Elimination of epithelial-like and mesenchymal-like breast cancer stem cells to inhibit metastasis following nanoparticle-mediated photothermal therapy

Hayley J. Paholak, Nicholas O. Stevers, Hongwei Chen, Joseph P. Burnett, Miao He, Hasan Korkaya, Sean P. McDermott, Yadwinder Deol, Shawn G. Clouthier, Tahra Luther, Qiao Li, Max S. Wicha, Duxin Sun

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Increasing evidence suggesting breast cancer stem cells (BCSCs) drive metastasis and evade traditional therapies underscores a critical need to exploit the untapped potential of nanotechnology to develop innovative therapies that will significantly improve patient survival. Photothermal therapy (PTT) to induce localized hyperthermia is one of few nanoparticle-based treatments to enter clinical trials in human cancer patients, and has recently gained attention for its ability to induce a systemic immune response targeting distal cancer cells in mouse models. Here, we first conduct classic cancer stem cell (CSC) assays, both in vitro and in immune-compromised mice, to demonstrate that PTT mediated by highly crystallized iron oxide nanoparticles effectively eliminates BCSCs in translational models of triple negative breast cancer. PTT in vitro preferentially targets epithelial-like ALDH + BCSCs, followed by mesenchymal-like CD44+/CD24- BCSCs, compared to bulk cancer cells. PTT inhibits BCSC self-renewal through reduction of mammosphere formation in primary and secondary generations. Secondary implantation in NOD/SCID mice reveals the ability of PTT to impede BCSC-driven tumor formation. Next, we explore the translational potential of PTT using metastatic and immune-competent mouse models. PTT to inhibit BCSCs significantly reduces metastasis to the lung and lymph nodes. In immune-competent BALB/c mice, PTT effectively eliminates ALDH + BCSCs. These results suggest the feasibility of incorporating PTT into standard clinical treatments such as surgery to enhance BCSC destruction and inhibit metastasis, and the potential of such combination therapy to improve long-term survival in patients with metastatic breast cancer.

Original languageEnglish (US)
Pages (from-to)145-157
Number of pages13
JournalBiomaterials
Volume104
DOIs
StatePublished - Oct 1 2016

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Neoplastic Stem Cells
Stem cells
Nanoparticles
Breast Neoplasms
Neoplasm Metastasis
Therapeutics
Cells
Neoplasms
Colony-Forming Units Assay
Triple Negative Breast Neoplasms
Iron oxides
Nanotechnology
Inbred NOD Mouse
Investigational Therapies
Surgery
SCID Mice
Survival
Tumors
Assays
Fever

Keywords

  • Breast cancer stem cells
  • Iron oxide nanoparticles
  • Metastasis inhibition
  • Photothermal therapy
  • Triple negative breast cancer

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Elimination of epithelial-like and mesenchymal-like breast cancer stem cells to inhibit metastasis following nanoparticle-mediated photothermal therapy. / Paholak, Hayley J.; Stevers, Nicholas O.; Chen, Hongwei; Burnett, Joseph P.; He, Miao; Korkaya, Hasan; McDermott, Sean P.; Deol, Yadwinder; Clouthier, Shawn G.; Luther, Tahra; Li, Qiao; Wicha, Max S.; Sun, Duxin.

In: Biomaterials, Vol. 104, 01.10.2016, p. 145-157.

Research output: Contribution to journalArticle

Paholak, HJ, Stevers, NO, Chen, H, Burnett, JP, He, M, Korkaya, H, McDermott, SP, Deol, Y, Clouthier, SG, Luther, T, Li, Q, Wicha, MS & Sun, D 2016, 'Elimination of epithelial-like and mesenchymal-like breast cancer stem cells to inhibit metastasis following nanoparticle-mediated photothermal therapy', Biomaterials, vol. 104, pp. 145-157. https://doi.org/10.1016/j.biomaterials.2016.06.045
Paholak, Hayley J. ; Stevers, Nicholas O. ; Chen, Hongwei ; Burnett, Joseph P. ; He, Miao ; Korkaya, Hasan ; McDermott, Sean P. ; Deol, Yadwinder ; Clouthier, Shawn G. ; Luther, Tahra ; Li, Qiao ; Wicha, Max S. ; Sun, Duxin. / Elimination of epithelial-like and mesenchymal-like breast cancer stem cells to inhibit metastasis following nanoparticle-mediated photothermal therapy. In: Biomaterials. 2016 ; Vol. 104. pp. 145-157.
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