Effects of matrix metalloproteinase-1 on the myogenic differentiation of bone marrow-derived mesenchymal stem cells in vitro

Zhenyang Zheng, Yan Leng, Chen Zhou, Zhenyu Ma, Zhigang Zhong, Xing Ming Shi, Weixi Zhang

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Matrix metalloproteinase-1 (MMP-1) is a member of the family of zinc-dependent endopeptidases that are capable of degrading extracellular matrix (ECM) and certain non-matrix proteins. It has been shown that MMP-1 can enhance muscle regeneration by improving the differentiation and migration of myoblasts. However, it is still not known whether MMP-1 can promote the myogenesis of bone marrow-derived mesenchymal stem cells (BMSCs). To address this question, we isolated BMSCs from C57BL/6J mice and investigated the effects of MMP-1 on their proliferation and myogenic differentiation. Our results showed that MMP-1 treatment, which had no cytotoxic effects on BMSCs, increased the mRNA and protein levels of MyoD and desmin in a dose-dependent manner, indicating that MMP-1 promoted myogenic differentiation of BMSCs in vitro. These results suggest that BMSCs may have a therapeutic potential for treating muscular disorders.

Original languageEnglish (US)
Pages (from-to)309-314
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume428
Issue number2
DOIs
StatePublished - Nov 16 2012

Keywords

  • Bone marrow-derived mesenchymal stem cells
  • Matrix metalloproteinase-1
  • Myogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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