Emission and polarization spectrometer for biophysical spectroscopy

John Clark Sutherland, George D. Cimino, John T. Lowe

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We have constructed a spectrometer which, using well established techniques, measures both fluorescence and circular dichroism (natural and magnetic) as well as several other parameters of interest in biophysical spectroscopy. This combination is feasible since the various measurements, aside from sharing many optical components, can all use a lock-in amplifier to process electronic signals. Although fluorescence and circular dichroism provide greatly different information about the material under investigation, from the standpoint of instrumentation they are very similar. Combining several functions in a single spectrometer has the advantages of economy and flexibility.

Original languageEnglish (US)
Pages (from-to)358-360
Number of pages3
JournalReview of Scientific Instruments
Volume47
Issue number3
DOIs
StatePublished - Dec 1 1976
Externally publishedYes

ASJC Scopus subject areas

  • Instrumentation

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