Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

Zhenyang Zheng, Yan Leng, Chen Zhou, Zhenyu Ma, Zhigang Zhong, Xing Ming Shi, Weixi Zhang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.

Original languageEnglish (US)
Pages (from-to)309-314
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume428
Issue number2
DOIs
StatePublished - Nov 16 2012

Fingerprint

Matrix Metalloproteinase 1
Stem cells
Mesenchymal Stromal Cells
Bone
Bone Marrow
MyoD Protein
Endopeptidases
Desmin
Muscle Development
Myoblasts
Inbred C57BL Mouse
Extracellular Matrix
Muscle
In Vitro Techniques
Zinc
Regeneration
Proteins
Muscles
Messenger RNA

Keywords

  • Bone marrow-derived mesenchymal stem cells
  • Matrix metalloproteinase-1
  • Myogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro. / Zheng, Zhenyang; Leng, Yan; Zhou, Chen; Ma, Zhenyu; Zhong, Zhigang; Shi, Xing Ming; Zhang, Weixi.

In: Biochemical and Biophysical Research Communications, Vol. 428, No. 2, 16.11.2012, p. 309-314.

Research output: Contribution to journalArticle

Zheng, Zhenyang ; Leng, Yan ; Zhou, Chen ; Ma, Zhenyu ; Zhong, Zhigang ; Shi, Xing Ming ; Zhang, Weixi. / Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro. In: Biochemical and Biophysical Research Communications. 2012 ; Vol. 428, No. 2. pp. 309-314.
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