Elevated focal adhesion kinase expression facilitates oral tumor cell invasion

Galen B. Schneider, Zoya Bronislavovna Kurago, Rebecca Zaharias, Lynn M. Gruman, Michael D. Schaller, Mary J.C. Hendrix

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

BACKGROUND. Understanding the molecular mechanisms of metastasis is critical with respect to oral tumorigenesis. The focal adhesion kinase (FAK) is an intracellular tyrosine kinase associated with the regulation of cell growth, migration, and survival. The purpose of the current study was to determine whether elevated FAK expression in oral malignancies was associated with increased invasiveness and oral carcinoma. METHODS. Immunohistochemical analysis was used to assess levels of FAK expression in archived oral carcinoma tissue samples. Invasion assays after transfections were used to assess the effect of increased FAK expression on invasive potential of oral tumor cells. RESULTS. The human oral carcinoma cell line SCC25 was significantly more invasive (P < 0.05) and expressed higher levels of FAK compared with the less invasive human oral carcinoma cell line SCC15. FAK expression was 3.0-fold higher in the SCC15 cell line and 5.0-fold higher in the SCC25 cell line compared with normal epithelial cells. In the highly invasive SCC25 cell line, FAK expression was 1.5-fold higher compared with the less invasive SCC15 cell line. FAK immunostaining in oral tumorswas significantly more intense compared with the immunostaining in surrounding normal epithelium or chronic mucositis. Overexpression of FAK in low-invading SCC15 cells resulted in a 4.5-fold increase in the rate of invasion compared with untransfected or neotransfected control SCC15 cell lines and a nearly 1.5-fold greater rate compared with the highly invasive untransfected SCC25 cell line. CONCLUSIONS. The current results suggest that enhanced expression of FAK in oral carcinoma cells may lead to a selective growth advantage and increased invasive potential of the primary oral tumor.

Original languageEnglish (US)
Pages (from-to)2508-2515
Number of pages8
JournalCancer
Volume95
Issue number12
DOIs
StatePublished - Dec 15 2002

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Cell Line
Neoplasms
Carcinoma
Mucositis
Growth
Protein-Tyrosine Kinases
Cell Movement
Transfection
Cell Survival
Carcinogenesis
Epithelium
Epithelial Cells
Neoplasm Metastasis

Keywords

  • Carcinoma
  • Focal adhesion kinase
  • Invasion
  • Metastasis
  • Oral

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Schneider, G. B., Kurago, Z. B., Zaharias, R., Gruman, L. M., Schaller, M. D., & Hendrix, M. J. C. (2002). Elevated focal adhesion kinase expression facilitates oral tumor cell invasion. Cancer, 95(12), 2508-2515. https://doi.org/10.1002/cncr.10992

Elevated focal adhesion kinase expression facilitates oral tumor cell invasion. / Schneider, Galen B.; Kurago, Zoya Bronislavovna; Zaharias, Rebecca; Gruman, Lynn M.; Schaller, Michael D.; Hendrix, Mary J.C.

In: Cancer, Vol. 95, No. 12, 15.12.2002, p. 2508-2515.

Research output: Contribution to journalArticle

Schneider, GB, Kurago, ZB, Zaharias, R, Gruman, LM, Schaller, MD & Hendrix, MJC 2002, 'Elevated focal adhesion kinase expression facilitates oral tumor cell invasion', Cancer, vol. 95, no. 12, pp. 2508-2515. https://doi.org/10.1002/cncr.10992
Schneider GB, Kurago ZB, Zaharias R, Gruman LM, Schaller MD, Hendrix MJC. Elevated focal adhesion kinase expression facilitates oral tumor cell invasion. Cancer. 2002 Dec 15;95(12):2508-2515. https://doi.org/10.1002/cncr.10992
Schneider, Galen B. ; Kurago, Zoya Bronislavovna ; Zaharias, Rebecca ; Gruman, Lynn M. ; Schaller, Michael D. ; Hendrix, Mary J.C. / Elevated focal adhesion kinase expression facilitates oral tumor cell invasion. In: Cancer. 2002 ; Vol. 95, No. 12. pp. 2508-2515.
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N2 - BACKGROUND. Understanding the molecular mechanisms of metastasis is critical with respect to oral tumorigenesis. The focal adhesion kinase (FAK) is an intracellular tyrosine kinase associated with the regulation of cell growth, migration, and survival. The purpose of the current study was to determine whether elevated FAK expression in oral malignancies was associated with increased invasiveness and oral carcinoma. METHODS. Immunohistochemical analysis was used to assess levels of FAK expression in archived oral carcinoma tissue samples. Invasion assays after transfections were used to assess the effect of increased FAK expression on invasive potential of oral tumor cells. RESULTS. The human oral carcinoma cell line SCC25 was significantly more invasive (P < 0.05) and expressed higher levels of FAK compared with the less invasive human oral carcinoma cell line SCC15. FAK expression was 3.0-fold higher in the SCC15 cell line and 5.0-fold higher in the SCC25 cell line compared with normal epithelial cells. In the highly invasive SCC25 cell line, FAK expression was 1.5-fold higher compared with the less invasive SCC15 cell line. FAK immunostaining in oral tumorswas significantly more intense compared with the immunostaining in surrounding normal epithelium or chronic mucositis. Overexpression of FAK in low-invading SCC15 cells resulted in a 4.5-fold increase in the rate of invasion compared with untransfected or neotransfected control SCC15 cell lines and a nearly 1.5-fold greater rate compared with the highly invasive untransfected SCC25 cell line. CONCLUSIONS. The current results suggest that enhanced expression of FAK in oral carcinoma cells may lead to a selective growth advantage and increased invasive potential of the primary oral tumor.

AB - BACKGROUND. Understanding the molecular mechanisms of metastasis is critical with respect to oral tumorigenesis. The focal adhesion kinase (FAK) is an intracellular tyrosine kinase associated with the regulation of cell growth, migration, and survival. The purpose of the current study was to determine whether elevated FAK expression in oral malignancies was associated with increased invasiveness and oral carcinoma. METHODS. Immunohistochemical analysis was used to assess levels of FAK expression in archived oral carcinoma tissue samples. Invasion assays after transfections were used to assess the effect of increased FAK expression on invasive potential of oral tumor cells. RESULTS. The human oral carcinoma cell line SCC25 was significantly more invasive (P < 0.05) and expressed higher levels of FAK compared with the less invasive human oral carcinoma cell line SCC15. FAK expression was 3.0-fold higher in the SCC15 cell line and 5.0-fold higher in the SCC25 cell line compared with normal epithelial cells. In the highly invasive SCC25 cell line, FAK expression was 1.5-fold higher compared with the less invasive SCC15 cell line. FAK immunostaining in oral tumorswas significantly more intense compared with the immunostaining in surrounding normal epithelium or chronic mucositis. Overexpression of FAK in low-invading SCC15 cells resulted in a 4.5-fold increase in the rate of invasion compared with untransfected or neotransfected control SCC15 cell lines and a nearly 1.5-fold greater rate compared with the highly invasive untransfected SCC25 cell line. CONCLUSIONS. The current results suggest that enhanced expression of FAK in oral carcinoma cells may lead to a selective growth advantage and increased invasive potential of the primary oral tumor.

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