Effects of solvents on the early stage stiffening rate of demineralized dentin matrix

Fernanda C.P. Garcia, Masayuki Otsuki, David H. Pashley, Franklin Chi Meng Tay, Ricardo M. Carvalho

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objectives. To monitor the stiffening rate of demineralized dentin matrix at the early stages after exposure to different neat solvents. Methods. Dentin beams approximately 0.8×0.7×8.0 mm were obtained from human third molars. After covering their ends with resin composite, the middle exposed length of 4.0 mm (gauge-length) was demineralized in 0.5 M EDTA (pH 7.0) for 7 days. The specimens were gripped by a testing machine, pre-loaded to 10 g and cyclically stressed in tension to 5% strain, for 30 repeated cycles (total 20 min) at 0.6 mm/min while immersed in water (control). Then, water was replaced by either 100% acetone, methanol, ethanol, propanol, HEMA or air and the specimens subjected to the same cyclic protocol. The maximum apparent modulus of elasticity (EMax) was calculated for every cycle, plotted as a function of time and subjected to regression analysis. Stiffening rate was calculated as changes in E (min). Regression analysis examined the relationship between E and time for each solvent. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test at α=0.05. Results. Regression analysis showed that E increased significantly with time in all water-free solvents (R 2=0.8-0.99). Stiffening rate was higher for acetone (0.9 MPa/min) and ethanol (0.8 MPa/min), intermediate for air (0.7 MPa/min), methanol (0.6 MPa/min) and propanol (0.5 MPa/min), lower for HEMA (0.2 MPa/min) and practically none for water (0.07 MPa/min) with p<0.05. Conclusions. The solvent-induced stiffening rate of demineralized dentin matrix is both time and solvent-dependent. The ability of solvents to promptly stiffen the demineralized dentin matrix may be important in maintaining the resin-infiltrated matrix expanded during the solvent evaporation stage of resin bonding.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalJournal of Dentistry
Volume33
Issue number5
DOIs
StatePublished - Jan 1 2005

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1-Propanol
Water
Regression Analysis
Acetone
Methanol
Ethanol
Air
Third Molar
Composite Resins
Elastic Modulus
Dentin
Demineralized Dentin Matrix
Edetic Acid
Analysis of Variance
Students
hydroxyethyl methacrylate

Keywords

  • Acetone
  • Air
  • Collagen
  • Ethanol
  • HEMA
  • Methanol
  • Modulus of elasticity
  • Propanol
  • Stiffening rate

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Effects of solvents on the early stage stiffening rate of demineralized dentin matrix. / Garcia, Fernanda C.P.; Otsuki, Masayuki; Pashley, David H.; Tay, Franklin Chi Meng; Carvalho, Ricardo M.

In: Journal of Dentistry, Vol. 33, No. 5, 01.01.2005, p. 371-377.

Research output: Contribution to journalArticle

Garcia, Fernanda C.P. ; Otsuki, Masayuki ; Pashley, David H. ; Tay, Franklin Chi Meng ; Carvalho, Ricardo M. / Effects of solvents on the early stage stiffening rate of demineralized dentin matrix. In: Journal of Dentistry. 2005 ; Vol. 33, No. 5. pp. 371-377.
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abstract = "Objectives. To monitor the stiffening rate of demineralized dentin matrix at the early stages after exposure to different neat solvents. Methods. Dentin beams approximately 0.8×0.7×8.0 mm were obtained from human third molars. After covering their ends with resin composite, the middle exposed length of 4.0 mm (gauge-length) was demineralized in 0.5 M EDTA (pH 7.0) for 7 days. The specimens were gripped by a testing machine, pre-loaded to 10 g and cyclically stressed in tension to 5{\%} strain, for 30 repeated cycles (total 20 min) at 0.6 mm/min while immersed in water (control). Then, water was replaced by either 100{\%} acetone, methanol, ethanol, propanol, HEMA or air and the specimens subjected to the same cyclic protocol. The maximum apparent modulus of elasticity (EMax) was calculated for every cycle, plotted as a function of time and subjected to regression analysis. Stiffening rate was calculated as changes in E (min). Regression analysis examined the relationship between E and time for each solvent. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test at α=0.05. Results. Regression analysis showed that E increased significantly with time in all water-free solvents (R 2=0.8-0.99). Stiffening rate was higher for acetone (0.9 MPa/min) and ethanol (0.8 MPa/min), intermediate for air (0.7 MPa/min), methanol (0.6 MPa/min) and propanol (0.5 MPa/min), lower for HEMA (0.2 MPa/min) and practically none for water (0.07 MPa/min) with p<0.05. Conclusions. The solvent-induced stiffening rate of demineralized dentin matrix is both time and solvent-dependent. The ability of solvents to promptly stiffen the demineralized dentin matrix may be important in maintaining the resin-infiltrated matrix expanded during the solvent evaporation stage of resin bonding.",
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AU - Otsuki, Masayuki

AU - Pashley, David H.

AU - Tay, Franklin Chi Meng

AU - Carvalho, Ricardo M.

PY - 2005/1/1

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N2 - Objectives. To monitor the stiffening rate of demineralized dentin matrix at the early stages after exposure to different neat solvents. Methods. Dentin beams approximately 0.8×0.7×8.0 mm were obtained from human third molars. After covering their ends with resin composite, the middle exposed length of 4.0 mm (gauge-length) was demineralized in 0.5 M EDTA (pH 7.0) for 7 days. The specimens were gripped by a testing machine, pre-loaded to 10 g and cyclically stressed in tension to 5% strain, for 30 repeated cycles (total 20 min) at 0.6 mm/min while immersed in water (control). Then, water was replaced by either 100% acetone, methanol, ethanol, propanol, HEMA or air and the specimens subjected to the same cyclic protocol. The maximum apparent modulus of elasticity (EMax) was calculated for every cycle, plotted as a function of time and subjected to regression analysis. Stiffening rate was calculated as changes in E (min). Regression analysis examined the relationship between E and time for each solvent. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test at α=0.05. Results. Regression analysis showed that E increased significantly with time in all water-free solvents (R 2=0.8-0.99). Stiffening rate was higher for acetone (0.9 MPa/min) and ethanol (0.8 MPa/min), intermediate for air (0.7 MPa/min), methanol (0.6 MPa/min) and propanol (0.5 MPa/min), lower for HEMA (0.2 MPa/min) and practically none for water (0.07 MPa/min) with p<0.05. Conclusions. The solvent-induced stiffening rate of demineralized dentin matrix is both time and solvent-dependent. The ability of solvents to promptly stiffen the demineralized dentin matrix may be important in maintaining the resin-infiltrated matrix expanded during the solvent evaporation stage of resin bonding.

AB - Objectives. To monitor the stiffening rate of demineralized dentin matrix at the early stages after exposure to different neat solvents. Methods. Dentin beams approximately 0.8×0.7×8.0 mm were obtained from human third molars. After covering their ends with resin composite, the middle exposed length of 4.0 mm (gauge-length) was demineralized in 0.5 M EDTA (pH 7.0) for 7 days. The specimens were gripped by a testing machine, pre-loaded to 10 g and cyclically stressed in tension to 5% strain, for 30 repeated cycles (total 20 min) at 0.6 mm/min while immersed in water (control). Then, water was replaced by either 100% acetone, methanol, ethanol, propanol, HEMA or air and the specimens subjected to the same cyclic protocol. The maximum apparent modulus of elasticity (EMax) was calculated for every cycle, plotted as a function of time and subjected to regression analysis. Stiffening rate was calculated as changes in E (min). Regression analysis examined the relationship between E and time for each solvent. Data were analyzed by one-way ANOVA and Student-Newman-Keuls test at α=0.05. Results. Regression analysis showed that E increased significantly with time in all water-free solvents (R 2=0.8-0.99). Stiffening rate was higher for acetone (0.9 MPa/min) and ethanol (0.8 MPa/min), intermediate for air (0.7 MPa/min), methanol (0.6 MPa/min) and propanol (0.5 MPa/min), lower for HEMA (0.2 MPa/min) and practically none for water (0.07 MPa/min) with p<0.05. Conclusions. The solvent-induced stiffening rate of demineralized dentin matrix is both time and solvent-dependent. The ability of solvents to promptly stiffen the demineralized dentin matrix may be important in maintaining the resin-infiltrated matrix expanded during the solvent evaporation stage of resin bonding.

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KW - Air

KW - Collagen

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KW - HEMA

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KW - Modulus of elasticity

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