Emission and polarization spectrometer for biophysical spectroscopy

John C. Sutherland, George D. Cimino, John T. Lowe

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We have constructed a spectrometer which, using well established techniques, measures both fluorescence and circular dichroism (natural and magnetic) as well as several other parameters of interest in biophysical spectroscopy. This combination is feasible since the various measurements, aside from sharing many optical components, can all use a lock-in amplifier to process electronic signals. Although fluorescence and circular dichroism provide greatly different information about the material under investigation, from the standpoint of instrumentation they are very similar. Combining several functions in a single spectrometer has the advantages of economy and flexibility.

Original languageEnglish (US)
Pages (from-to)358-360
Number of pages3
JournalReview of Scientific Instruments
Volume47
Issue number3
DOIs
StatePublished - Dec 1 1976

Fingerprint

Dichroism
dichroism
Spectrometers
Fluorescence
Spectroscopy
spectrometers
Polarization
fluorescence
economy
polarization
spectroscopy
flexibility
amplifiers
electronics

ASJC Scopus subject areas

  • Instrumentation

Cite this

Emission and polarization spectrometer for biophysical spectroscopy. / Sutherland, John C.; Cimino, George D.; Lowe, John T.

In: Review of Scientific Instruments, Vol. 47, No. 3, 01.12.1976, p. 358-360.

Research output: Contribution to journalArticle

Sutherland, John C. ; Cimino, George D. ; Lowe, John T. / Emission and polarization spectrometer for biophysical spectroscopy. In: Review of Scientific Instruments. 1976 ; Vol. 47, No. 3. pp. 358-360.
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