Endocytosis and degradation of interstitial retinol-binding protein

Differential capabilities of cells that border the interphotoreceptor matrix

Joe G. Hollyfield, Hugh H. Varner, Mary E. Rayborn, Gregory I Liou, C. David Bridges

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Between the pigment epithelium and the outer limiting membrane of the retina is an extracellular compartment filled with the interphotoreceptor matrix (IPM). A prominent component of the IPM is a glycoprotein known as interstitial retinol-binding protein (IRBP). Using in vitro techniques, we compared the ability of the cells that border this compartment to internalize colloidal gold (CG) coated with either IRBP or ovalbumin, a glycoprotein not found in the IPM. Neither IRBP-CG nor ovalbumin-CG was internalized by the Muller’s cells. Both rod and cone photoreceptors take up IRBP-CG, which is observed in small vesicles and multivesicular bodies. Neither photoreceptor type takes up ovalbumin-CG. Acid phosphatase cytochemistry indicates that acid phosphatase reaction product in the multivesicular bodies co-localizes with IRBP-CG, which suggests that this molecule is degraded by rod and cone photoreceptors and is not recycled. The pigment epithelium internalizes IRBP-CG and ovalbumin-CG, both of which remain in small cytoplasmic vesicles near the apical plasma membrane. There is no indication that vesicles that contain either IRBP-CG or ovalbumin-CG fuse with the lysosomal system in the pigment epithelial cells during the incubation.

Original languageEnglish (US)
Pages (from-to)1676-1681
Number of pages6
JournalJournal of Cell Biology
Volume100
Issue number5
DOIs
StatePublished - May 1 1985

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Gold Colloid
Endocytosis
Ovalbumin
Multivesicular Bodies
Retinal Cone Photoreceptor Cells
Retinal Rod Photoreceptor Cells
Vertebrate Photoreceptor Cells
Acid Phosphatase
Glycoproteins
Epithelium
interstitial retinol-binding protein
Cytoplasmic Vesicles
Histocytochemistry
Retina
Epithelial Cells
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

Endocytosis and degradation of interstitial retinol-binding protein : Differential capabilities of cells that border the interphotoreceptor matrix. / Hollyfield, Joe G.; Varner, Hugh H.; Rayborn, Mary E.; Liou, Gregory I; David Bridges, C.

In: Journal of Cell Biology, Vol. 100, No. 5, 01.05.1985, p. 1676-1681.

Research output: Contribution to journalArticle

Hollyfield, Joe G. ; Varner, Hugh H. ; Rayborn, Mary E. ; Liou, Gregory I ; David Bridges, C. / Endocytosis and degradation of interstitial retinol-binding protein : Differential capabilities of cells that border the interphotoreceptor matrix. In: Journal of Cell Biology. 1985 ; Vol. 100, No. 5. pp. 1676-1681.
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