwanja, Victor Hugo Monterroso, P. J. Hansen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ruminant conceptus secretes a unique interferon, interferon-τ, that regulates endometrial prostaglandin secretion during early pregnancy. Because one of the pleiotropic effects of interferons is to inhibit cellular proliferation, a series of experiments was conducted to determine whether or not the bovine endometrium is sensitive to the antiproliferative effect of interferon-τ and the related interferon, interferon-α. Endometrial epithelial and stromal cells were prepared from the endometrium of cows from Days 11-17 after estrus and incubated with recombinant bovine interferon-τ (rbIFNτ; 1-1000 ng/ml), recombinant bovine interferon-α1 (rbIFNα; 1-1000 ng/ml), recombinant human interferon-α2b (rhIFNα; 100 ng/ml), or ovine interferon-τ (oIFNτ; 100 ng/ml). Proliferation was determined by monitoring uptake of [3H]thymidine into DNA. Generally, interferons did not inhibit proliferation of endometrial epithelial cells. Exceptions were for 1000 ng/ml rbIFNτ, which inhibited proliferation by 23%; 100 ng/ml rbIFNα, which inhibited proliferation by 28% in one of two experiments only; and 100 ng/ml oIFNτ, which inhibited proliferation by 17%. Proliferation of endometrial stromal cells was not inhibited by any concentration of any interferon in two separate experiments. Therefore, unlike other bovine cells tested previously (lymphocytes and oviductal cells), bovine endometrial cells were not consistently inhibited by IFNτ or IFNα. Such reduced responsiveness of endometrial cells to the antiproliferative effects of type 1 interferons could allow for growth of the endometrium during the period of pregnancy when the conceptus produces IFNτ.

Original languageEnglish (US)
Pages (from-to)700-705
Number of pages6
JournalBiology of reproduction
Volume51
Issue number4
DOIs
StatePublished - Jan 1 1994

Fingerprint

Interferons
Endometrium
Stromal Cells
Epithelial Cells
Pregnancy
Interferon Type I
Estrus
Ruminants
Thymidine
Prostaglandins
Sheep
Cell Proliferation
Lymphocytes
DNA

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology

Cite this

Davidson, J. A., Betts, J. G., Tiemann, U., Kamwanja, L. A., Monterroso, V. H., & Hansen, P. J. (1994). Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells. Biology of reproduction, 51(4), 700-705. https://doi.org/10.1095/biolreprod51.4.700

Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells. / Davidson, J. A.; Betts, J. G.; Tiemann, U.; Kamwanja, L. A.; Monterroso, Victor Hugo; Hansen, P. J.

In: Biology of reproduction, Vol. 51, No. 4, 01.01.1994, p. 700-705.

Research output: Contribution to journalArticle

Davidson, JA, Betts, JG, Tiemann, U, Kamwanja, LA, Monterroso, VH & Hansen, PJ 1994, 'Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells', Biology of reproduction, vol. 51, no. 4, pp. 700-705. https://doi.org/10.1095/biolreprod51.4.700
Davidson JA, Betts JG, Tiemann U, Kamwanja LA, Monterroso VH, Hansen PJ. Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells. Biology of reproduction. 1994 Jan 1;51(4):700-705. https://doi.org/10.1095/biolreprod51.4.700
Davidson, J. A. ; Betts, J. G. ; Tiemann, U. ; Kamwanja, L. A. ; Monterroso, Victor Hugo ; Hansen, P. J. / Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells. In: Biology of reproduction. 1994 ; Vol. 51, No. 4. pp. 700-705.
@article{556dd3e4445249b697be5f677ccbbccf,
title = "Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells",
abstract = "The ruminant conceptus secretes a unique interferon, interferon-τ, that regulates endometrial prostaglandin secretion during early pregnancy. Because one of the pleiotropic effects of interferons is to inhibit cellular proliferation, a series of experiments was conducted to determine whether or not the bovine endometrium is sensitive to the antiproliferative effect of interferon-τ and the related interferon, interferon-α. Endometrial epithelial and stromal cells were prepared from the endometrium of cows from Days 11-17 after estrus and incubated with recombinant bovine interferon-τ (rbIFNτ; 1-1000 ng/ml), recombinant bovine interferon-α1 (rbIFNα; 1-1000 ng/ml), recombinant human interferon-α2b (rhIFNα; 100 ng/ml), or ovine interferon-τ (oIFNτ; 100 ng/ml). Proliferation was determined by monitoring uptake of [3H]thymidine into DNA. Generally, interferons did not inhibit proliferation of endometrial epithelial cells. Exceptions were for 1000 ng/ml rbIFNτ, which inhibited proliferation by 23{\%}; 100 ng/ml rbIFNα, which inhibited proliferation by 28{\%} in one of two experiments only; and 100 ng/ml oIFNτ, which inhibited proliferation by 17{\%}. Proliferation of endometrial stromal cells was not inhibited by any concentration of any interferon in two separate experiments. Therefore, unlike other bovine cells tested previously (lymphocytes and oviductal cells), bovine endometrial cells were not consistently inhibited by IFNτ or IFNα. Such reduced responsiveness of endometrial cells to the antiproliferative effects of type 1 interferons could allow for growth of the endometrium during the period of pregnancy when the conceptus produces IFNτ.",
author = "Davidson, {J. A.} and Betts, {J. G.} and U. Tiemann and Kamwanja, {L. A.} and Monterroso, {Victor Hugo} and Hansen, {P. J.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1095/biolreprod51.4.700",
language = "English (US)",
volume = "51",
pages = "700--705",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "4",

}

TY - JOUR

T1 - Effects of interferon-τ and interferon-α on proliferation of bovine endometrial cells

AU - Davidson, J. A.

AU - Betts, J. G.

AU - Tiemann, U.

AU - Kamwanja, L. A.

AU - Monterroso, Victor Hugo

AU - Hansen, P. J.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The ruminant conceptus secretes a unique interferon, interferon-τ, that regulates endometrial prostaglandin secretion during early pregnancy. Because one of the pleiotropic effects of interferons is to inhibit cellular proliferation, a series of experiments was conducted to determine whether or not the bovine endometrium is sensitive to the antiproliferative effect of interferon-τ and the related interferon, interferon-α. Endometrial epithelial and stromal cells were prepared from the endometrium of cows from Days 11-17 after estrus and incubated with recombinant bovine interferon-τ (rbIFNτ; 1-1000 ng/ml), recombinant bovine interferon-α1 (rbIFNα; 1-1000 ng/ml), recombinant human interferon-α2b (rhIFNα; 100 ng/ml), or ovine interferon-τ (oIFNτ; 100 ng/ml). Proliferation was determined by monitoring uptake of [3H]thymidine into DNA. Generally, interferons did not inhibit proliferation of endometrial epithelial cells. Exceptions were for 1000 ng/ml rbIFNτ, which inhibited proliferation by 23%; 100 ng/ml rbIFNα, which inhibited proliferation by 28% in one of two experiments only; and 100 ng/ml oIFNτ, which inhibited proliferation by 17%. Proliferation of endometrial stromal cells was not inhibited by any concentration of any interferon in two separate experiments. Therefore, unlike other bovine cells tested previously (lymphocytes and oviductal cells), bovine endometrial cells were not consistently inhibited by IFNτ or IFNα. Such reduced responsiveness of endometrial cells to the antiproliferative effects of type 1 interferons could allow for growth of the endometrium during the period of pregnancy when the conceptus produces IFNτ.

AB - The ruminant conceptus secretes a unique interferon, interferon-τ, that regulates endometrial prostaglandin secretion during early pregnancy. Because one of the pleiotropic effects of interferons is to inhibit cellular proliferation, a series of experiments was conducted to determine whether or not the bovine endometrium is sensitive to the antiproliferative effect of interferon-τ and the related interferon, interferon-α. Endometrial epithelial and stromal cells were prepared from the endometrium of cows from Days 11-17 after estrus and incubated with recombinant bovine interferon-τ (rbIFNτ; 1-1000 ng/ml), recombinant bovine interferon-α1 (rbIFNα; 1-1000 ng/ml), recombinant human interferon-α2b (rhIFNα; 100 ng/ml), or ovine interferon-τ (oIFNτ; 100 ng/ml). Proliferation was determined by monitoring uptake of [3H]thymidine into DNA. Generally, interferons did not inhibit proliferation of endometrial epithelial cells. Exceptions were for 1000 ng/ml rbIFNτ, which inhibited proliferation by 23%; 100 ng/ml rbIFNα, which inhibited proliferation by 28% in one of two experiments only; and 100 ng/ml oIFNτ, which inhibited proliferation by 17%. Proliferation of endometrial stromal cells was not inhibited by any concentration of any interferon in two separate experiments. Therefore, unlike other bovine cells tested previously (lymphocytes and oviductal cells), bovine endometrial cells were not consistently inhibited by IFNτ or IFNα. Such reduced responsiveness of endometrial cells to the antiproliferative effects of type 1 interferons could allow for growth of the endometrium during the period of pregnancy when the conceptus produces IFNτ.

UR - http://www.scopus.com/inward/record.url?scp=0028168729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028168729&partnerID=8YFLogxK

U2 - 10.1095/biolreprod51.4.700

DO - 10.1095/biolreprod51.4.700

M3 - Article

C2 - 7819452

AN - SCOPUS:0028168729

VL - 51

SP - 700

EP - 705

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 4

ER -